Mitochondrial DNA reveals species composition and phylogenetic relationships of hookworms in northeastern Brazil

Egg genotyping reveals the possibility of patent Ancylostoma caninum infection in human intestine

“…In fact, according to Loukas and coworkers 5 , N. americanus is the predominant human hookworm, which globally accounts for the majority of patent human hookworm cases and is especially common in the Americas. These data corroborate the molecular analyses of Monteiro and coworkers 25 , who evaluated hookworm populations in humans in northeastern Brazil. Brooker and coworkers 26 , by using morphological analysis, observed that all adult hookworms recovered from populations in southeastern Brazil were N. americanus, while Marzochi and Chieffi 27 , by using the morphological analysis of larvae obtained after fecal culture, observed the presence of A. duodenale in 14.2% of the analyzed samples.…”

“…The lack of fecal DNA amplification in lanes 2 and 5 indicates there was no contamination with animal fecal samples. fragment length polymorphism (RFLP-PCR) 12,20 , high resolution melting (HRM) 21 , quantitative real-time multiplex PCR 22 , single-strand conformation polymorphism (SSCP) 23 and sequencing 24,25 . All of these studies performed analyses of adult worms and egg/larvae pools.…”

Morphologic Characterization of Strongylida Larvae from Human and Swine Coprocultures in Rural Communities in the State of Piauí, Northeastern Brazil

“…In previous works, we demonstrated that human hookworm disease is endemic in the studied area and that the species involved in the vast majority of cases was N. americanus , through DNA sequencing of the parasite's cytochrome oxidase 1 coding gene ( cox 1) fragment [ 15 , 25 ]. Nevertheless, in some hookworm-positive fecal samples, it was not possible to characterize the Necator species present in the material [ 15 ]. Previous reports on swine hookworms also motivated the investigation of the presence of Necator in pigs in the region [ 14 , 26 – 29 ].…”

“…Necator suillus is a hookworm species described in pigs for almost a century [14], and investigations into its validity, occurrence, epidemiology, and zoonotic potential have not been forwarded. e present work is part of a broader project, in which we assessed, through molecular tools (mitochondrial DNA sequencing), the zoonotic circulation of Strongylida and Ascaris in the human-swine interface in low-resource rural communities in the state of Piauí [15][16][17][18]. In this report, we present the results of the morphological study of Strongylida larvae obtained through cultivation of human and swine feces.…”

“…The present work is part of a broader project, in which we assessed, through molecular tools (mitochondrial DNA sequencing), the zoonotic circulation of Strongylida and Ascaris in the human-swine interface in low-resource rural communities in the state of Piauí [ 15 – 18 ]. In this report, we present the results of the morphological study of Strongylida larvae obtained through cultivation of human and swine feces.…”