Mitochondrial Dna Variation among Muscidifurax spp. (Hymenoptera: Pteromalidae), Pupal Parasitoids of Filth Flies (Diptera)

Taylor, David B.; Peterson, Richard D.; Szalanski, Allen L.; Petersen, J. J.

doi:10.1093/aesa/90.6.814

Cited by 36 publications

(25 citation statements)

References 26 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This tends to agree with the results of mitochondrial DNA [8]. Neither the diet of parasitoids nor seasonal effects are expected to significantly alter the observed CHCs.…”

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

Gas chromatography/mass spectrometry analysis of the cuticular hydrocarbons from parasitic wasps of the genus Muscidifurax

Bernier¹,

Carlson²,

Geden³

1998

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

“…This tends to agree with the results of mitochondrial DNA [8]. Neither the diet of parasitoids nor seasonal effects are expected to significantly alter the observed CHCs.…”

Section: Discussionsupporting

confidence: 87%

“…Techniques such as RAPD-PCR [7] and mitochondrial DNA [8] have been successfully used to differentiate these closely related species. However, differentiation of M. uniraptor and M. raptorellus was difficult using mitochondrial DNA.…”

mentioning

confidence: 99%

Gas chromatography/mass spectrometry analysis of the cuticular hydrocarbons from parasitic wasps of the genus Muscidifurax

Bernier¹,

Carlson²,

Geden³

1998

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

“…MseI was also used to digest some of the smaller mtDNA amplicons. Digest products were visualized by separation with seakem or metaphor agarose (FMC) or polyacrylamide gel electrophoresis and stained with ethidium bromide (Taylor et al, 1997). The number of base pairs surveyed with each mtDNA amplicon was estimated using the formula (number of restriction fragments -1) × (length of the recognition sequence for each restriction enzyme).…”

Section: Methodsmentioning

confidence: 99%

Genetic variation in geographical populations of western and Mexican corn rootworm

Szalanski

Roehrdanz

Taylor

et al. 1999

Insect Molecular Biology

View full text Add to dashboard Cite

Genetic variation in the nuclear rDNA ITS1 region of western corn rootworm, Diabrotica virgifera virgifera (WCR), and Mexican corn rootworm, D. v. zeae (MCR) was studied. Two sites were detected which differentiated WCR and MCR in the 642-base sequence. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the first internal transcribed spacer region (ITS1) sequence revealed no variation within or among the twelve WCR and two MCR populations. PCR-RFLP of 75% of the mitochondrial DNA genome detected one significant polymorphic site out of the approximately 190 restriction sizes observed in WCR. The polymorphism did not differentiate geographical populations of WCR and is not diagnostic for the subspecies. The low levels of variation observed in WCR suggests either high levels of gene flow or a recent geographical expansion from a relatively small base. Gene flow would facilitate the rapid spread of traits that could compromise control programmes, such as insecticide resistance or behavioural modifications. The minimal genetic differentiation between WCR and MCR raises questions about the evolutionary history of these subspecies and how the distinct phenotypes are maintained.

show abstract

“…PCR reaction mix consisted of 5 l 10ϫ PCR Buffer II (Perkin Elmer), 4 l 25 mM Mg Cl, 1 l each of 10 mM nucleotides, 0.5 l Amplitaq Gold polymerase (Perkin Elmer), 0.5 l each of 100 M primers, and sterile water to volume. The primers used included: C1Ð2797 (C1-J-2797, 5Ј-CCTCGACGTTATTCAGAT-TACC-3Ј, Simon et al 1994); C2-3380 (C2-N-3380, 5Ј-TCAATATCATTGATGACCAAT-3Ј, Taylor et al 1997); WCR-137 (C1-N-2950, 5Ј-AGCTGGAGGGT-TAAATTGTAG-3Ј); WCR-158 (C1-J-2991, 5Ј-GAA-CACAGATATTCTGAAGTG-3Ј); NCR-410 (C2-J-3250, 5Ј-TTTTTGCACCGAAATCTATTA-3Ј), and NCR-415 (C2-N-3231, 5Ј-CCTTCTAATAGATTTCG-GTGC-3Ј). The four-digit numbers demark the 3Ј end of the primers in the D. yakuba mtDNA sequence (Clary and Wolstenholme 1985).…”

Section: Methodsmentioning

confidence: 99%

Multiplex Polymerase Chain Reaction Method for Differentiating Western and Northern Corn Rootworm Larvae (Coleoptera: Chrysomelidae)

Roehrdanz

2003

View full text Add to dashboard Cite

Western corn rootworm, Diabrotica virgifera virgifera LeConte, and northern corn rootworm, D. barberi Smith and Lawrence, are sympatric species and serious pests of corn cultivation in North America. Comparison of nucleotide sequence of mitochondrial cytochrome oxidase I and II was used to design polymerase chain reaction (PCR) primers that discriminate immature stages of the two species based on differences in amplicon size. Multiplex PCR can be used to give a positive test for each species in a single amplification reaction. This provides a method to identify field caught larvae and facilitates investigations of larval interaction and competition between the species.

show abstract

Mitochondrial Dna Variation among Muscidifurax spp. (Hymenoptera: Pteromalidae), Pupal Parasitoids of Filth Flies (Diptera)

Cited by 36 publications

References 26 publications

Gas chromatography/mass spectrometry analysis of the cuticular hydrocarbons from parasitic wasps of the genus Muscidifurax

Gas chromatography/mass spectrometry analysis of the cuticular hydrocarbons from parasitic wasps of the genus Muscidifurax

Genetic variation in geographical populations of western and Mexican corn rootworm

Multiplex Polymerase Chain Reaction Method for Differentiating Western and Northern Corn Rootworm Larvae (Coleoptera: Chrysomelidae)

Contact Info

Product

Resources

About