Mitochondria in teleost spermatozoa

Ulloa‐Rodríguez, Patricio; Figueroa, Elías; Díaz, Rommy; Lee‐Estévez, Manuel; Short, Stefanía; Farías, Jorge G.

doi:10.1016/j.mito.2017.01.001

Cited by 39 publications

(29 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, the sperm ATP content decreased in captive-reared greater amberjack from the EARLY phase to the SPAWNING phase. The ATP content is widely used as a sperm quality marker (Cosson et al, 2008b;Fauvel et al, 2010), because it is a key limiting factor for maintaining motility (Christen et al, 1987;Cosson, 2010;Ulloa-Rodríguez et al, 2017). Therefore, the decrease of energy content observed in captivereared greater amberjack in the present study might explain, at least partially, the lower percentage of motile spermatozoa.…”

Section: Discussionmentioning

confidence: 60%

Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810)1

Zupa

Fauvel

Mylonas

et al. 2017

Journal of Animal Science

View full text Add to dashboard Cite

The greater amberjack, (Risso, 1810), is a promising candidate for the diversification of European aquaculture production, but inconsistent reproduction in captivity prevents commercial production. Recent studies showed that greater amberjack confined in sea cages exhibited scarce gonad development and early interruption of gametogenic activity during the reproductive season. The aim of the present study was to improve our understanding of the observed impairment of spermatogenesis. Adult wild and captive-reared males were sampled during 3 different phases of the reproductive cycle: early gametogenesis (EARLY; late April to early May), advanced gametogenesis (ADVANCED; late May to early June), and spawning (SPAWNING; late June to July). Spermatogonial stem cells and proliferating germ cells were identified through the immunohistochemical localization of and proliferating cell nuclear antigen, respectively. Apoptotic germ cells were identified throughout the terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end labeling method. Sperm quality of captive-reared fish was evaluated using computer-assisted sperm analysis. Captive-reared males exhibited seminiferous lobules of a smaller diameter, a precocious and progressive decrease of spermatogonial mitosis, and a high level of apoptosis at the beginning of the reproductive season, concomitant with a many-fold higher 17β-estradiol plasma concentration. The motile spermatozoa percentage of captive greater amberjack was lower than in other teleosts, and a drastic decrease of spermatozoa motility duration, velocity, and ATP content occurred along the reproductive season. An abnormal increase of sperm concentration as well as an increase of dead spermatozoa occurred during the SPAWNING phase, probably because of lack of sperm hydration and ejaculation and consequent sperm ageing. The present study demonstrates the extreme susceptibility of greater amberjack to rearing stress and underscores the need for improvement of the rearing and handling procedures to ameliorate gametogenesis dysfunctions in commercial aquaculture production.

show abstract

Section: Discussionmentioning

confidence: 60%

Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810)1

Zupa

Fauvel

Mylonas

et al. 2017

Journal of Animal Science

View full text Add to dashboard Cite

show abstract

“…In many fish species, measurement of respiratory activity presents difficulties because of the low oxygen consumption of spermatozoa, in contrast to model species such as sea urchin [71]; in addition, the low respiratory activity remains almost unchanged when fish spermatozoa are transferred into motility-activating solutions [72], while it is about 50-fold increased when sea urchin sperm is transferred into sea water [71]. Efficient respiration needs to be coupled in mitochondria to ATP production via the ATP synthase [73].…”

Section: Mitochondrial Respirationmentioning

confidence: 99%

Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Cosson¹

2019

Biological Research in Aquatic Science

View full text Add to dashboard Cite

For reproduction, most fish species adopt external fertilization: their spermatozoa are delivered in the external milieu (marine-or freshwater) that represents both a drastic environment and a source of signals that control the motility function. This chapter is an updated overview of the signaling pathways going from external signals such as osmolarity and ionic concentration and their membrane reception to their transduction through the membrane and their final reception at the flagellar axoneme level. Additional factors such as energy management will be addressed as they constitute a limiting factor of the motility period of fish spermatozoa. Modern technologies used nowadays for quantitative description of fish sperm flagella in movement will be briefly described as they are more and more needed for prediction of the quality of sperm used for artificial propagation of many fish species used in aquaculture. The chapter will present some applications of these technologies and the information to which they allow access in some aquaculture species.Biological Research in Aquatic Science 2 the current knowledge with respect to (1) membrane reception of the activation signal and its transduction through the spermatozoon plasma membrane via the external membrane components such as ion channels or aquaporins; (2) cytoplasmic trafficking of the activation signal; (3) final steps of the signaling, including signal transduction to the axonemal machinery, and activation of axonemal dynein motors and regulation of their activity; (4) signaling involved in guidance processes that control the sperm/egg approach and meeting; and (5) pathways supplying energy for the short flagellar motility period of fish spermatozoa. For each step in this signaling process, quantitative methods were developed to evaluate the quality of the sperm samples that are used for aquaculture propagation of many fish species. These methods as well as examples of their usefulness for application to fish artificial reproduction are presented in the last part of this chapter. Fish sperm structure and spermatogenesisThe main traits of the flagellar mechanics of spermatozoa and the main factors that regulate their motility have been described in details in a recent review chapter by Cosson et al. [1]. Structure of fish spermatozoa: a brief presentationCompared to mammalian spermatozoa, the structure of a fish spermatozoon is qualified as "simple sperm", mostly because the flagellum structure does not include additional columns flanking the motor part (axoneme) that are present in mammal sperm. In teleost fish, spermatozoa generally have no acrosome (in contrast to chondrostean such as sturgeon), and the impenetrable chorion presents a micropyle that gives access to the membrane of the oocyte. Spermatozoa often show a spherical nucleus with homogenous, highly condensed chromatin, a nuclear fossa, a midpiece of variable size with or without a cytoplasmic channel, and one or two long flagella [2]. Moreover, fish spermatozoa can be classified into two forms, a...

show abstract

“…Mitochondria are essential for providing energy to flagellum and their number and function have been positively correlated with sperm motility and fertilization capacity [44]. Assessment of mitochondrial functionality by measuring mitochondrial membrane potential can be useful as a marker of fish sperm quality [45]. Some stressful procedures can affect sperm motility through two types of damage: direct damage to mitochondrial DNA or alterations to the inner or outer membrane [46].…”

Section: Discussionmentioning

confidence: 99%

Efficiency and cell viability implications using tip type electroporation in zebrafish sperm cells

et al. 2020

View full text Add to dashboard Cite

Sperm-mediated gene transfer (SMGT) has a potential use for zebrafish transgenesis. However, transfection into fish sperm cells still needs to be improved. The objective was to demonstrate the feasibility of tip type electroporation in zebrafish sperm, showing a protocol that provide high transfection efficiency, with minimal side-effects. Sperm was transfected with a Cy3-labelled DNA using tip type electroporation with voltages ranging from 500 to 1500 V. Sperm kinetics parameters were assessed using Computer Assisted Semen Analysis (CASA) and cell integrity, reactive oxygen species (ROS), mitochondrial functionality and transfection rate were evaluated by flow cytometry. The transfection rates were positively affected by tip type electroporation, reaching 64.9% ± 3.6 in the lowest voltage used (500 V) and 86.6% ± 1.9 in the highest (1500 V). The percentage of overall motile sperm in the electrotransfected samples was found to decrease with increasing field strength (P < 0.05). Increase in the sperm damaged plasma membrane was observed with increasing field strength (P < 0.05). ROS and sperm mitochondrial functionality did not present a negative response after the electroporation (P > 0.05). Overall results indicate that tip type electroporation enhances the internalization of exogenous DNA into zebrafish sperm cells with minimal harmful effects to sperm cells.

show abstract

Mitochondria in teleost spermatozoa

Cited by 39 publications

References 71 publications

Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810)1

Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810)1

Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Efficiency and cell viability implications using tip type electroporation in zebrafish sperm cells

Contact Info

Product

Resources

About