Mitochondria Research in Human Reproduction

Lee, Yi-Xuan; Lin, Pei‐Hsuan; Rahmawati, Endah; Ma, Yun-Yi; Chan, Cindy; Tzeng, Chii‐Ruey

doi:10.1016/b978-0-12-813209-8.00020-0

Cited by 12 publications

(9 citation statements)

References 66 publications

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“…Mitochondrial membrane potential is an important measure of the absence of mitochondrial activity. The loss of mitochondrial membrane potential may cause the release of apoptotic factors that cause cell death . Compounds 4f, 4h, 4i, 4k , 4l , and cisplatin were tested to identify the mitochondrial membrane potential.…”

Section: Resultsmentioning

confidence: 99%

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Synthesis of Some New 1,3,4-Oxadiazole Derivatives and Evaluation of Their Anticancer Activity

Yurttaş,

Evren,

Kubilay

et al. 2023

ACS Omega

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In this work, some new 2-[(5-((2-acetamidophenoxy)methyl)-1,3,4-oxadiazol-2-yl)thio]acetamide derivatives ( 4a-4l ) were synthesized and studied for their anticancer activity. Twelve new compounds were tested on the A549 human lung cancer cell line, C6 rat glioma cell line, and L929 murine fibroblast cell line. Compounds 4f, 4i, 4k , and 4l (IC 50 : 1.59–7.48 μM), and especially 4h (IC 50 : <0.14 μM), exhibited excellent cytotoxic profile on A549 with selectivity. Compounds 4g and 4h showed remarkable antiproliferative activity on the C6 cell line with IC 50 values of 8.16 and 13.04 μM, respectively. The compounds with the lowest IC 50 value on the A549 cell line ( 4f, 4h, 4i, 4k , and 4l ) were further studied to determine the mechanism of action. These compounds were found to induce apoptosis with a higher ratio (16.10–21.54%) than that of the standard drug cisplatin (10.07%). Compound 4f displayed mitochondrial membrane depolarization and caspase-3 activation at most, whereas compounds 4h (89.66%) and 4i (78.78%) had outstanding retention rates in the G0/G1phase of the cell cycle (cisplatin 74.75%). Compounds 4f, 4g, 4h , and 4l exhibited matrix metalloproteinase-9 (MMP-9) inhibition higher than 75% at 100 μg/mL; even IC 50 values were found to be 1.65 and 2.55 μM for 4h and 4l . In addition, in silico physicochemical properties of the compounds and molecular docking interaction of compound 4h on the MMP-9 enzyme were evaluated; the desired and expected results were obtained.

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Section: Resultsmentioning

confidence: 99%

“…The loss of mitochondrial membrane potential may cause the release of apoptotic factors that cause cell death. 43 Compounds 4f, 4h, 4i, 4k, 4l, and cisplatin were tested to identify the mitochondrial membrane potential. The test results are listed in Table 4 and Figure control cells.…”

Section: Mitochondrial Membrane Depolarizationmentioning

confidence: 99%

Synthesis of Some New 1,3,4-Oxadiazole Derivatives and Evaluation of Their Anticancer Activity

Yurttaş,

Evren,

Kubilay

et al. 2023

ACS Omega

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“…Mitochondrial membrane potential (Δψm) is the "driving force" of ATP synthesis by mitochondria so it is the main indicator of the level of mitochondrial activity, reflects the process of electron transport and oxidative phosphorylation, and is closely related to the capacity of mitochondria to produce ATP. Depolarization below the Δψm threshold indicates mitochondrial dysfunction and is a prerequisite for mitophagy mechanisms that play a role in maintaining mitochondrial homeostasis [12]- [14].…”

Section: Discussionmentioning

confidence: 99%

Effect of Exposure to Follicular Fluid in Endometrioma Patients on the Presence of Polar Body I, Distribution Pattern and Intensity of Mitochondria Oocyte Fluorescence

Chandragiram¹,

Jaya²,

Sudiman³

et al. 2022

EJMED

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Introduction: Mitochondria are small organelles that are directly involved in many essential cellular functions. Mitochondria are very sensitive to the surrounding environmental conditions and are easily affected by various free radicals or ROS. Endometriosis is a disease associated with increased ROS. The effect of endometriosis on oocyte mitochondrial abnormalities or dysfunction has received limited attention. This study aims to determine the effect of exposure to follicular fluid in endometrioma patients on the presence of polar body I, distribution pattern, and intensity of mitochondrial fluorescence in mice oocytes. Methods: The study design was a randomized post-test only control group design using oocytes of immature Swiss mice exposed to follicular fluid from endometrioma patients. Follicular fluid was taken at the time of picking oocytes from infertility patients who participated in the FIV-ISIS program. Immature oocytes were matured in vitro (IVM) in culture media with follicular fluid added from endometrioma and non-endometriotic patients as a control. The presence of polar body I (oocyte maturation), fluorescence intensity (amount/metabolic activity) and mitochondrial distribution pattern were compared in the two groups. Data analysis with SPSS 16.0 program. Variable analysis was done by chi square test and independent t test. Results: Polar body I was significantly lower (30% vs 75%) in the treatment group compared to the control group (p=0,01). The pattern of diffuse distribution (30% vs 70%) was significantly lower in the treatment group compared to the control group (p=0,027). The mean fluorescence intensity (556,54 268.96 vs 818,07228.17) was significantly lower in the treatment group compared to the control group (p<0,001). Conclusion: The effect of exposure to follicular fluid in endometrioma patients significantly reduced the presence of polar body I, caused a change in distribution pattern and decreased the intensity of mitochondrial fluorescence in mice oocytes.

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“…Therefore, PPS might have great potential to ameliorate atherosclerosis due to its capacity of minimizing endothelial cell apoptosis. Mitochondrial membrane potential is a key indicator of mitochondrial activity because it re ects the process of electron transport and oxidative phosphorylation, and the driving force behind ATP production [30]. The dissipation of MMP constitutes an early irreversible step in the cascade of events leading to apoptosis [31].…”

Section: Rt-qpcr Analysismentioning

confidence: 99%

Protective effect of phosphorylated Athyrium multidentatum (Doll.) Ching polysaccharide on vascular endothelial cells in vitro and in vivo

Yin

Chen

Miao

et al. 2023

Preprint

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The purpose of this study was to prepare phosphorylated Athyrium multidentatum (Doll.) Cing polysaccharide (PPS) and investigate its protective effect on vascular endothelial cells (VECs) in vitro and in vivo and the underlying mechanisms. Sodium tripolyphosphate (STPP) and sodium trimetaphosphate (STMP) were used as phosphorylation reagents and PPS was characterized by Fourier transform infrared (FT-IR), 13C nuclear magnetic resonance (13C NMR) and 31P nuclear magnetic resonance (31P NMR) spectra. Chemical analysis demonstrated that PPS was composed of mannose, glucosamine, rhamnose, glucuronic acid, galacturonic acid, galactosamine, glucose, galactose, xylose, arabinose and fucose with a molar ratio of 11.36:0.42:4.03:1.12:1.81:0.26:33.25:24.12:6.85:14.46:2.32 and a molecular weight of 28837 Da. Results from in vitro and in vivo assays revealed that PPS protected human umbilical vein endothelial cells (HUVECs) against H2O2-induced oxidative injury and attenuated VECs damage in mice treated with D-galactose. RNA sequencing (RNA-seq) analysis disclosed that PPS down- or up-regulated the expression of eighteen differentially expressed genes (DEGs) involved in the functions of vascular endothelium repairment, cell growth and proliferation, cell survival and apoptosis, inflammation, angiogenesis and antioxidant in mice abdominal aorta, implying that these biological processes might play crucial roles in the protective actions of PPS on VECs.

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Mitochondria Research in Human Reproduction

Cited by 12 publications

References 66 publications

Synthesis of Some New 1,3,4-Oxadiazole Derivatives and Evaluation of Their Anticancer Activity

Synthesis of Some New 1,3,4-Oxadiazole Derivatives and Evaluation of Their Anticancer Activity

Effect of Exposure to Follicular Fluid in Endometrioma Patients on the Presence of Polar Body I, Distribution Pattern and Intensity of Mitochondria Oocyte Fluorescence

Protective effect of phosphorylated Athyrium multidentatum (Doll.) Ching polysaccharide on vascular endothelial cells in vitro and in vivo

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