Biomimetic, synthetic HDL nanostructures for lymphoma

Abstract

New therapies that challenge existing paradigms are needed for the treatment of cancer. We report a nanoparticle-enabled therapeutic approach to B-cell lymphoma using synthetic high density lipoprotein nanoparticles (HDL-NPs). HDL-NPs are synthesized using a gold nanoparticle template to control conjugate size and ensure a spherical shape. Like natural HDLs, biomimetic HDL-NPs target scavenger receptor type B-1, a high-affinity HDL receptor expressed by lymphoma cells. Functionally, compared with natural HDL, the gold NP template enables differential manipulation of cellular cholesterol flux in lymphoma cells, promoting cellular cholesterol efflux and limiting cholesterol delivery. This combination of scavenger receptor type B-1 binding and relative cholesterol starvation selectively induces apoptosis. HDL-NP treatment of mice bearing B-cell lymphoma xenografts selectively inhibits B-cell lymphoma growth. As such, HDL-NPs are biofunctional therapeutic agents, whose mechanism of action is enabled by the presence of a synthetic nanotemplate. HDL-NPs are active in B-cell lymphomas and potentially, other malignancies or diseases of pathologic cholesterol accumulation.nanotechnology | therapy | biologic

The Cardioprotective Protein Apolipoprotein A1 Promotes Potent Anti-tumorigenic Effects

“…Synthetic HDL nanoparticles were recently shown to inhibit B cell lymphoma xenografts in mice in a scavenger receptor class B, type I-dependent manner (26). Interestingly, these authors did not observe any anti-tumor effect by HDL from human serum.…”

Green Synthesis and Characterization of Gold Nanoparticles: Study of Its Biological Mechanism in Human SUDHL-4 Cell Line

“…HAuCl 4 alone caused a drop in survival of 20%. At the same concentration (200 μM), AuNPs caused a dramatic drop in cell viability of more than 84% (Figure 5(a)) [40] [42].…”

Nanotechnologies

“…High density lipoprotein nanoparticles (HDL-NPs) are synthesized using a gold nanoparticle template to control conjugate size and ensure a spherical shape (Yang et al 2013). Like natural HDLs, biomimetic HDL-NPs target scavenger receptor type B-1, a high-affinity HDL receptor expressed by lymphoma cells.…”