Mitochondrial DNA from Oenothera berteriana

Abstract

Mitochondrial DNA (mt-DNA) from Oenothera berteriana tissue culture cells was isolated and characterized with respect to buoyant density in CsCI, melting point, contour length, and restriction fragments.Because of the rather long purification procedure very few molecules retained their circularity. Only one distinct size class of molecules with a length of 100 kilobases was found. Restriction So far, mt-DNA2 from higher plants has been studied with only a limited choice of methods. Buoyant density in CsCl has been determined to be generally 1.706 g/cm3 (16,17,25). Kinetic complexity of mt-DNA of peas as determined by reassociation curves was with 70 x 106 daltons in agreement with the electronmicroscopy of molecules from this species in which a single size class of 35 lsm was observed. These data do not seem valid for the whole plant kingdom. In some species such as maize (16)

Transcripts of the NADH-dehydrogenase subunit 3 gene are differentially edited in Oenothera mitochondria.

“…Tissue culture Oenothera tissue culture cells were grown as callus cultures on solid agar medium in the dark as previously described and were harvested after 3 weeks of subculture (Brennicke, 1980).…”

Mitochondrial genome of Thymus vulgaris L. (Labiate) is highly polymorphic between and among natural populations

“…Chloroplast and mitochondrial DNAs are considered to be maternally transmitted among Angiosperms. However, important exceptions have been reported -Pelargonium (Tilney- Basset, 1978), Oenothera (Brennicke, 1980), Medicago sativa (Schumann & Hancock, 1989), Brassica napus (Erickson & Kemble, 1990) and some lines of potato (Grunn, personal communication). In contrast,…”

The cytochrome oxidase subunit I and subunit III genes in Oenothera mitochondria are transcribed from identical promoter sequences

“…Isolation of mtDNA and plasmid DNAs Linear and closed circular mtDNAs were isolated as described earlier (Brennicke, 1980;Brennicke and Blanz, 1982). Hybridization and DNA sequencing Heterologous hybridization conditions with maize genes into Oenothera mitochondrial DNA have been described by Hiesel and Brennicke (1983).…”