1968
DOI: 10.1139/m68-111
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Mitomycin C induction of a temperate phage in Pseudomonas aeruginosa

Abstract: After addition of mitomycin C to Pseudomonas aeruginosa strain MAC-264 cells, DNA synthesis stops but resumes again after 45 min. This resumption in DNA synthesis is due to the induction of a temperate phage. Early after induction, both RNA and protein syntheses continue but later on little or no net synthesis of these macromolecules occurs. Cell lysis occurs at 90 to 105 min after addition of mitomycin C. Analysis of the lysates indicates that a DNA phage with an icosahedral head about 55 mμ in diameter with … Show more

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Cited by 15 publications
(12 citation statements)
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“…As expected, these findings demonstrate that both CIP and MMC can induce an SOS-like response in B. thailandensis. However, the number of SOS-regulated genes that are induced or repressed by CIP and MMC in B. thailandensis is not as high as what has been reported for E. coli, Pseudomonas spp., and S. aureus (7,8,23,(25)(26)(27)(28)(29). For example, in S. aureus, CIP induces the expression of numerous protein-encoding genes involved in DNA metabolism, including lexA and recA, in addition to uvrA, uvrB, parE, parC, recF, gyrA, and gyrB (29).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…As expected, these findings demonstrate that both CIP and MMC can induce an SOS-like response in B. thailandensis. However, the number of SOS-regulated genes that are induced or repressed by CIP and MMC in B. thailandensis is not as high as what has been reported for E. coli, Pseudomonas spp., and S. aureus (7,8,23,(25)(26)(27)(28)(29). For example, in S. aureus, CIP induces the expression of numerous protein-encoding genes involved in DNA metabolism, including lexA and recA, in addition to uvrA, uvrB, parE, parC, recF, gyrA, and gyrB (29).…”
Section: Resultsmentioning
confidence: 99%
“…It has been well documented in the literature that DNA damage and induction of the SOS response correlates with the lytic bacteriophage cycle (26,(40)(41)(42)(43). B. thailandensis E264 encodes three prophage-like islands designated PI E264-1, PI E264-2, and PI E264-3 (44).…”
Section: Resultsmentioning
confidence: 99%
“…Details about the generation of the strains, the cultivation and the conditions during biosensing are given in the Supporting Information. For the development and testing of the cell‐based biosensor, Mitomycin C (Otsuji et al ., 1959; Yamamoto and Chow, 1968) was selected as an example for an organic inducer, whereas Cis‐platinum ((SP‐4‐2)‐diamminedichloroplatinum(II)) and hexavalent chromium (chromate) served as examples for inorganic inducers. All test substances are well‐known prophage‐activating chemicals.…”
Section: Methodsmentioning
confidence: 99%
“…Korsten et al (1979) have remarked on the amazing similarity of structure and intracellular replication strategy between virulent members of this viral group isolated against a variety of phylogenetically unrelated bacteria. There has been only one report in the scientific literature on the isolation and characterization of temperate members of the Podoviridae infectious for Pseudomonas aeruginosa (Yamamoto and Chow 1968;H.-W. Ackermann, personal communication). Enrich-'Author to whom correspondence should be addressed.…”
Section: Introductionmentioning
confidence: 99%