Isolation and characterization of a <i>Pseudomonas aeruginosa</i> bacteriophage with a very limited host range

Bigby, Dianne; Kropinski, Andrew M.

doi:10.1139/m89-101

Cited by 9 publications

(6 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Head and tail dimensions of these phages closely resembled with some of the earlier reported Vibriophages such as N1, N2, N3, N4, N5 and S5 belonging to Podoviridae family [44,25]. The findings of this study also support the observations made by earlier workers where phages such as ФPLS743, LK16, LKA1 and ФKMV against Pseudomonas aeruginosa belonging to Podoviridae family were isolated [45][46][47]. To the best of our knowledge, this is the first report on isolation and characterization of bacteriophages specific to Pseudomonas aeruginosa PAO, belonging to family Podoviridae from India.…”

Section: Discussionsupporting

confidence: 90%

Characterization of Pseudomonas aeruginosa PAO Specific Bacteriophages Isolated from Sewage Samples

Kumari¹,

Harjai²,

Chhibber³

2009

Am. J. Biomed. Sci.

View full text Add to dashboard Cite

The purpose of this study was to isolate and characterize Pseudomonas aeruginosa phages with regard to their morphology, growth characteristics, genetic material and structural protein composition. Five phages specific to P. aeruginosa PAO were isolated from sewage samples. Adsorption rates and one step growth curves of these phages were determined and on this basis, phages were found to be distinct entities. DNA of all the phages subjected to restriction enzyme digestion, confirmed the presence of double stranded (ds) DNA as well as heterogeneity among these phages. RAPD PCR analysis of the phage DNA also revealed difference among all the 5 phages as distinct banding patterns were seen in each case. Transmission electron microscopy (TEM) however, showed morphological similarity among the phages as all were assigned to family Podoviridae, order Caudovirales on the basis of their head and tail morphologies, with some size variations. Earlier reports revealed that Pseudomonas phages either belonged to family Siphoviridae, Myoviridae or Podoviridae. However, in the present study, we report isolation of P. aeruginosa phages belonging to family Podoviridae with icosahederal heads and short stumpy non-contractile tails first time from India.

show abstract

Section: Discussionsupporting

confidence: 90%

Characterization of Pseudomonas aeruginosa PAO Specific Bacteriophages Isolated from Sewage Samples

Kumari¹,

Harjai²,

Chhibber³

2009

Am. J. Biomed. Sci.

View full text Add to dashboard Cite

show abstract

“…Although phages infecting strains of two or more species of a particular genus have been described, the results to date indicate that most of them are host species-specific and also demonstrate strain specificity [4,8,10]. All of the isolated phages in this experiment infected only Xoo strains.…”

Section: Discussionmentioning

confidence: 61%

Diversity of Bacteriophages Infecting Xanthomonas oryzae pv. oryzae in Paddy Fields and Its Potential to Control Bacterial Leaf Blight of Rice

Chae¹,

Hung²,

Yu³

et al. 2014

Journal of Microbiology and Biotechnology

View full text Add to dashboard Cite

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a very serious disease in rice-growing regions of the world. In spite of their economic importance, there are no effective ways of protecting rice plants from this disease. Bacteriophages infecting Xoo affect the population dynamics of the pathogen and consequently the occurrence of the disease. In this study, we investigated the diversity, host range, and infectivity of Xoo phages, and their use as a bicontrol agent on BLB was tested. Among the 34 phages that were isolated from floodwater in paddy fields, 29 belonged to the Myoviridae family, which suggests that the dominant phage in the ecosystem was Myoviridae. The isolated phages were classified into two groups based on plaque size produced on the lawn of Xoo. In general, there was a negative relationship between plaque size and host range, and interestingly the phages having a narrow host range had low efficiency of infectivity. The deduced protein sequence analysis of htf genes indicated that the gene was not a determinant of host specificity. Although the difference in host range and infectivity depending on morphotype needs to be addressed, the results revealed deeper understanding of the interaction between the phages and Xoo strains in floodwater and damp soil environments. The phage mixtures reduced the occurrence of BLB when they were treated with skim milk. The results indicate that the Xoo phages could be used as an alternative control method to increase the control efficacy and reduce the use of agrochemicals.

show abstract

“…Among the strains tested it lysed only P. fluorescens R12t, which is very similar to strain R2f. This specificity is not surprising because many bacteriophages have a limited host range, which in some cases is used for strain typing (1,3). Therefore, this phage was considered to be sufficiently specific to selectively kill off the introduced donor cells, leaving undisturbed the majority of the indigenous soil bacteria.…”

Section: Discussionmentioning

confidence: 99%

Detection of Plasmid Transfer from Pseudomonas fluorescens to Indigenous Bacteria in Soil by Using Bacteriophage φR2f for Donor Counterselection

Smit

Elsas

Veen

et al. 1991

Appl Environ Microbiol

105

View full text Add to dashboard Cite

The transfer of a genetically marked derivative of plasmid RP4, RP4p, from Pseudomonas fluorescens to members of the indigenous microflora of the wheat rhizosphere was studied by using a bacteriophage that specifically lyses the donor strain and a specific eukaryotic marker on the plasmid. Transfer of RP4p to the wheat rhizosphere microflora was observed, and the number of transconjugants detected was approximately 10 3 transconjugants per g of soil when 10 7 donor cells per g of soil were added; transfer in the corresponding bulk soil was slightly above the limit of detection. All of the indigenous transconjugants which we analyzed contained a 60-kb plasmid and were able to transfer this plasmid to a Nx r Rp r P. fluorescens recipient strain. The indigenous transconjugants were identified as belonging to Pseudomonas spp., Enterobacter spp., Comamonas spp., and Alcaligenes spp.

show abstract

Isolation and characterization of a Pseudomonas aeruginosa bacteriophage with a very limited host range

Cited by 9 publications

References 36 publications

Characterization of Pseudomonas aeruginosa PAO Specific Bacteriophages Isolated from Sewage Samples

Characterization of Pseudomonas aeruginosa PAO Specific Bacteriophages Isolated from Sewage Samples

Diversity of Bacteriophages Infecting Xanthomonas oryzae pv. oryzae in Paddy Fields and Its Potential to Control Bacterial Leaf Blight of Rice

Detection of Plasmid Transfer from Pseudomonas fluorescens to Indigenous Bacteria in Soil by Using Bacteriophage φR2f for Donor Counterselection

Contact Info

Product

Resources

About