Mitochondria-targeting fluorescent molecules for high efficiency cancer growth inhibition and imaging

Chen, Hao; Wang, Jing; Feng, Xin; Zhu, Mark; Hoffmann, Simon; Hsu, Alexander; Qian, Kun; Huang, Daijuan; Zhao, Feng; Liu, Wei; Zhang, Huimao; Cheng, Zhen

doi:10.1039/c9sc01410a

Cited by 49 publications

(38 citation statements)

References 34 publications

(36 reference statements)

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“…Of particular interest is the development of fluorescent probes to improve the performance of super-resolution imaging in probing mitochondria-lysosome interactions 10,11 . Numerous synthetic fluorescent probes have been developed to prolong photobleaching resistance 12 , reduce phototoxicity 13 , decrease background signal 11 , and inhibit tumor-growth 14 for imaging the morphology of subcellular organelles. However, at least two fluorescent probes with different emission spectra-small-molecule dyes 3 or fluorescent proteins 2 -are needed to monitor mitochondria-lysosome interplay.…”

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confidence: 99%

A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

et al. 2020

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Mitochondria–lysosome interactions are essential for maintaining intracellular homeostasis. Although various fluorescent probes have been developed to visualize such interactions, they remain unable to label mitochondria and lysosomes simultaneously and dynamically track their interaction. Here, we introduce a cell-permeable, biocompatible, viscosity-responsive, small organic molecular probe, Coupa, to monitor the interaction of mitochondria and lysosomes in living cells. Through a functional fluorescence conversion, Coupa can simultaneously label mitochondria with blue fluorescence and lysosomes with red fluorescence, and the correlation between the red–blue fluorescence intensity indicates the progress of mitochondria–lysosome interplay during mitophagy. Moreover, because its fluorescence is sensitive to viscosity, Coupa allowed us to precisely localize sites of mitochondria–lysosome contact and reveal increases in local viscosity on mitochondria associated with mitochondria–lysosome contact. Thus, our probe represents an attractive tool for the localization and dynamic tracking of functional mitochondria–lysosome interactions in living cells.

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confidence: 99%

A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

et al. 2020

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“…S41c and d †), electrostatic interaction and long-alkyl chain-induced hydrophobicity would enable it to rmly stain mitochondria, which was also reported in the previous literature. 60 The turn-on uorescence when EBD-5 anchored on mitochondria could be attributed to the interaction between EBD-5 and mitochondrial DNA [61][62][63][64][65][66] (Fig. S41e †).…”

Section: Cell Membrane Targeting and Specic Targeting Mechanismsmentioning

confidence: 99%

A NIR-I light-responsive superoxide radical generator with cancer cell membrane targeting ability for enhanced imaging-guided photodynamic therapy

Zhu

et al. 2020

Chem. Sci.

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“…Cancer is a major disease that threatens human life. Recently, nanomedicine has enabled early diagnosis and precise treatment of various tumors, thereby bringing new hope for cancer management [1][2][3][4]. Generally, tumors possess an inherent pathologically leaky vasculature and defective lymphatics.…”

Section: Introductionmentioning

confidence: 99%

Soft Mesoporous Organosilica Nanoplatforms Improve Blood Circulation, Tumor Accumulation/Penetration, and Photodynamic Efficacy

et al. 2020

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To date, the ability of nanoplatforms to achieve excellent therapeutic responses is hindered by short blood circulation and limited tumor accumulation/penetration. Herein, a soft mesoporous organosilica nanoplatform modified with hyaluronic acid and cyanine 5.5 are prepared, denoted SMONs-HA-Cy5.5, and comparative studies between SMONs-HA-Cy5.5 (24.2 MPa) and stiff counterparts (79.2 MPa) are conducted. Results indicate that, apart from exhibiting a twofold increase in tumor cellular uptake, the soft nanoplatforms also display a remarkable pharmacokinetic advantage, resulting in considerably improved tumor accumulation. Moreover, SMONs-HA-Cy5.5 exhibits a significantly higher tumor penetration, achieving 30-μm deeper tissue permeability in multicellular spheroids relative to the stiff counterparts. Results further reveal that the soft nanoplatforms have an easier extravasation from the tumor vessels, diffuse farther in the dense extracellular matrix, and reach deeper tumor tissues compared to the stiff ones. Specifically, the soft nanoplatforms generate a 16-fold improvement (43 vs. 2.72 μm) in diffusion distance in tumor parenchyma. Based on the significantly improved blood circulation and tumor accumulation/penetration, a soft therapeutic nanoplatform is constructed by loading photosensitizer chlorin e6 in SMONs-HA-Cy5.5. The resulting nanoplatform exhibits considerably higher therapeutic efficacy on tumors compared to the stiff ones.

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Mitochondria-targeting fluorescent molecules for high efficiency cancer growth inhibition and imaging

Abstract: 5BMF is a new fluorescent mitochondria-accumulating delocalized lipophilic cations [DLC] that boasts significantly increased anti-cancer effects and low toxicity in comparison to previous DLCs, addressing current hurdles in DLC clinical translation.

Cited by 49 publications

References 34 publications

A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

A NIR-I light-responsive superoxide radical generator with cancer cell membrane targeting ability for enhanced imaging-guided photodynamic therapy

Soft Mesoporous Organosilica Nanoplatforms Improve Blood Circulation, Tumor Accumulation/Penetration, and Photodynamic Efficacy

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