Mitochondrial DNA variability in natural populations of Hawaiian Drosophila. I. Methods and levels of variability in D. silvestris and D. heteroneura populations

DeSalle, Rob; Giddings, Luther Val; Templeton, Alan R.

doi:10.1038/hdy.1986.11

Cited by 49 publications

(17 citation statements)

References 44 publications

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“…Sequence divergences between H. irritans and H. exigua (1.8ῌ1.9ῌ) in the present study are larger than the range of the estimated values for intraspecies di#eren-tiation based on mtDNA in various insect species which have been reported by DeSalle et al (1986), Martin and Simon (1990), and Vargas and Espin (1995). mtDNA analysis of haplotypes of the calliphorid flies Chrysomya albiceps and C. rufifacies (which are closely related to each other) showed 0.04ῌ0.8ῌ intraspecies di#erences and 2.9ῌ3.1ῌ interspecies di#erences (Wells and Sperling, 1999).…”

Section: D>h8jhh>dccontrasting

confidence: 50%

Genetic and morphological differences of Haematobia irritans and H. exigua, and molecular phylogeny of Japanese Stomoxyini flies (Diptera, Muscidae)

Iwasa

Ishiguro

2010

Jap. J. Sanit. Zool.

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Molecular analysis based on mitochondrial DNA (mtDNA) sequence was performed to elucidate the genetic di#erence between Haematobia irritans (Linnaeus) and H. exigua (de Meijere), and the phylogenetic relationship among Japanese Stomoxyini species (Stomoxys calcitrans (Linnaeus), S. indicus Picard, S. uruma Shinonaga et Kano, and Haematobosca sanguinolenta (Austen) including Haematobia). Two populations obtained from Obihiro and Morioka which were morphologically identified as H. irritans were genetically identical. Two populations obtained from Taiwan and Vietnam which were morphologically identified as H. exigua were almost genetically identical, but there was a small divergence (0.3῍0.4῎) between them. Nucleotide sequence divergences between H. irritans from Obihiro-Morioka and H. exigua from Taiwan-Vietnam were 1.8῍1.9῎, and those between Haematobia and other separate species of Stomoxys and Haematobosca were 6.7῍10.9῎. In cluster analysis based on mtDNA, Haematobia species were close to Haematobosca sanguinolenta. S. calcitrans and S. uruma were closely related to each other, and S. indicus was distantly related to these two species. We discussed the status of H. irritans and H. exigua based on mtDNA, and the discrepancies of molecular phylogeny versus the previous hypotheses based on morphological criteria in Japanese Stomoxyini.

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Section: D>h8jhh>dccontrasting

confidence: 50%

Genetic and morphological differences of Haematobia irritans and H. exigua, and molecular phylogeny of Japanese Stomoxyini flies (Diptera, Muscidae)

Iwasa

Ishiguro

2010

Jap. J. Sanit. Zool.

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“…It appears from mtDNA restriction sites that hybridisation of D. silvestris and D. heteroneura may have occurred on the west side of Hawaii (DeSalle et a!., 1985). In accordance with the mtDNA data, suspected hybrids of these two species have indeed been taken in the field at a west side collecting site (Kaneshiro and Val, 1977).…”

Section: S3s2s1 H5116h7s8s3 L14s253supporting

confidence: 54%

“…No fixed isozyme variants nor fixed chromosomal inversions have been found (Carson, 1983). Hunt and Carson (1982) have shown that a small but significant amount of single copy nuclear DNA sequence variation exists (0.6 per cent) and we (DeSalle et a!., 1985) have shown the existence of substantial mtDNA restriction site variability. Restriction site analysis of mtDNA may hence be useful in the genetic analysis of these species.…”

Section: Introductionmentioning

confidence: 98%

Mitochondrial DNA variability in natural populations of Hawaiian Drosophila. II. Genetic and phylogenetic relationships of natural populations of D. silvestris and D. heteroneura

DeSalle

1986

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Restriction site mapping of mitochondrial DNA with 23 restriction endonucleases was used to examine the genetic and phylogenetic relationships of populations of D. silvestris and D. heteroneura from the island of Hawaii. Two morphological races of D. silvestris are known on the island of Hawaii. One has three bristle rows on the tibia of the foreleg and is found on the east side of the island. The other is found on the west side of the island and has the ancestral bristle row character of two rows on the tibia of the foreleg. All D. heteroneura have the ancestral bristle row character state. We demonstrate that mtDNA restriction site analysis can also differentiate the two D. silvestris races, and that the two bristle row D. silvestris are more closely related to D. heteroneura than they are to their three bristel row conspecifics using both distance and character state analysis. Our study (which uses six base recognition restriction endonucleases) is not sensitive enough to determine the phylogenetic relationships of populations within either of the D. silvestris lineages.

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“…DNA manipulation and restriction site mapping were as described (18). The (16) or are available on request from the authors.…”

Section: Methodsmentioning

confidence: 99%

Discordance of nuclear and mitochondrial DNA phylogenies in Hawaiian Drosophila.

DeSalle¹,

Giddings²

1986

Proc. Natl. Acad. Sci. U.S.A.

105

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Drosophila differens, endemic to Molokai, Drosophila planitibia of Maui, and Drosophila silvestris and Drosophila heteroneura from the island of Hawaii are chromosomally homosequential species that presumably have colonized the newer islands of the Hawaiian archipelago by sequential founder events. We have examined the phylogenetic relationships of these four species by using mitochondrial DNA restriction site data for 23 enzymes. Both distance and character-state analyses indicate that a sequential or monotonic branching relationship exists for mtDNA restriction site data from the four species. The mtDNA data suggest that the maternal lineage that gave rise to D. differens is ancestral to the D. planitibia maternal lineage, which in turn shares the most recent common ancestor with the D. silvestris and D. heteroneura maternal lineages [with Drosophila hemipeza (Oahu) and Drosophila neopicta (Molokai and Maui) as outside references]. We also discuss the phylogenetic implications of the mtDNA data in comparison with other sources of phylogenetic data. We conclude that hybridization of the species in this group has been an important factor in the evolution of the nuclear genomes. Because of small population sizes and mating asymmetries, it is possible that the nuclear genetic distance of species that are physically capable of hybridizing (e.g., on the same island or island complex) is depressed. Consequently the mtDNA genetic distance appears to be more sensitive in establishing the sequence of evolutionary events responsible for the present distribution and population structure of these species. Molokai is thought to be the center of the planitibia subgroup radiation (5). These four species are differentiated chromosomally from other of the planitibia subgroup flies by having a unique polytene inversion designated "Xr" (1, 5-7).Several classical evolutionary approaches (discussed below) have been taken to examine the phylogeny of these four closely related species. In particular, the identification of the putative founders of D. heteroneura and D. silvestris has been attempted (1,3,(8)(9)(10). A novel, possibly more sensitive approach to the analysis of these organisms is the examination of DNA sequences with restriction endonucleases. The development of statistical tests for the analysis of phylogenies based on restriction sites and DNA sequence data (11-13) also makes this approach attractive.For a number of reasons, mtDNA appears to be a suitable choice to examine the phylogeny of these organisms. First, mtDNA evolves at a rate suited to the study of groups whose divergence times are no greater than from 8 to 10 Myr (14). The four species in this study are most likely to have diverged within the last 2 Myr (15). Second, the sensitivity of certain DNA sequences to stochastic changes should also be considered. Much of the evolution of this subgroup is thought to have occurred via founder/flush cycles, with the founders being perhaps so few as single gravid females. Therefore, a maternal marker might be s...

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Mitochondrial DNA variability in natural populations of Hawaiian Drosophila. I. Methods and levels of variability in D. silvestris and D. heteroneura populations

Cited by 49 publications

References 44 publications

Genetic and morphological differences of Haematobia irritans and H. exigua, and molecular phylogeny of Japanese Stomoxyini flies (Diptera, Muscidae)

Genetic and morphological differences of Haematobia irritans and H. exigua, and molecular phylogeny of Japanese Stomoxyini flies (Diptera, Muscidae)

Mitochondrial DNA variability in natural populations of Hawaiian Drosophila. II. Genetic and phylogenetic relationships of natural populations of D. silvestris and D. heteroneura

Discordance of nuclear and mitochondrial DNA phylogenies in Hawaiian Drosophila.

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