Mitochondrial D-loop nucleotide diversity in Astyanax (Osteichthyes, Characidae) from the upper Paraná and upper Paraguay River basins

Prioli, Alberto José; Carlo, V.A.; Soria, Thatiana Vanessa; Prioli, Sônia Maria Alves Pinto; Pavanelli, Carla Simone; Prioli, R.A.; Gomes, Vivian Nunes; Júlio, Horácio Ferreira; Prioli, L. M.

doi:10.4238/2012.april.27.5

Cited by 8 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Resultsmentioning

confidence: 82%

“…Sequences revealed the two haplogroups already reported by Prioli et al (2012), with an insertion or deletion events of 35 bp. Although Prioli et al (2012) reported a 32-bp indel, the difference seems to have been caused by a variation in sequence alignment. The specimen AstyPR-04 was reported with a 35-bp segment but with a nucleotide polymorphism compatible to that of haplogroup AltoPR-D, which does not feature the 35-bp segment.…”

Section: Resultsmentioning

confidence: 82%

“…In the wake of the recent development of species molecular diagnosis with investigations of mitochondrial sequences of the D-loop control region of Astyanax altiparanae, Soria (2004), Pie et al (2009) and Prioli et al (2012) discovered two haplogroups, which diverged at several sites, each having a 32-bp segment. According to Prioli et al (2012), molecular differentiation may be compared to species of other genera.…”

Section: Introductionmentioning

confidence: 99%

“…According to Prioli et al (2012), molecular differentiation may be compared to species of other genera. In addition, 5.1% of the nucleotides are diagnosed as haplogroup AltoPR and AltoPR-D (D is for deletion or lack of insertion) in the stretch of the D-loop region whose sequence was obtained.…”

Section: Introductionmentioning

confidence: 99%

“…If the hypothesis is correct, the species may be either a new species or an already described one, due to the morphological overlap in the bimaculatus group. Moreover, Soria (2004) and Prioli et al (2012) provided D-loop sequences of Astyanax asuncionensis Géry 1972, a species from the basin of the Paraguay and lower Paraná Rivers. A asuncionensis also belongs to the bimaculatus group, but on the basis of an uncertain diagnosis when compared with A. altiparanae.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Molecular study of Astyanax altiparanae (Osteichthyes, Characidae) as a probable species complex

et al. 2014

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Astyanax altiparanae, belonging to the bimaculatus group, which includes species with similar colors and morphology, occurs in the upper Paraná River basin. As the use of mitochondrial DNA has made great strides in the diagnosis of species, in previous researches, two strains were detected in A. altiparanae with a high divergence in the D-loop region, provisionally called AltoPR and AltoPR-D. Evidence led to the hypothesis that the two strains did not belong to the same species. Phylogenetic hypotheses were produced by maximum-likelihood. Mean internal distances of the AltoPR and AltoPR-D groups were respectively 0.002 and 0.003, with the distance between them being 0.037. Sequences from GenBank of specimens collected from the Paraíba do Sul River basin were also divided into two groups, of which one may be identified as AltoPR. Since the other group provided an intermediate distance when compared to AltoPR-D, an in-depth investigation was required. The other species analyzed showed a greater distance and was revealed to be a monophyletic taxon. The results suggested that they are really two species and that neither corresponds to the other species used in the current study.

show abstract

Section: Resultsmentioning

confidence: 82%

Section: Resultsmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular study of Astyanax altiparanae (Osteichthyes, Characidae) as a probable species complex

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

Chromosomal characterization in two species of an Astyanax bimaculatus complex (Characidae, Characiformes) using different techniques of chromosome banding

et al. 2015

View full text Add to dashboard Cite

Astyanax has been the subject of extensive cytogenetic studies due to its wide karyotypic diversity. This genus comprises species complexes, namely groups of fish of difficult morphological differentiation, such as the bimaculatus complex, which includes the characids with a rounded humeral spot. Thence, the present study proposed to accomplish a cytogenetic characterization of two species of this complex: A. asuncionensis and A. altiparanae, aiming to find chromosomal markers that differentiate these species, as well as achieve a better understanding of the karyotype evolution in the genus. For this we used different techniques of chromosome banding as C-banding, impregnation by silver nitrate, fluorochrome staining and FISH with 18S rDNA probe. This is the first cytogenetic study in A. asuncionensis, from Miranda river, which presented 2n = 50 and 18 m + 22sm + 6st + 4a (FN = 96) and single NORs. The populations of A. altiparanae also presented 2n = 50, but with different karyotypic formulae: the population of the Quexada river presented 16 m + 24sm + 4st + 6a (FN = 94) and the Esperança stream and Jacutinga river showed 16 m + 20sm + 4st + 10a (FN = 90). All analyzed populations showed an interindividual variation in the number and location of the nucleolar organizer regions (NORs). Single and multiple NORs were detected either by impregnation with silver nitrate or by FISH with 18S rDNA probe. After C-banding, the two species differed in relation to the composition and heterochromatin distribution. The meiotic cells of A. altiparanae male individuals were also analyzed, showing that, despite the high karyotype variability, chromosome pairing occurs normally. The data show that A. altiparanae and A. asuncionensis share some characteristics with other species of the bimaculatus complex, suggesting a close phylogenetic relationship among those species. However, some features can be used as differentiation chromosomal markers in altiparanae/asuncionensis morphotypes, which could result from a natural speciation process.

show abstract

Photoacoustic for thermal diffusivity determination of fish scale: A methodology for environmental integrity monitoring

Sabino

Súarez

Andrade

et al. 2022

Journal of Photochemistry and Photobiology B: Biology

View full text Add to dashboard Cite

Mitochondrial D-loop nucleotide diversity in Astyanax (Osteichthyes, Characidae) from the upper Paraná and upper Paraguay River basins

Cited by 8 publications

References 21 publications

Molecular study of Astyanax altiparanae (Osteichthyes, Characidae) as a probable species complex

Molecular study of Astyanax altiparanae (Osteichthyes, Characidae) as a probable species complex

Chromosomal characterization in two species of an Astyanax bimaculatus complex (Characidae, Characiformes) using different techniques of chromosome banding

Photoacoustic for thermal diffusivity determination of fish scale: A methodology for environmental integrity monitoring

Contact Info

Product

Resources

About