Is mitochondrial DNA quantitation in blastocyst trophectoderm cells predictive of developmental competence and outcome in clinical IVF?

Viotti, Manuel; Victor, Andrea; Zouves, Christo; Barnes, Frank L.

doi:10.1007/s10815-017-1072-6

Cited by 22 publications

(19 citation statements)

References 19 publications

(39 reference statements)

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“…Secondly, Reprogenetics group’s data showed mtDNA content lacked of predict power in 17 out of 35 clinics that demonstrated anything linking mtDNA content and implantation was not a universal biological phenomenon (16). That suggested it was not scientific for analyzing the mtDNA content in embryo which came from many IVF clinics together.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the mtDNA content was further indicated to be negatively related to embryo viability and proposed to be used as a biomarker in the prediction of implanation outcome (4, 12, 14, 15). But these results were still under debate (16–18). Victor et al reported a modified method that demonstrated mtDNA lacked of predictive value (17).…”

Section: Introductionmentioning

confidence: 99%

“…It is worth noting that many multi-copy sequences (such as Alu) which comprise self-transposable elements, that may cause variability among the human [28, 29]. Secondly, Reprogenetics group’s data showed mtDNA content lacked of predict power in 17 out of 35 clinics that demonstrated anything linking mtDNA content and implantation was not a universal biological phenomenon (16). not scientific for analyzing the mtDNA content in embryo which came from many IVF clinics together.…”

Section: Introductionmentioning

confidence: 99%

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The mitochondrial DNA content can not predict the embryo viability

Dayang

et al. 2018

Preprint

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Objective: To investigate whether the mitochondrial DNA content could predict the embryo viability Design: Retrospective analysis. Setting: Reproductive genetics laboratory Patient(s): A total of 421 biopsied samples obtained from 129 patients Intervention(s): Embryo biopsies samples underwent whole genome amplification (WGA) and were tested by next generation sequencing (NGS) and array Comparative Genomic Hybridization (aCGH), 30 samples were selected randomly to undergo quantitative real-time polymerase chain reaction (qPCR). Main Outcome Measure(s): Those embryos which obtained the consistent chromosome status determined both aCGH and NGS platform were further classified.We investigated the relationship of mtDNA content with several factors including female patient age, embryo morphology, chromosome status, and live birth rate of both blastocysts and blastomeres. Result(s):A total of 386 (110 blastomeres and 276 blastocysts) out of 399 embryos showed consistent chromosome status outcome. We found no statistically difference was observed in aneuploid and euploid blastocysts (p=0.14), the same phenomenon was observed in aneuploid and euploid blastomeres (p=0.89). Similarly, the mtDNA content was independent of female patient age, embryo morphology and live birth rate. Conclusion(s):The mtDNA content did not provide a reliable prediction of the viability of blastocysts to initiate a pregnancy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The mitochondrial DNA content can not predict the embryo viability

Dayang

et al. 2018

Preprint

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“…In reproductive medicine identifying the embryo(s) with the highest implantation potential is still an unmet challenge, and it is a fundamental step toward single embryo transfer. During the years, several approaches have been proposed for embryo viability evaluation including embryo morphology, study of metabolic activity, extended culture, and both invasive and non-invasive preimplantation genetic testing [1,2,3,4]. Various embryological parameters have some predictive value for implantation potential.…”

Section: Introductionmentioning

confidence: 99%

Pronuclear score improves prediction of implantation rate in ICSI cycles

Stigliani

Massarotti

Casciano

et al. 2020

Preprint

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Background: In assisted reproduction technology embryo competence is routinely evaluated on morphological criteria but their efficacy remains relatively low. Additional information could be obtained by evaluation of pronuclear (PN) morphology. Up to now controversial results have been reported about prognostic value of PN score. One of the main limitation of literature data is the use of different methods of PN classification. To this regard, in 2011 the ESHRE and Alpha Scientists in Reproductive Medicine defined three PN categories to standardize the zygote assessment. In this study we evaluated whether the consensus ESHRE-Alpha system for the pronuclear scoring could be an useful additional criterion to improve prediction of embryo implantation potential.Methods: This is a retrospective, longitudinal cohort study. We included 3004 zygotes from 539 women who underwent ICSI treatment at our Center between January 2014 and June 2019. The pronuclear were categorized as score 1: symmetrical, 2: non-symmetrical, 3: abnormal. A subset of 110 zygotes did not cleaved. On day 2-3 1163 embryos were transferred, 232 arrested, and 9 were cryopreserved. Among the 1490 embryos cultured up to day 5-7, 516 became blastocysts: 123 were transferred on day 5 and 393 were cryopreserved. Relationship between pronuclear score and cleavage rate, quality of embryos, blastocyst development, and implantation rate was evaluated by chi-square test. Multivariate regression analyses corrected the results for putative confounders (age of patient; infertility cause; cleavage-stage embryo morphology grade; day of transfer). Results: There was not significant difference in patients’ age, cleavage rate and embryo morphology among the three pronuclear score groups. No reduction of blastulation rates was found in score 2-3 (34%) groups respect to score 1 (35%). The pronuclear score 1-embryos had a higher implantation rate respect to score 2-3-ones (15% and 9%, respectively, P=0.0121; OR 0.46; 95% CI 0.25-0.76, P=0.004). Consistently, the pronuclear score remained predictive of implantation in top quality embryos (OR 0.51; 95% CI 0.28-0.87, P= 0.01).Conclusions: The consensus pronuclear score may be routinely included among criteria for embryo evaluation to increase patient’s chance of becoming pregnant.

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“…Establishing a comprehensive roadmap of all cellular bioenergetics during developmental stages would offer a fundamental metabolic guide in the energetic requirements of somatic and germ-line cells, which is important for reproduction and healthy development [100][101][102], but also for tissue regeneration [95].…”

Section: The Use Of Animal Models To Study the Mtdna Bottleneckmentioning

confidence: 99%

Resolving the role of genetic defects and mtDNA copy number in mitochondrial disease and development

Kamps

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Is mitochondrial DNA quantitation in blastocyst trophectoderm cells predictive of developmental competence and outcome in clinical IVF?

Cited by 22 publications

References 19 publications

The mitochondrial DNA content can not predict the embryo viability

The mitochondrial DNA content can not predict the embryo viability

Pronuclear score improves prediction of implantation rate in ICSI cycles

Resolving the role of genetic defects and mtDNA copy number in mitochondrial disease and development

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