Mitochondrial aconitase controls adipogenesis through mediation of cellular ATP production

Chen, Yan; Cai, Guo He; Xia, Bo; Wang, Xin; Zhang, Cong Cong; Xie, Baocai; Shi, Xiaochen; Liu, Huan; Lu, Jun; Zhang, Rui Xin; Zhu, Meng Qing; Liu, Min; Yang, Shi Zhen; Zhang, Dan Yang; Chu, Xin; Khan, Rajwali; Wang, Yong Liang; Wu, Jianli

doi:10.1096/fj.201903224rr

Cited by 18 publications

(8 citation statements)

References 56 publications

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“…Aconitase 1 (Aco1) and aconitase 2 (Aco2), two aconitase isoforms, present in the cytoplasm and mitochondria, respectively [ 18 ]. Deficiency of Aco1 and Aco2 are associated with increased risk of optic atrophy and encephalopathy, respectively [ 21 , 22 ]. These diseases are caused by autosomal recessive mutations in Aco 1 and 2.…”

Section: Tca-related Enzymes and Diseases Arising From Their Dysfunctionmentioning

confidence: 99%

Emerging Role of TCA Cycle-Related Enzymes in Human Diseases

Kang

Suzuki

Saitô

et al. 2021

IJMS

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The tricarboxylic acid (TCA) cycle is the main source of cellular energy and participates in many metabolic pathways in cells. Recent reports indicate that dysfunction of TCA cycle-related enzymes causes human diseases, such as neurometabolic disorders and tumors, have attracted increasing interest in their unexplained roles. The diseases which develop as a consequence of loss or dysfunction of TCA cycle-related enzymes are distinct, suggesting that each enzyme has a unique function. This review aims to provide a comprehensive overview of the relationship between each TCA cycle-related enzyme and human diseases. We also discuss their functions in the context of both mitochondrial and extra-mitochondrial (or cytoplasmic) enzymes.

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Section: Tca-related Enzymes and Diseases Arising From Their Dysfunctionmentioning

confidence: 99%

Emerging Role of TCA Cycle-Related Enzymes in Human Diseases

Kang

Suzuki

Saitô

et al. 2021

IJMS

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“…In addition, other differential proteins have been reported to be associated with metabolism or obesity. Aconitate hydratase is involved in tricarboxylic acid cycle and carbohydrate metabolism, catalyzes the isomerization of citrate into isocitrate through cisaconite acid, and can control adipogenesis by mediating the production of cellular ATP [29]. Inhibin and activin can inhibit and activate pituitary secretion of follicle-stimulating hormone, respectively.…”

Section: Resultsmentioning

confidence: 99%

Comparison of urine proteome between obese people and normal weight people

Wang,

Wei,

Zhou

et al. 2024

Preprint

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Objective: To explore whether urine proteome can reflect the difference between obese and normal weight people. Methods: Urine samples from obese and normal weight people were collected and identified by non-label quantitative proteomics using high performance liquid chromatography tandem mass spectrometry (LC-MS/MS). The difference proteins of urine proteome between obese and normal weight people were screened for protein function and biological pathway analysis. The urine proteome of obese individuals was compared with that of normal weight people, and the common differential proteins were counted to analyze the protein function and biological pathways. Reported biomarkers of obesity were searched in the urine proteome of obese individuals. Results: 38 different proteins can be identified in the urine proteome of obese people compared with normal weight people, some of which have been reported to be related to metabolism and obesity, and the biological processes of differential proteins are also related to metabolism and other processes. 8 common differential proteins in the urine proteome of obese individuals and normal weight people, among which some proteins have been reported to be related to metabolism and obesity, and the biological processes of differential proteins are also related to metabolism and other processes. Among the differential proteins in the urine proteome of obese individuals compared with the normal weight people, the reported obesity biomarkers can be matched. Conclusions: The urine proteome can distinguish the obese people from the normal weight people, and the differential proteins in the urine proteome have key proteins that are known to be related to obesity and metabolism, and the biological processes of differential proteins also related biological processes such as nutrition and metabolism. Urine proteome has the potential to explore the pathogenesis of obesity and provide personalized treatment.

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“…Both extra- and intra-cellular ATP levels are shown to modulate adipogenesis [15, 46]. Although mitochondria becomes metabolically active during adipogenesis however a reduction in cellular ATP is reported [47].…”

Section: Discussionmentioning

confidence: 99%

TRPV4 modulation affects mitochondrial parameters in adipocytes and its inhibition upregulates lipid accumulation

Kumar,

Acharya,

Kumar

et al. 2024

Preprint

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Enhanced lipid-droplet formation by adipocytes is a complex process and relevant for obesity. Using knock-out animals, involvement of TRPV4, a thermosensitive ion channel in the obesity has been proposed. However, exact role/s of TRPV4 in adipogenesis and obesity remain unclear and contradictory. Here we used in vitro culture of 3T3L-1 preadipocytes and primary murine-mesenchymal stem cells as model systems, and a series of live-cell-imaging to analyse the direct involvement of TRPV4 exclusively at the adipocytes that are free from other complex signalling as expected in in-vivo condition. Functional TRPV4 is endogenously expressed in pre- and in mature-adipocytes. Pharmacological inhibition of TRPV4 enhances differentiation of preadipocytes to mature adipocytes, increases expression of adipogenic and lipogenic genes, enhances cholesterol, promotes bigger lipid-droplet formation and reduces the lipid droplet temperature. TRPV4 regulates mitochondrial-temperature, Ca2+-load, ATP, superoxides, cardiolipin, membrane potential (ΔΨm), and lipid-mitochondrial contact sites. TRPV4 also regulates the extent of actin fibres, affecting the cells mechanosensing ability. These findings link TRPV4-mediated mitochondrial changes in the context of lipid-droplet formation involved in adipogenesis and confirms the direct involvement of TRPV4 in adipogenesis. These findings may have broad implication in treating adipogenesis and obesity in future.

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Mitochondrial aconitase controls adipogenesis through mediation of cellular ATP production

Cited by 18 publications

References 56 publications

Emerging Role of TCA Cycle-Related Enzymes in Human Diseases

Emerging Role of TCA Cycle-Related Enzymes in Human Diseases

Comparison of urine proteome between obese people and normal weight people

TRPV4 modulation affects mitochondrial parameters in adipocytes and its inhibition upregulates lipid accumulation

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