Mitochondria influence glycolytic and tricarboxylic acid cycle metabolism under postmortem simulating conditions

Matarneh, Sulaiman K.; Yen, Con‐Ning; Bodmer, Jocelyn; El‐Kadi, Samer W.; Gerrard, David E.

doi:10.1016/j.meatsci.2020.108316

Cited by 24 publications

(23 citation statements)

References 30 publications

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“…The earlier results revealed that mitochondria were related to energy metabolism in PM skeletal muscles. This result was consistent with the report of Matarneh et al ., 30 who found that mitochondrial can significantly contribute to energy metabolism. Moreover, England et al 31 .…”

Section: Discussionsupporting

confidence: 94%

Proteomic analyses of mitochondrial damage in postmortem beef muscles

Liu

Wei

et al. 2022

J Sci Food Agric

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BACKGROUND The objective of the study was to examine the expression profiles of mitochondrial proteins in at‐death and 24 h postmortem (PM) using tandem mass tag (TMT) approach to characterize the mitochondria possible mechanisms that are affiliated with tenderization. RESULTS Results showed that the tender meat at 24 h PM emerged with more serious mitochondrial damage. Altogether 456 mitochondrial proteins were identified, including 442 down‐regulated and 14 up‐regulated proteins. These differentially‐expressed proteins were primarily involved in the progress of PM energy metabolism, apoptosis, and the morphological alterations of mitochondrial. Among them, 47 subunits (such as NDUFA2, COX4I1, and ATP5PB) were annotated into the oxidative phosphorylation pathway. VDAC1, VDAC2, and VDAC3 involving in the damage of MPTP, and IMMT, CHCHD3, APOL and APOO modulating the morphology of mitochondria, and DIABLO and AIFM1 released from mitochondria affect caspase's activation. HSPD1 and HSPE1 involved in apoptosis, mitochondrial physiological and morphological alterations. CONCLUSION The earlier‐mentioned proteins were validated as potential indicators of tenderness regulated by mitochondrial damage. These results highlighted that mitochondrial damage possibly participate in PM tenderization of beef muscles by energy metabolism and cell apoptosis status. © 2022 Society of Chemical Industry.

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Section: Discussionsupporting

confidence: 94%

Proteomic analyses of mitochondrial damage in postmortem beef muscles

Liu

Wei

et al. 2022

J Sci Food Agric

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“…Wu et al (2020) considered new evidence of mitochondrial functionality under conditions that simulate those found in postmortem muscle based on mitochondrial appearance after being enriched with TCA cycle metabolites. Matarneh et al (2021) suggested that coupled respiration still existed in postmortem muscles before proceeding to prove residual mitochondrial functionality. Thus, in inducing PDK‐1 to adapt to the limited available oxygen, HIF‐1α could reduce the total amount of oxygen consumed per cell (Blum et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

“…It is worth noting that the mitochondrial membrane is also needed to import proteins during hypoxia while ensuring its maintenance (Martínez-Reyes et al, 2016). Thus, the results suggests that the ability of the mitochondria to transport substrates under hypoxic conditions is lower than that of normal organelles (England et al, 2018;Matarneh et al, 2021). Moreover, pyruvate, as the final product of glycolysis, serves as a substrate for mitochondrial respiration (Shen et al, 2014).…”

Section: Mitochondrial F0f1-atp Enzyme Activity In Postmortem Yak Musclementioning

confidence: 99%

Study on the HIF ‐1α regulated by glycolytic pathways and mitochondrial function in yaks of different altitudes during postmortem aging

Xin

Han

et al. 2022

Journal of Food Biochemistry

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The study investigated the glycolysis pathway mediated by hypoxia-inducible factor-1α (HIF-1α) and the mechanism of its regulation. The results indicated that HIF-1α expression initially increased before subsequently decreasing with aging time during postmortem (p < .01). Glucose transporter-1 (GLUT-1), lactate dehydrogenase (LDH), and hexokinase (HK) displayed a similar trend with aging time (p < .01) while pyruvate dehydrogenase kinase 1 (PDK-1) increased gradually within the first 12 hr before decreasing at 24-120 hr. However, after treatment with a HIF-1α inhibitor, no significant differences were observed in the mitochondrial morphology. Furthermore, lactate content decreased, along with LDH, HK, and F0F1-ATP activities as well as GLUT-1 and PDK-1 expression (p < .01). The shear force for all groups also increased during postmortem aging (p < .01), with that of the controls being significantly higher compared with the treatment groups (p < .01). These findings confirmed that, after slaughter, the hypoxic environment within the muscles provided essential conditions for HIF-1α expression, which, in turn, activated the glycolysis pathway by mediating changes in the activities of glycolytic enzymes and mitochondrial function. Moreover, in accelerating glycolysis rate, the expression of HIF-1α further played a negative role in meat tenderization during postmortem aging. This, it was concluded that HIF-1α expression plays a significant role in postmortem yak meat tenderization by regulating the glycolysis pathway.Pratical applications: While converting muscle into meat through hypoxic glycolysis during postmortem aging is undeniable, the biochemical mechanism of this process mediated remains quite obscure. However, the meat quality difference which impact muscle regulation mechanism during postmortem aging has not been reported. The study investigated the HIF-1α played a major role in both the glycolytic pathway and as well as meat tenderness during the postmortem aging of yak meat. The glycolysis pathway is mediated by hypoxia-inducible factor-1α (HIF-1α), the mechanism of its regulation, and meat tenderness during the postmortem aging of yak meat.

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“…Derivatization prior to GC-MS analyses oen includes laborious and time-consuming steps such as long incubation with the derivatization reagent and/or extraction of the derivatized carboxylates into an organic solvent. 9 In contrast with GC-MS, LC-MS offers separation of underivatized carboxylic acids. However, to reach sufficient separation and peak symmetry, ion-pairing reagents such as tributylamine were previously used in LC-MS, 10,11 which results in a long analysis time.…”

Section: Introductionmentioning

confidence: 99%