Mitochondrial-Mediated Apoptosis in Lymphoma Cells by the Diterpenoid Lactone Andrographolide, the Active Component of <i>Andrographis paniculata</i>

Yang, Shuo; Evens, Andrew M.; Prachand, Sheila; Singh, Amareshwar T.K.; Bhalla, Savita; David, Kevin A.; Li, Gordon

doi:10.1158/1078-0432.ccr-10-0883

Cited by 91 publications

(61 citation statements)

References 38 publications

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“…3). Second, blood from a human volunteer was collected, and lymphocytes were isolated using a Ficoll gradient (Materials and Methods) (17). Normal human lymphocytes did not undergo apoptosis when treated for 72 h with increasing doses of HDL-NP (Fig.…”

Section: Resultsmentioning

confidence: 99%

Biomimetic, synthetic HDL nanostructures for lymphoma

Yang

Damiano

Zhang

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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113

151

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New therapies that challenge existing paradigms are needed for the treatment of cancer. We report a nanoparticle-enabled therapeutic approach to B-cell lymphoma using synthetic high density lipoprotein nanoparticles (HDL-NPs). HDL-NPs are synthesized using a gold nanoparticle template to control conjugate size and ensure a spherical shape. Like natural HDLs, biomimetic HDL-NPs target scavenger receptor type B-1, a high-affinity HDL receptor expressed by lymphoma cells. Functionally, compared with natural HDL, the gold NP template enables differential manipulation of cellular cholesterol flux in lymphoma cells, promoting cellular cholesterol efflux and limiting cholesterol delivery. This combination of scavenger receptor type B-1 binding and relative cholesterol starvation selectively induces apoptosis. HDL-NP treatment of mice bearing B-cell lymphoma xenografts selectively inhibits B-cell lymphoma growth. As such, HDL-NPs are biofunctional therapeutic agents, whose mechanism of action is enabled by the presence of a synthetic nanotemplate. HDL-NPs are active in B-cell lymphomas and potentially, other malignancies or diseases of pathologic cholesterol accumulation.nanotechnology | therapy | biologic

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Section: Resultsmentioning

confidence: 99%

Biomimetic, synthetic HDL nanostructures for lymphoma

Yang

Damiano

Zhang

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

113

151

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“…8 The active principle, andrographolide is also attributed with some other activities like liver protection 9 , anticancer 10 and antimalarial activities. 11 Beside these, A. paniculata shows a broad range of pharmacological effects such as inhibition of replication of the HIV virus, antibacterial activity, antihyperglycemic effects, suppression of various cancer cells, antiinflammatory properties 12 and many more diseases.…”

Section: Introductionmentioning

confidence: 99%

Identification and Quantification of Andrographolide from Andrographis paniculata (Burm. f.) Wall. ex Nees by RP-HPLC Method and Standardization of its Market Preparations

Sajeeb

Kumar

Halder

et al. 2015

Dhaka Univ. J. Pharm. Sci

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ABSTRACT:Andrographis paniculata (Burm. f.) Wall. ex Nees, commonly known as Kalmegh, is widely used as antimalarial drug in herbal and traditional systems. Andrographolide is the major triterpenoid present in the plant and responsible for its therapeutic activities. The identification and quantification of andrographolide were ascertained by various spectrophotometric and chromatographic analyses. The plant sample was extracted with methanol. The amorphous residue obtained from extraction was analyzed for identification of andrographolide by chemical method, TLC, UV-Vis, FT-IR and LCMS/MS analyses. The quantitative estimation of andrographolide content in plant sample was carried out by simple reversed phase HPLC method with C 18 column using a mixture of water and methanol (35:65) as mobile phase at a flow rate of 0.7 mL/min, and the estimated concentration level of andrographolide was found as 38.36±0.42 µg/mL in the solution of amorphous residue (50 µg/mL). After quantitative determination of andrographolide, standardization of its market preparations was accomplished using the same RP-HPLC method. The estimated % potencies of andrographolide compared to reference standard in six market preparations were found to be 97. 56, 98.37, 98.21, 95.60, 98.91 and 96.40.

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“…these cells through activation of caspases-3, -8, and -9 that was inhibited by an ROS scavenger (341). Dimethylaminoparthenolide, a water-soluble parthenolide analog, has also been shown to induce apoptosis in prostate cancer cells in vivo by targeting NF-jB and generating ROS (276).…”

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confidence: 99%

Upsides and Downsides of Reactive Oxygen Species for Cancer: The Roles of Reactive Oxygen Species in Tumorigenesis, Prevention, and Therapy

Gupta

Hevia

Patchva

et al. 2012

Antioxidants & Redox Signaling

607

464

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Significance: Extensive research during the last quarter century has revealed that reactive oxygen species (ROS) produced in the body, primarily by the mitochondria, play a major role in various cell-signaling pathways. Most risk factors associated with chronic diseases (e.g., cancer), such as stress, tobacco, environmental pollutants, radiation, viral infection, diet, and bacterial infection, interact with cells through the generation of ROS. Recent Advances: ROS, in turn, activate various transcription factors (e.g., nuclear factor kappa-light-chain-enhancer of activated B cells [NF-jB], activator protein-1, hypoxia-inducible factor-1a, and signal transducer and activator of transcription 3), resulting in the expression of proteins that control inflammation, cellular transformation, tumor cell survival, tumor cell proliferation and invasion, angiogenesis, and metastasis. Paradoxically, ROS also control the expression of various tumor suppressor genes (p53, Rb, and PTEN). Similarly, c-radiation and various chemotherapeutic agents used to treat cancer mediate their effects through the production of ROS. Interestingly, ROS have also been implicated in the chemopreventive and anti-tumor action of nutraceuticals derived from fruits, vegetables, spices, and other natural products used in traditional medicine. Critical Issues: These statements suggest both ''upside'' (cancer-suppressing) and ''downside'' (cancer-promoting) actions of the ROS. Thus, similar to tumor necrosis factor-a, inflammation, and NF-jB, ROS act as a double-edged sword. This paradox provides a great challenge for researchers whose aim is to exploit ROS stress for the development of cancer therapies. Future Directions: The various mechanisms by which ROS mediate paradoxical effects are discussed in this article. The outstanding questions and future directions raised by our current understanding are discussed. Antioxid. Redox Signal. 16, 1295Signal. 16, -1322

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Mitochondrial-Mediated Apoptosis in Lymphoma Cells by the Diterpenoid Lactone Andrographolide, the Active Component of Andrographis paniculata

Cited by 91 publications

References 38 publications

Biomimetic, synthetic HDL nanostructures for lymphoma

Biomimetic, synthetic HDL nanostructures for lymphoma

Identification and Quantification of Andrographolide from Andrographis paniculata (Burm. f.) Wall. ex Nees by RP-HPLC Method and Standardization of its Market Preparations

Upsides and Downsides of Reactive Oxygen Species for Cancer: The Roles of Reactive Oxygen Species in Tumorigenesis, Prevention, and Therapy

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