Mitochondrial structural and antioxidant system responses to aging in oat (Avena sativa L.) seeds with different moisture contents

Xia, Fangshan; Wang, Xianguo; Li, Manli; Mao, Peisheng

doi:10.1016/j.plaphy.2015.06.002

Cited by 45 publications

(41 citation statements)

References 35 publications

(36 reference statements)

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“…2d-g). This was consistent with our previous findings, which showed that the integrity of the mitochondrial membrane was related to the levels of mitochondrial antioxidants and MDA contents in aged oat seeds [7]. ATP production in mitochondria is the main source of energy during seed germination [25], but oxidative modification can reduce the activities of COX and MDH during seed aging, leading to a decrease in the ATP production [23,24].…”

Section: Discussionsupporting

confidence: 92%

“…However, mitochondrial ultrastructural damage in response to artificial aging negatively impacts mitochondrial function [25]. Previous research also suggests that a decrease in the activities of antioxidant enzymes participating in the ASC-GSH cycle, accompanied by mitochondrial ultrastructural damage, is the major cause of aging in oat seeds [7]. Our current results showed that the negative influence of aging in oat seeds could be effectively repaired by post-priming with ASC, GSH, or ASC + GSH (Fig.…”

Section: Discussionsupporting

confidence: 60%

“…Once · O 2 − is generated by the electron transport chain during seed aging, it is promptly dismutated into hydrogen peroxide (H 2 O 2 ) by superoxide dismutase (SOD) in the matrix [5,6]. H 2 O 2 is then eliminated via the various antioxidant pathways that are crucial for the production and scavenging of ROS [7]. In particular, the scavenging of ROS largely depends on the availability of molecular antioxidants such as ascorbic acid (ASC) and glutathione (GSH) in dry seeds [8].…”

Section: Introductionmentioning

confidence: 99%

“…Similar to cellular antioxidant systems, the ASC-GSH cycle is also one of the major antioxidant protection systems in mitochondria under oxidative stresses [10,26]. A distinct reduction has been observed in the content of both ASC and GSH and the activities of ASC-GSH cycle enzymes during seed aging [7,25]. Nevertheless, there is still only a limited understanding of how to enhance mitochondrial function by improving the antioxidant capacity of the ASC-GSH cycle.…”

Section: Introductionmentioning

confidence: 99%

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Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds

et al. 2020

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Background: Loss of vigor caused by seed aging adversely affects agricultural production under natural conditions. However, priming is an economical and effective method for improving the vigor of aged seeds. The objective of this study was to test the effectiveness of exogenous ascorbic acid (ASC) and glutathione (GSH) priming in the repairing of aged oat (Avena sativa) seeds, and to test the hypothesis that structural and functional systems in mitochondria were involved in this process. Results: Oat seeds were artificially aged for 20 days at 45°C, and were primed with solutions (1 mmol L − 1 ) of ASC, GSH, or ASC + GSH at 20°C for 0.5 h before or after their aging. Seed germination, antioxidant enzymes in the ASC-GSH cycle, cytochrome c oxidase (COX) and mitochondrial malate dehydrogenase (MDH) activities, and the mitochondrial ultrastructures of the embryonic root cells were markedly improved in aged oat seeds through post-priming with ASC, GSH, or ASC + GSH, while their malondialdehyde and H 2 O 2 contents decreased significantly (P < 0.05). Conclusion: Our results suggested that priming with ASC, GSH, or ASC + GSH after aging could effectively alleviate aging damage in oat seeds, and that the role of ASC was more effective than GSH, but positive effects of post-priming with ASC and GSH were not superior to post-priming with ASC in repairing aging damage of aged oat seeds. However, pre-priming with ASC, GSH, or ASC + GSH was not effective in oat seeds, suggesting that pre-priming with ASC, GSH, or ASC + GSH could not inhibit the occurrence of aging damage in oat seeds.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds

et al. 2020

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“…Artificial ageing, that is storing seeds at high temperature and high relative humidity, can mimic natural ageing to study seed longevity and vigour (Xu et al, ). Reactive oxygen species (ROS) and lipid peroxidation are thought to be the major contributors to seed ageing, which include loss of membrane integrity, reduction in energy metabolism, decrease in antioxidant system activity, impairment of RNA and protein synthesis and DNA degradation (Xia, Wang, Li, & Mao, ; Zhang et al, ).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of different methods revealing the involvement of proteins during ageing of rice seeds

Jiang

Lian

et al. 2019

Plant Breeding

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Seed longevity is a very important characteristic controlling seed quality. However, the mechanism underlying this characteristic is poorly understood. In this study, 'FH7185', a storable rice variety, the germination rate of which was 66.63%, much higher than the control after artificial ageing under relative humidity 88% and 42°C for 21 days. Different methods were applied to reveal the involvement of proteins during ageing of rice seeds. In total, 35 differential proteins were identified from 2D‐PAGE, and 3,719 proteins from iTRAQ analysis. A comparison of these two methods showed that not all protein types could be detected on the 2D‐PAGE gels, and the dynamic range was somewhat limited. So the comprehensive proteome map from the iTRAQ analysis was used to identify the quantitatively regulated proteins that played roles in seed longevity, and found that the most marked change was the increased abundance of many metabolic enzymes, especially the ones involved in α‐linolenic acid metabolism in the embryos during ageing. OsLOX2 and OsLOX3 had a negative effect on seed longevity, which were lower in FH7185 than the control. The dehydrogenase (LOC_Os07g44430) which played a major catalytic role in lipid peroxidation, also changed significantly during ageing. Therefore, OsLOX2, OsLOX3 and Loc_OS 07G44430 may be involved in the regulation of seed longevity with a synergistic effect.

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Physiological and Biological Responses of Ca2+-Primed Quinoa Seed Longevity Stored at Different Hermetic Storage Conditions

Mamedi,

Sharifzadeh,

Maali-Amiri

et al. 2024

J Plant Growth Regul

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Mitochondrial structural and antioxidant system responses to aging in oat (Avena sativa L.) seeds with different moisture contents

Cited by 45 publications

References 35 publications

Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds

Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds

Comparative analysis of different methods revealing the involvement of proteins during ageing of rice seeds

Physiological and Biological Responses of Ca2+-Primed Quinoa Seed Longevity Stored at Different Hermetic Storage Conditions

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