Mitogen activated protein kinases (MAPKs) as regulators of spermatogenesis and spermatozoa functions

Almog, Tal; Naor, Zvi

doi:10.1016/j.mce.2007.11.011

Cited by 82 publications

(61 citation statements)

References 55 publications

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“…Mitogen-activated protein kinase (MAPK) is required for the activation of spermatozoa [38,39], which should be repressed when spermatozoa are still in the sperm reservoir [8,9]. The increase in TRAK1 RNA levels (Table 1 and Fig.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Transcript Expression Analysis in the Uterovaginal Junction in Association with Fertile Period in Tsaiya Ducks

Huang

Cheng

Yang

et al. 2011

Journal of Reproduction and Development

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Abstract. We performed the first genome-wide expression analysis to compare the differences in gene expression in the female sperm reservoir of the duck reproductive tract between two groups with long and short fertile periods to identify factors that may be associated with the fertile period using an oligonucleotide microarray. RNA was extracted from the uterovaginal junction (UV junction) of the two groups. Affymetrix chips containing comprehensive coverage of 32773 transcripts were hybridized with biotin-labeled cRNA, and three biological repeats were performed. We identified 27 transcripts as being differentially regulated. Interestingly, by mapping the differentially expressed transcripts to annotated pathways, we found that Neuropeptide Y (NPY), the RNA expression of which was increased by 2.96-fold in the short-fertile-period group as compared with the long-fertile-period group in our experiment, has been shown to reduce blood flow and substance supply to local tissues. Enah/Vasp-like (EVL), the RNA expression of which was significantly increased by 1.77-fold in the short-fertile-period group as compared with the long-period group, has been demonstrated to be important in activated T-cells. In contrast, trafficking kinesin-binding protein 1 (TRAK1), the expression of which was increased by 2.33-fold in the long-period group as compared with its counterparts, has been suggested to inhibit precocious activation of sperm and prolong sperm life in the female sperm reservoir. The results of real-time PCR confirmed the data obtained by microarray analysis. Our study demonstrated that combining global gene expression investigation with annotated pathway resources contributes to the understanding of sperm life when sustained in the UV junction.Key words: Differentially expressed transcripts, Duck, Fertile period, Uterovaginal junction (J. Reprod. Dev. 57: [731][732][733][734][735][736] 2011) A fter the entry of sperm into a female genital tract before ovulation, sperm accumulate to a certain amount and are stored in the female sperm reservoir [1], which is a widespread phenomenon among numerous avian, reptilian and mammalian species [2][3][4]. The sperm reservoir then releases sperm gradually for successful fertilization after a single insemination, the period of which varies from hours to weeks in different species [2,[4][5][6]. This period is defined as the fertile period. A long fertile period means prolonged sperm survival and leads to a high fertilization rate [7].The process of sperm migration in the female genital tract is more than simply transportation from the position of insemination to the position of fertilization. Rather, it is a complex continuum: entry into the lower genital tract, defense against the tract environment and immunological response, retention in the storage reservoir, activation, taxiing of activated sperm towards oocytes [8], and phagocytosis of nonfertilizing sperm. During sperm storage in the reservoir of the female reproductive tract, there is a close interaction between sperm...

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Transcript Expression Analysis in the Uterovaginal Junction in Association with Fertile Period in Tsaiya Ducks

Huang

Cheng

Yang

et al. 2011

Journal of Reproduction and Development

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“…In the present study, we extended these observations by exploring the expression of elements of the MAPK signaling cascade, which is known to play critical roles in Sertoli and germ cell functions (Almog and Naor, 2008;Wong and Cheng, 2005) and by examining the consequences of genistein and BPA fetal exposure on testicular cell populations at different ages.…”

Section: Introductionmentioning

confidence: 95%

Changes in MAPK pathway in neonatal and adult testis following fetal estrogen exposure and effects on rat testicular cells

Thuillier

Manku

Wang

et al. 2009

Microscopy Res & Technique

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Concerns have been raised about the possible role of the phytoestrogen genistein and the xenoestrogen Bisphenol A (BPA) as endocrine disruptors. In the present study, we examined the effects of fetal exposure to genistein and BPA on the Mitogen-activated protein kinase (MAPK) pathway and on testicular cell populations in neonatal and adult rat testes. At postnatal day (PND) 3, genistein (0.1-10 mg/kg/day) and BPA (1-200 mg/kg/day) induced Raf1 and Erk1/2 mRNA and protein increases in testes, mainly in Sertoli cells. No changes were seen for Mek1. At PND60, Erk1/2 protein expression remained robust in Sertoli cells and in some spermatogonia. Raf1 was predominant in Leydig cells while Mek1 was expressed strongly in spermatogonia, and they were both expressed in pachytene spermatocytes. No consistent change was seen in these proteins at PND60. Transient effects were observed on germ cell populations, while the only remaining effect on adult testicular cells was an increase in Leydig cell number. Rats exposed in utero to the two compounds did not present significant changes in circulating testosterone levels, suggesting normally functioning adult Leydig cells. Furthermore, Sertoli cell numbers were not affected by exposure to genistein and BPA. Finally, around 10% of genistein and BPA exposed rats were sterile, whereas all control rats were fertile. These data suggest that fetal exposure to genistein and BPA exerts transient effects in rat testes and that the changes observed at PND3 did not correlate with relevant changes in germ cell populations, Leydig cell function, or fertility in the adult.

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“…GILZ Regulates Ras Pathway in Undifferentiated Spermatogonia-It is known that the Ras signaling pathway is a key regulator of spermatogonia proliferation (41)(42)(43). Moreover, it has been shown previously that GILZ binds Ras and inhibits Ras-dependent cell proliferation (26).…”

Section: Increased Proliferation and Differentiation In Gilz Komentioning

confidence: 99%

Long Glucocorticoid-induced Leucine Zipper (L-GILZ) Protein Interacts with Ras Protein Pathway and Contributes to Spermatogenesis Control

Bruscoli

Velardi

Sante

et al. 2012

Journal of Biological Chemistry

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Background: Understanding how spermatogenesis occurs in mammals is not yet fully understood. Results: L-GILZ deficiency in germ cells leads to complete loss of germ cell lineage resulting in male sterility. Conclusion: Our study identifies L-GILZ as an important factor for spermatogenesis. Significance: Identification of genes critical for maintenance of spermatogenesis is pivotal for diagnosis and treatment of male infertility.

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Mitogen activated protein kinases (MAPKs) as regulators of spermatogenesis and spermatozoa functions

Cited by 82 publications

References 55 publications

Genome-Wide Transcript Expression Analysis in the Uterovaginal Junction in Association with Fertile Period in Tsaiya Ducks

Genome-Wide Transcript Expression Analysis in the Uterovaginal Junction in Association with Fertile Period in Tsaiya Ducks

Changes in MAPK pathway in neonatal and adult testis following fetal estrogen exposure and effects on rat testicular cells

Long Glucocorticoid-induced Leucine Zipper (L-GILZ) Protein Interacts with Ras Protein Pathway and Contributes to Spermatogenesis Control

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