Mitochondrial Haplotype Analysis of <i>Pomoxis nigromaculatus</i> Inhabiting Three Georgian Lakes

Abstract

Pomoxis nigromaculatus, more commonly referred to as black crappie is indigenous to fresh water streams and lakes in the eastern United States and supports an important recreational fishery. We examined the genetic population structure of black crappie inhabiting three Georgian Lakes, Lake Sidney Lanier, Lake Seminole and Hartwell Lake. DNA sequencing of 229 fish samples, utilizing the DNA barcode marker cytochrome oxidase subunit I (COI) revealed 27 polymorphic sites which defined nine haplotypes. Only haplotype 2 was shared between all sample sites with six other haplotypes being unique for individual lakes, for an overall haplotype diversity of 0.734. Tajima's D and Fu's tests were implemented to assess departures from neutral expectations. Fst pairwise comparisons were statistically significant among all populations of black crappie evaluated in this study.

DNA barcoding affirms the presence of invasiveParachanna obscuraand new records of some species in the Mweru-Luapula fishery

“…These challenges are exacerbated by a lack of key guides for most Southern African freshwater fishes. The possible mismatches of T. polylepis for T. mylodon, T. buysi for A. macrophthalmus, O. niloticus for O. macrochir; C. zillii for C. rendalli, M. kannume for M. longirostris and S. nigromaculata for S. katangae from field identification validates the observations made by Erwin & George [7] stressing the limitations of solely using visual identifications due to convergent morphological characteristics in some species. In line with Becker et al [78], a precautionary approach should be incorporated in addressing taxonomic mismatches arising from low quality sequences submitted to the GenBank database.…”

“…On the other hand, the mismatches observed can potentially be attributed to hybridization and introgression that would have led to morphological features being similar to one species but the DNA sequence of a different taxon [76,77]. [7] stressing the limitations of solely using visual identifications due to convergent morphological characteristics in some species. In line with Becker et al [78], a precautionary approach should be incorporated in addressing taxonomic mismatches arising from low quality sequences submitted to the GenBank database.…”

“…DNA barcoding is a fast-growing tool that has been incorporated in the management and monitoring of various ecosystems across the globe [1,3,4). The technique is robust, reliable and the most accurate method of fish identification [5] down to the species level [6], and can also be applied in population delineation studies [7]. DNA barcodes provide a reference database to detect and identify invasive species within impacted communities [8].…”

Molecular phylogeny of anchovy (Clupeiformes: Clupeidae) from southern waters of Lombok using mitochondrial DNA CO1 gene sequences

“…The results evaluation of departures from neutral expectations using Tajima's D and Fu's showed that first pairwise comparisons were statistically significant among all populations of S. delicatulus evaluated in this study. According to Erwin and George (2017), the inhabitants of Spratelloides in this study have low to moderate haplotype diversity may affect their ability to respond to a rapidly changing environment, especially when coupled with increased fishing pressure. This species is not highly migratory, and with increased gene flow through the removal of artificial fences, an efficient fish passage may allow for the buildup of genetic disparity.…”