Natural and synthetic long-chain alkenes, alkadienes, and alkatrienes (C25-C37) were readily derlvatlzed to stable adducts with dimethyl disulfide (DMDS). This technique was extended to mixtures of unseparated alkanes, alkenes, alkadienes, and alkatrienes, but limited here to the types found In two-winged Insects Including African honey bees, fruit flies, and stable flies, which included several unreported compounds. Capillary column gas chromatography/electron Ionization mass spectrometry (GC/EI-MS) of stable DMDS adducts allowed location of both Internal and terminal unsaturated sites. Derivatization of the total hydrocarbon fraction gave excellent results after only 4 h of reaction time. Alkanes eluted first, followed by the alkene adducts, then the dladducts of dienes, with each group well separated from the others. Molecular ions of diand trladducts were observed, together with Ions showing losses of 48, 94, 141, and 188; cleavage at carbons bearing SCH3, then losses of 48 and 94 located both double bonds. The Cto and C37 dladducts were well separated during GC/MS or GC, with no thermal cleavage observed. Homologous C^, C24, and C2S trlene dladducts and the C2S trlene triadduct survived GC/MS and displayed characteristic cleavage patterns. Major differences were found in proportions of honey bee alkenes that differentiated European and African races, as the C31, C33, and C& alkenes, mentioned as diagnostic for the races, were present In the diagnostic proportion when viewed as adducts. Also, a 9-Isomer in the C31 homologue Indicated the presence of African genetic material In worker bees and wax. The variety of dienes giving linear adducts was limited In that the double bonds were separated by four or more methylenes; otherwise cyclic derivatives of up to 29 carbons were observed.