Mitochondrial specific photodynamic therapy by rare-earth nanoparticles mediated near-infrared graphene quantum dots

Zhang, Dandan; Wen, Liewei; Huang, Ru; Wang, Huanhuan; Hu, Xianglong

doi:10.1016/j.biomaterials.2017.10.034

Cited by 209 publications

(146 citation statements)

References 73 publications

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“…Light exposure of both GQDs and MB can cause increased SO generation (5,6,11,43,44,46). We found previously that light exposure of the 1:1 GQD:MB preparation produced enhanced SO production compared to either MB or GQD alone in PBS (11).…”

Section: Singlet Oxygen Measurement and Imagingmentioning

confidence: 69%

“…Our previous work suggested that light exposure of combinations of graphene quantum dots with methylene blue caused eradication of both Gram-positive and Gram-negative bacteria (11). It seemed plausible to us that GQD-MB combinations could also be effective against cancer cells, which can be killed by SO and other ROS generated by graphene-based nanoparticles used in photodynamic therapy (6,9,20,43,44). We found that 24 h after light exposure, MCF-7 cell numbers were significantly reduced by the 1:1 GQD:MB preparation (8% of control; P ≤ 0.01), but that surprisingly, MB alone caused even lower cell survivability (4% of control; P ≤ 0.01) ( Fig.…”

Section: Cytotoxicity Experimentsmentioning

confidence: 99%

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Anticancer Photodynamic Therapy Properties of Sulfur‐Doped Graphene Quantum Dot and Methylene Blue Preparations in MCF‐7 Breast Cancer Cell Culture

Monroe

Belekov

et al. 2019

Photochem & Photobiology

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Photodynamic therapy (PDT) is a field with many applications including chemotherapy. Graphene quantum dots (GQDs) exhibit a variety of unique properties and can be used in PDT to generate singlet oxygen that destroys pathogenic bacteria and cancer cells. The PDT agent, methylene blue (MB), like GQDs, has been successfully exploited to destroy bacteria and cancer cells by increasing reactive oxygen species generation. Recently, combinations of GQDs and MB have been shown to destroy pathogenic bacteria via increased singlet oxygen generation. Here, we performed a spectrophotometric assay to detect and measure the uptake of GQDs, MB and several GQD‐MB combinations in MCF‐7 breast cancer cells. Then, we used a cell counting method to evaluate the cytotoxicity of GQDs, MB and a 1:1 GQD:MB preparation. Singlet oxygen generation in cells was then detected and measured using singlet oxygen sensor green. The dye, H2DCFDA, was used to measure reactive oxygen species production. We found that GQD and MB uptake into MCF‐7 cells occurred, but that MB, followed by 1:1 GQD:MB, caused superior cytotoxicity and singlet oxygen and reactive oxygen species generation. Our results suggest that methylene blue's effect against MCF‐7 cells is not potentiated by GQDs, either in light or dark conditions.

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Section: Singlet Oxygen Measurement and Imagingmentioning

confidence: 69%

Section: Cytotoxicity Experimentsmentioning

confidence: 99%

Anticancer Photodynamic Therapy Properties of Sulfur‐Doped Graphene Quantum Dot and Methylene Blue Preparations in MCF‐7 Breast Cancer Cell Culture

Monroe

Belekov

et al. 2019

Photochem & Photobiology

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“…The light-mediated cytotoxicity of GQDs, together with their energy-donor capacity, could therefore open a new area for GQD research in the life sciences -as direct theranostics or as cofactors or components of conventional photosensitizing agents used in PDT. Recent studies have investigated alternative syntheses and PDT-applicability of GQDs with encouraging results [25][26][27] .…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility and Toxicity of Graphene Quantum Dots for Potential Application in Photodynamic Therapy

Tabish

Scotton

Ferguson

et al. 2018

Nanomedicine (Lond.)

174

113

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“…[39][40] Mitochondria-targeting has attracted much attention for inducing mitochondriamediated apoptosis based on enhanced ROS generation, which can destroy the mitochondrial membrane, [41][42] resulting in effective therapeutic activity. [25,[43][44][45][46][47][48][49] Besides triphenylphosphonium and other lipophilic cations (ammonium), there are very few mitochondria-targeting agents. To the best of our knowledge, this is the first example of mitochondria-targeting in cells using WSONs of chlorin derivatives in PDT.…”

Section: Introductionmentioning

confidence: 99%

Mitochondria‐Targeted Water‐Soluble Organic Nanoparticles of Chlorin Derivatives for Biocompatible Photodynamic Therapy

Liu

Lee

et al. 2020

ChemNanoMat

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Photodynamic therapy (PDT) is a promising non‐invasive cancer therapy without any side‐effects. The photosensitizers (PSs) used in PDT are usually hydrophobic and not soluble in water. Water‐soluble PSs have been rarely studied in PDT research. Therefore, preparing water‐soluble PSs for clinical trials in PDT remains challenging. Here, we prepared water‐soluble organic nanoparticles (WSONs) using the following three chlorin derivatives: methyl pyropheophorbide a (MPPa), zinc metal‐introduced MPPa, and purpurin‐18 methyl ester. We used a very simple co‐precipitation method to develop WSONs through nanoprecipitation (solvent‐exchange) from THF to water. In this method, WSONs were developed in 87–112 nm sizes, based on TEM, with good water solubility and high water stability for more than a month. We evaluated intracellular accumulation and cell viability of these WSONs, exhibiting PDT results comparable to that of free PSs using HeLa cells. All WSONs were accumulated in mitochondria to induce mitochondria‐mediated apoptosis via effective PDT activity. This result is important not only for preparing WSONs with hydrophobic PSs to form highly water‐soluble PSs having good water stability in nanometre size, but also for retaining PDT activity with reduced dark toxicity, enabling potential applications for biocompatible PDT.

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Mitochondrial specific photodynamic therapy by rare-earth nanoparticles mediated near-infrared graphene quantum dots

Cited by 209 publications

References 73 publications

Anticancer Photodynamic Therapy Properties of Sulfur‐Doped Graphene Quantum Dot and Methylene Blue Preparations in MCF‐7 Breast Cancer Cell Culture

Anticancer Photodynamic Therapy Properties of Sulfur‐Doped Graphene Quantum Dot and Methylene Blue Preparations in MCF‐7 Breast Cancer Cell Culture

Biocompatibility and Toxicity of Graphene Quantum Dots for Potential Application in Photodynamic Therapy

Mitochondria‐Targeted Water‐Soluble Organic Nanoparticles of Chlorin Derivatives for Biocompatible Photodynamic Therapy

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