DNA barcoding to confirm morphological traits and determine relative abundance of burrowing mayfly species in western Lake Erie

Letter to the Editor

“…The protocols originally derived by Fremling [13] have been refined through extensive laboratory research at the University of Windsor (ON, Canada) [ [14][15][16][17][18][19][20]; E.C. Taxonomic keys are now available to reliably identify H. limbata and H. rigida larvae and adult females to the species level [22]. Hexagenia limbata and Hexagenia rigida coexist in the Detroit River, from which the eggs are collected annually in a manner similar to that described by Harwood et al [1,21].…”

“…Hexagenia limbata and Hexagenia rigida coexist in the Detroit River, from which the eggs are collected annually in a manner similar to that described by Harwood et al [1,21]. Taxonomic keys are now available to reliably identify H. limbata and H. rigida larvae and adult females to the species level [22]. Refrigerated eggs can be stored for 8 mo or longer [23].…”

DNA barcode assessment of Mediterranean mayflies (Ephemeroptera), benchmark data for a regional reference library for rapid biomonitoring of freshwaters

“…50% of the continental known Ephemeropterans. Further studies took advantage of DNA barcodes to clear systematic relationships within a family level (Sroka, 2012), to differentiate species with morphologically indistinguishable nymphs and adult females (Elderkin et al, 2012), for understanding the local population structure (Ogitani et al, 2011), to dissect cryptic species complexes (Williams et al, 2006;Ståhls and Savolainen, 2008), and for phylogeographic inferences on endemic taxa (Gattolliat et al, 2015).…”

Molecular phylogeny and DNA barcoding confirm cryptic species in the African freshwater oysterEtheria ellipticaLamarck, 1807 (Bivalvia: Etheriidae)

“…Previous studies have used this analysis to determine cryptic species, where a mismatch analysis will yield a bi-modal distribution with a 'Barcoding Gap' between species (Meyer & Paulay, 2005;Alexander et al, 2009;Elderkin et al, 2012;Pri e & Puillandre, 2014). Previous studies have used this analysis to determine cryptic species, where a mismatch analysis will yield a bi-modal distribution with a 'Barcoding Gap' between species (Meyer & Paulay, 2005;Alexander et al, 2009;Elderkin et al, 2012;Pri e & Puillandre, 2014).…”

“…Mismatch distributions, which display pairwise nucleotide differences, were used to visualize the genetic differences within and between Etheria samples and determine whether fixed nucleotide differences were present. Previous studies have used this analysis to determine cryptic species, where a mismatch analysis will yield a bi-modal distribution with a 'Barcoding Gap' between species (Meyer & Paulay, 2005;Alexander et al, 2009;Elderkin et al, 2012;Pri e & Puillandre, 2014). We also used ARLE-QUIN to calculate preliminary estimates of both genetic diversity and pairwise estimates of F ST within each clade.…”