Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds

Xia, Fangshan; Cheng, Huang; Chen, Lingling; Zhu, Huisen; Mao, Peisheng; Wang, Mingya

doi:10.1186/s12870-020-2321-x

Cited by 40 publications

(35 citation statements)

References 55 publications

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“…The mechanism through which priming treatments improve seed viability is related to the repair of cellular and mitochondrial components [ 11 ], synthesis of nucleic acids and proteins, and recovery of antioxidants [ 12 ]. Previous studies indicated that priming with exogenous substances, e.g., ascorbic acid (AsA), glutathione (GSH), and salicylic acid (SA), significantly accelerated the germination of aged seeds in many species, including Siberian wildrye ( Elymus sibiricus L.) [ 11 ], oat [ 13 ], and soybean ( Glycine max (L.) Merr.) [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Time-Course Physiological Responses and Proteomic Analysis of Melatonin Priming on Promoting Germination in Aged Oat (Avena sativa L.) Seeds

Yan

Mao

2021

IJMS

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Melatonin priming is an effective strategy to improve the germination of aged oat (Avena sativa L.) seeds, but the mechanism involved in its time-course responses has remained largely unknown. In the present study, the phenotypic differences, ultrastructural changes, physiological characteristics, and proteomic profiles were examined in aged and melatonin-primed seed (with 10 μM melatonin treatment for 12, 24, and 36 h). Thus, 36 h priming (T36) had a better remediation effect on aged seeds, reflecting in the improved germinability and seedlings, relatively intact cell ultrastructures, and enhanced antioxidant capacity. Proteomic analysis revealed 201 differentially abundant proteins between aged and T36 seeds, of which 96 were up-accumulated. In melatonin-primed seeds, the restoration of membrane integrity by improved antioxidant capacity, which was affected by the stimulation of jasmonic acid synthesis via up-accumulation of 12-oxo-phytodienoic acid reductase, might be a candidate mechanism. Moreover, the relatively intact ultrastructures enabled amino acid metabolism and phenylpropanoid biosynthesis, which were closely associated with energy generation through intermediates of pyruvate, phosphoenolpyruvate, fumarate, and α-ketoglutarate, thus providing energy, active amino acids, and secondary metabolites necessary for germination improvement of aged seeds. These findings clarify the time-course related pathways associated with melatonin priming on promoting the germination of aged oat seeds.

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Section: Introductionmentioning

confidence: 99%

Comparative Time-Course Physiological Responses and Proteomic Analysis of Melatonin Priming on Promoting Germination in Aged Oat (Avena sativa L.) Seeds

Yan

Mao

2021

IJMS

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“…Seed aging is an irreversible and natural process in which the vigor of seeds declines or loses completely. Seed aging has been a popular issue in seed research, as aged seeds lower seed emergence and growth, reduce overall germination performance and limit seed production [ 1 , 2 , 3 ]. Therefore, it is of great significance to distinguish aged seeds for ensuring seed quality and reducing economic losses.…”

Section: Introductionmentioning

confidence: 99%

Non-Destructive Identification of Naturally Aged Alfalfa Seeds via Multispectral Imaging Analysis

Wang

Zhang

Song

et al. 2021

Sensors

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Seed aging detection and viable seed prediction are of great significance in alfalfa seed production, but traditional methods are disposable and destructive. Therefore, the establishment of a rapid and non-destructive seed screening method is necessary in seed industry and research. In this study, we used multispectral imaging technology to collect morphological features and spectral traits of aging alfalfa seeds with different storage years. Then, we employed five multivariate analysis methods, i.e., principal component analysis (PCA), linear discrimination analysis (LDA), support vector machines (SVM), random forest (RF) and normalized canonical discriminant analysis (nCDA) to predict aged and viable seeds. The results revealed that the mean light reflectance was significantly different at 450~690 nm between non-aged and aged seeds. LDA model held high accuracy (99.8~100.0%) in distinguishing aged seeds from non-aged seeds, higher than those of SVM (87.4~99.3%) and RF (84.6~99.3%). Furthermore, dead seeds could be distinguished from the aged seeds, with accuracies of 69.7%, 72.0% and 97.6% in RF, SVM and LDA, respectively. The accuracy of nCDA in predicting the germination of aged seeds ranged from 75.0% to 100.0%. In summary, we described a nondestructive, rapid and high-throughput approach to screen aged seeds with various viabilities in alfalfa.

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“…The cycle is also involved in the regulation of plant growth and development, and the predominant role is assigned to glutathione, which has been linked to seed development [ 32 , 33 ], germination [ 20 , 34 , 35 ], and longevity [ 36 , 37 ]. Seed priming with AsA and/or GSH was found to successfully diminish aging damage in seeds [ 38 ]. Ascorbate (Asc) is less studied than its cycle partner, but its roles beyond being an antioxidant molecule have been documented [ 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

NAD(P)H Drives the Ascorbate–Glutathione Cycle and Abundance of Catalase in Developing Beech Seeds Differently in Embryonic Axes and Cotyledons

2021

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European beech is an important component of European lowland forests in terms of ecology, and produces irregular seeds categorized as intermediate due to their limited longevity. Removal of the excess of reactive oxygen species is crucial for redox homeostasis in growing plant tissues. Hydrogen peroxide (H2O2) is detoxified via the plant-specific ascorbate-glutathione cycle, and enzymatically, mainly by catalase (CAT). The reduced and oxidized (redox) forms of ascorbate (AsA, DHA) and glutathione (GSH, GSSG) decreased during maturation as the content of redox forms of nicotinamide adenine dinucleotide (NADH, NAD+) phosphate (NADPH, NADP+), cofactors of ascorbate–glutathione enzymes, declined and limited this cycle. The degree of oxidation of glutathione peaked at approximately 80%, at the exact time when the NADP content was the lowest and the NADPH/NADP+ ratio reached the highest values. The glutathione pool was reflected in changes in the NADP pool, both in embryonic axes (R2 = 0.61) and in cotyledons (R2 = 0.98). A large excess of NADPH was reported in embryonic axes, whereas cotyledons displayed more unified levels of NADP redox forms. As a result, anabolic redox charge and reducing power were higher in embryonic axes. CAT was recognized as two proteins, and the abundance of the 55 kDa protein was correlated with all redox forms of ascorbate, glutathione, NAD, and NADP, whereas the 37 kDa protein was oppositely regulated in embryonic axes and cotyledons. Here, we discuss the role of NAD(P) in the regulation of the ascorbate–glutathione cycle, catalase, and seed longevity concerning a putative role of NAD(P)H as a redox biomarker involved in predefining seed quality, because NAD(P)H-derived redox homeostasis was found to be better controlled in embryonic axes than cotyledons.

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Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds

Cited by 40 publications

References 55 publications

Comparative Time-Course Physiological Responses and Proteomic Analysis of Melatonin Priming on Promoting Germination in Aged Oat (Avena sativa L.) Seeds

Comparative Time-Course Physiological Responses and Proteomic Analysis of Melatonin Priming on Promoting Germination in Aged Oat (Avena sativa L.) Seeds

Non-Destructive Identification of Naturally Aged Alfalfa Seeds via Multispectral Imaging Analysis

NAD(P)H Drives the Ascorbate–Glutathione Cycle and Abundance of Catalase in Developing Beech Seeds Differently in Embryonic Axes and Cotyledons

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