Mitochondrial Dysfunction and Apoptosis in Cumulus Cells of Diabetic Mice.

Wang, Qiang; Frolova, Antonina I.; Boehle, Katie L.; Schoeller, Erica L.; Chi, Maggie; Schedl, Tim; Moley, Kelle H.

doi:10.1093/biolreprod/83.s1.633

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“…Ziegler & Groscurth 63 reported that, in the cytosol, cytochrome C activated pro-caspase 9, 3, 6, and 7. These caspases led to apoptosis in the cumulus cells 64 . In addition, a previous study reported that 10-gingerol is a hydrophobic compound 19 and possesses anti-carcinogenic properties that are better than other gingerols 25 .…”

Section: Discussionmentioning

confidence: 95%

10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies

Kiptiyah

Widodo

Ciptadi

et al. 2017

Journal of Taibah University Medical Sciences

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Objectives Cumulus cells play a crucial role as essential mediators in the maturation of ova. Ginger contains 10-gingerol, which induces apoptosis in colon cancer cells. Based on this hypothesis, this study aimed to determine whether 10-gingerol is able to induce apoptosis in normal cells, namely, cumulus cells. Methods This study used an in vitro analysis by culturing Cumulus cells in M199 containing 10-gingerol in various concentrations (12, 16, and 20 μM) and later detected early apoptotic activity using an Annexin V-FITC detection kit. Result The in vitro data revealed that the number of apoptosis cells increased along with the period of incubation as follows: 12 μM (63.71% ± 2.192%); 16 μM (74.51% ± 4.596%); and 20 μM (78.795% ± 1.435%). The substance 10-gingerol induces apoptosis in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT-1. Conclusions These findings indicate that further examination is warranted for 10-gingerol as a contraception agent.

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