Mitochondrial DNA copy number measured by mitoscore is associated to trophectoderm quality

Santos, María José de los; Mercader, Amparo; Delgado, A.; Escrich, L.; Buendía, Pilar; Rubio, Carmen; Dı́ez-Juan, Antonio

doi:10.1016/j.rbmo.2017.10.031

Cited by 3 publications

(4 citation statements)

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“…Embryos with lower implantation potential and lower trophectoderm quality have a higher MitoScore, probably because of increased mitochondrial biogenesis. According to the association between trophectoderm quality and MitoScore, it seems that changes in mitochondrial biogenesis have a negative effect on the proliferative capacity of trophoblast and subsequent implantation [ 27 ]. Although stress results in elevating the metabolic output of the embryo as a compensatory mechanism, there is no document of association between perceived stress and increased MitoScore [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Association Between MitoScore, BMI, and Body Fat Percentage as a Predictive Marker for the Outcome of In-Vitro Fertilization (IVF)

Arora¹,

Collazo²,

Eisermann³

et al. 2022

Cureus

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BackgroundInfertility is defined as the inability to establish a pregnancy within 12 months of regular and unprotected sexual intercourse. In response to these problems, assisted reproductive techniques (ARTs) have made profound impacts on the therapeutic management of infertility. However, in-vitro fertilization (IVF) success rates are confounded by several internal and external factors. A relatively new approach to embryo assessment is known as MitoScore (Igenomix, Miami, USA). As a result, we sough to evaluate whether MitoScore can help in predicting in IVF outcomes, and to assess the relationship between MitoScore, BMI, and body fat percentage in determining the success of ARTs. MethodsUsing retrospective cohort, a study population consisting of 166 women aged 26-43 who were undergoing ART with pre-implantation genetic testing for aneuploidy (PGT-A) was assessed to determine if MitoScore, BMI, and body fat percentage impacted IVF outcomes. ResultsMitoScore, BMI, and body fat percentage were significantly lower in pregnant women as compared to nonpregnant women. Furthermore, MitoScore was correlated with subclasses of IVF outcomes (delivery, biochemical pregnancy, and spontaneous abortion) and was found to be positively correlated with BMI in patients with biochemical pregnancies. ConclusionOur findings suggest that MitoScore, BMI, and body fat percentage could act as critical parameters in determining the success of ART. However, the association between MitoScore, BMI, and body fat percentage does not appear to be a significant confounding factor to determine pregnancy outcome at this stage. Still, many factors need to be considered to establish the correlation reliably.

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Section: Discussionmentioning

confidence: 99%

Association Between MitoScore, BMI, and Body Fat Percentage as a Predictive Marker for the Outcome of In-Vitro Fertilization (IVF)

Arora¹,

Collazo²,

Eisermann³

et al. 2022

Cureus

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“…Three more single-clinic studies with large sample sizes ( n > 10 0) we re p Czech Republic), one from Bahceci IVF Center (Istanbul, Turkey), and one from IVI Valencia (Valencia, Spain). All three failed to establish any predictive power of implantation by mtDNA copy number [11][12][13]. Importantly, both latter studies relied on the services of Igenomix to quantify mtDNA in their embryos, thereby controlling for possible technical differences regarding mtDNA quantitation.…”

Section: Making Sense Of It Allmentioning

confidence: 99%

“…Importantly, both latter studies relied on the services of Igenomix to quantify mtDNA in their embryos, thereby controlling for possible technical differences regarding mtDNA quantitation. It is conceivable that all mentioned independent studies [7,8,[11][12][13]] randomly happened to be examples of those clinics in which mtDNA levels hold no predictive power at all. If this scenario is actually the case, future autonomous studies conducted blindly by additional clinics should reveal a clearer picture-so far, we are unaware of any clinic that having analyzed significant sample sizes, independently validated blastocyst mtDNA copy number as a biomarker of implantation.…”

Section: Making Sense Of It Allmentioning

confidence: 99%

Is mitochondrial DNA quantitation in blastocyst trophectoderm cells predictive of developmental competence and outcome in clinical IVF?

Viotti

Victor

Zouves

et al. 2017

J Assist Reprod Genet

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Behind every successful IVF embryo transfer, there is a great game of chance. Methods seeking to tilt the balance and increase the likelihood of implantation have been proposed and implemented with varying results, including embryo morphology, FISH-PGS, comprehensive chromosomal screening (CCS), morphokinetics, endometrial receptivity testing. It has been suggested that mitochondrial DNA (mtDNA) copy number could serve as a biomarker for embryo viability, but this concept was recently challenged. The world of IVF is left with unanswered questions: Why are there discrepancies in the reports? Should mtDNA copy number be considered to rank embryos for transfer? And in a broader sense, how well must a technique be validated before its implementation in the IVF clinic? Here, we explore these questions attempting to piece together the published data and suggest future directions to help unravel the subject matter.

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“…Victor et al reported a modified method that demonstrated mtDNA lacked of predictive value (17). Three independent studies presented that mtDNA content failed to establish predictive power of implantation at the conference of Preimplantation Genetic Diagnosis International Society (PGDIS) (19–21). Moreover, Treff et al demonstrated that levels of mtNDA didn’t predict the reproductive potential in the similar gold standard : double embryo transfer (DET)(18).…”

Section: Introductionmentioning

confidence: 99%

The mitochondrial DNA content can not predict the embryo viability

Dayang

et al. 2018

Preprint

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Objective: To investigate whether the mitochondrial DNA content could predict the embryo viability Design: Retrospective analysis. Setting: Reproductive genetics laboratory Patient(s): A total of 421 biopsied samples obtained from 129 patients Intervention(s): Embryo biopsies samples underwent whole genome amplification (WGA) and were tested by next generation sequencing (NGS) and array Comparative Genomic Hybridization (aCGH), 30 samples were selected randomly to undergo quantitative real-time polymerase chain reaction (qPCR). Main Outcome Measure(s): Those embryos which obtained the consistent chromosome status determined both aCGH and NGS platform were further classified.We investigated the relationship of mtDNA content with several factors including female patient age, embryo morphology, chromosome status, and live birth rate of both blastocysts and blastomeres. Result(s):A total of 386 (110 blastomeres and 276 blastocysts) out of 399 embryos showed consistent chromosome status outcome. We found no statistically difference was observed in aneuploid and euploid blastocysts (p=0.14), the same phenomenon was observed in aneuploid and euploid blastomeres (p=0.89). Similarly, the mtDNA content was independent of female patient age, embryo morphology and live birth rate. Conclusion(s):The mtDNA content did not provide a reliable prediction of the viability of blastocysts to initiate a pregnancy.

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Mitochondrial DNA copy number measured by mitoscore is associated to trophectoderm quality

Cited by 3 publications

References 0 publications

Association Between MitoScore, BMI, and Body Fat Percentage as a Predictive Marker for the Outcome of In-Vitro Fertilization (IVF)

Association Between MitoScore, BMI, and Body Fat Percentage as a Predictive Marker for the Outcome of In-Vitro Fertilization (IVF)

Is mitochondrial DNA quantitation in blastocyst trophectoderm cells predictive of developmental competence and outcome in clinical IVF?

The mitochondrial DNA content can not predict the embryo viability

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