Mitochondrial lineages and DNA barcoding of closely related species in the mayfly genus Ephemerella (Ephemeroptera:Ephemerellidae)

DNA barcoding to confirm morphological traits and determine relative abundance of burrowing mayfly species in western Lake Erie

“…DNA barcoding analysis is dependent on the assumption of a significant gap between intraspecific and interspecific DNA variation (Meyer and Paulay, 2005), especially for identifying cryptic species. Previous results of barcoding analysis have been inconclusive if investigators encountered considerable overlap between intra-and interspecific variation (Alexander et al, 2009;Meyer and Paulay, 2005). Our results confirm the existence of a significant gap between H. limbata and H. rigidia COI sequences and support our hypothesis that DNA barcoding can be used to distinguish these cryptic species.…”

“…Mismatch distributions, which display differences in nucleotide sequences among pairs of individuals, were used to visualize the genetic differences within and between Hexagenia species and determine whether fixed nucleotide differences were present. If our hypothesis that DNA Barcoding will be useful for Hexagenia is correct, then mismatch analysis will yield a bi-modal distribution with a "Barcoding Gap" between species (Alexander et al, 2009;Meyer and Paulay, 2005). A Neighbor-joining tree was generated for a 609 bp fragment of the COI gene with an Approximate Likihood-Ratio Test (aLRT) (Phylogeny.fr; Dereeper et al, 2008).…”

Testing the Potential of Proposed DNA Barcoding Markers inNezara virudulaandNezara antennataWhen Geographic Variation and Closely Related Species Were Considered

“…The COI gene as the core of the global bio-identification system for animals has suffered great disputations DeSalle 2005;Hurst and Jiggins 2005;Meier et al 2006;Memon et al 2006, Jansen et al 2009Sundberg et al 2010;Yassin et al 2010). The most important problem is the observed wide overlap between intra-and interspecific sequence variability using COI as the molecular marker (Meyer and Paulay 2005;Meier et al 2006;Memon et al 2006;Alexander et al 2009;Jansen et al 2009). The lack of resolving power of the COI sequence has led researchers to envisage the primary COI-based method.…”

DNA-based association of adults and larvae in Baetidae (Ephemeroptera) with the description of a new genusAdnoptilumin Madagascar

“…One technique is to use a threshold value of sequence divergence for species discrimination (i.e., DNA barcoding), and this method has been used for mayflies (Ball et al 2005, Alexander et al 2009). The success of this approach has been mixed (Hebert et al 2004, Brower 2006, and possible explanations for its failure include nonuniform rates of sequence divergence and use of inaccurate a priori groups to calibrate a threshold of sequence divergence between species .…”

“…The success of this approach has been mixed (Hebert et al 2004, Brower 2006, and possible explanations for its failure include nonuniform rates of sequence divergence and use of inaccurate a priori groups to calibrate a threshold of sequence divergence between species . Reliance on a single gene marker, usually mitochondrial DNA (mtDNA), also can lead to inaccurate estimates of diversity because of introgression or incomplete lineage sorting (Pollard et al 2006, Alexander et al 2009. A 2 nd technique is characterbased and uses diagnostic genetic markers rather than similarity measures (Sites and Marshall 2003).…”