Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810)1

Zupa, Rosa; Fauvel, Christian; Mylonas, Constantinos C.; Pousis, Chrysovalentinos; Santamaría, N.; Papadaki, Maria; Fakriadis, Ioannis; Cicirelli, Vincenzo; Mangano, Salvatore; Passantino, Letizia; Lacalandra, Giovanni Michele; Corriero, Aldo

doi:10.2527/jas.2017.1708

Cited by 13 publications

(50 citation statements)

References 50 publications

(77 reference statements)

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“…One possibility is that the lower sperm quality observed was a result of sampling from captive-bred individuals (e.g., Locatello et al, 2018;Morato et al, 2001;Zupa et al, 2017). One possibility is that the lower sperm quality observed was a result of sampling from captive-bred individuals (e.g., Locatello et al, 2018;Morato et al, 2001;Zupa et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Applying cryopreservation to anuran conservation biology

Poo

Hinkson

2019

Conservat Sci and Prac

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Novel conservation methods have become increasingly important in protecting our remaining biodiversity in the face of unprecedented rates of species declines. One method of maintaining genetic and species diversity is through germplasm cryopreservation. However, our knowledge of cryopreservation relies heavily on studies in domestic or aquaculture species. Moreover, most studies are narrowly focused on a single species. Consequently, the broader application of these methods to wildlife conservation is often unknown or assumed. Here, we examine the response of four anuran species from two families (Anaxyrus fowleri, Anaxyrus baxteri, Lithobates pipiens, and Lithobates sevosus) to three sperm cryopreservation treatments (5, 10, and 15% N,N-dimethylformamide with 10% trehalose). Within each family, we selected a congeneric species pair with one common, nonthreatened species and one endangered species. We found that endangered species had significantly lower initial sperm quality, though recovery rate of sperm after cryopreservation was not significantly different between nonthreatened and endangered species. Overall, responses to the three treatments were consistent across species, with 5% DMFA with 10% trehalose producing the highest recovery rates in all four species. In addition, cryopreserved sperm were used to successfully produce hatchlings in both of the nonthreatened species. Our results demonstrate that standardized methods developed for anurans can be applied more widely across families and can be transferred from model species to species of conservation concern.These findings form a basis for further exploration into cryopreservation as an effective tool for wildlife conservation in amphibians. K E Y W O R D Samphibian, assisted reproductive technology, Bufonidae, cryobiology, Ranidae, sperm motility

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Section: Discussionmentioning

confidence: 99%

Applying cryopreservation to anuran conservation biology

Poo

Hinkson

2019

Conservat Sci and Prac

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“…For the present study, 25 (11 male and 14 female) liver samples taken from wild greater amberjack and 22 (10 male and 12 female) liver samples taken from captive‐reared greater amberjack were used. The samples were part of a larger sampling carried out during the reproductive season 2014 and 2015 in the framework of a study on the effects of rearing in captivity on gametogenesis and reproduction (Pousis et al, , ; Zupa, Fauvel, et al, ; Zupa, Rodríguez, et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…The reproductive state of the sampled fish was described by Zupa, Fauvel, et al () and Zupa, Rodríguez, et al (). Briefly, the fish (both wild and captive‐reared) sampled during late April–early June were in active gametogenesis, since males showed all stages of spermatogenesis and females had oocytes in early and/or late vitellogenesis.…”

Section: Methodsmentioning

confidence: 99%

“…Recent studies conducted in the Mediterranean Sea showed that the greater amberjack, as many other fishes, exhibits reproductive dysfunctions when caught from the wild and confined in captivity (Pousis et al, , ; Zupa, Fauvel, et al, ; Zupa, Rodríguez, et al, ). Captive‐reared greater amberjack females sampled during the reproductive season displayed an extensive atresia of advanced vitellogenic oocytes (Pousis et al, ; Zupa, Rodríguez, et al, ), which was possibly caused by stress during handling operations in the rearing cages (Pousis et al, , ; Zupa, Fauvel, et al, ; Zupa, Rodríguez, et al, ). Atretic degeneration of ovarian follicles and apoptosis of germ cells in males are normal physiological events of vertebrate gonadal morphogenesis that seem to be essential for the regulation of the energetic homoeostasis and for the selection of germ cells more suitable for the production of viable embryos (Corriero et al, ; Krysko et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…atresia of advanced vitellogenic oocytes (Pousis et al, 2018;Zupa, Rodríguez, et al, 2017b), which was possibly caused by stress during handling operations in the rearing cages (Pousis et al, 2018(Pousis et al, , 2019Zupa, Fauvel, et al, 2017a;Zupa, Rodríguez, et al, 2017b). Atretic degeneration of ovarian follicles and apoptosis of germ cells in males are normal physiological events of vertebrate gonadal morphogenesis that seem to be essential for the regulation of the energetic homoeostasis and for the selection of germ cells more suitable for the production of viable embryos (Corriero et al, 2007;Krysko et al, 2008).…”

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confidence: 99%

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Increased melanomacrophage centres in the liver of reproductively dysfunctional female greater amberjack Seriola dumerili (Risso, 1810)

Passantino

Zupa

Pousis

et al. 2020

Journal of Fish Diseases

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The greater amberjack Seriola dumerili is a new aquaculture fish that may display reproductive dysfunctions. During extensive follicular atresia, which is a common reproductive dysfunction in females during vitellogenesis, part of the reabsorbed yolk returns to the liver to be metabolized and recycled. Melanomacrophage centres (MMCs) are aggregates of macrophage‐like cells that play a role in the destruction, detoxification and recycling of endogenous and exogenous materials, and have been associated with systemic stress. Wild and captive‐reared greater amberjack were sampled in the Mediterranean Sea during two different phases of the reproductive cycle. The liver of reproductively dysfunctional captive‐reared females sampled during the spawning season showed a high density of both MMCs and apoptotic cells. A weak liver anti‐cytochrome P450 monooxygenase 1A immunoreactivity was observed, suggesting that the examined fish were not exposed to environmental pollutants. We propose that the observed increase in MMCs and apoptosis in captive‐reared fish was related to the hepatic overload associated to the metabolism of yolk proteins reabsorbed during extensive follicular atresia. Since follicular atresia is a frequent physiological and pathological event in teleosts, we suggest that the reproductive state should be always assessed when MMCs are used as markers of exposure to stress or pollutants.

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Inbreeding depression in sperm quality in a critically endangered amphibian

Hinkson

Poo

2020

Zoo Biology

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Small, isolated populations often experience increased inbreeding and decreased heterozygosity, which increases the potential risk of inbreeding depression.The relationship between inbreeding and sperm health is well-documented in a variety of taxa, but has yet to be explored in amphibians. The dusky gopher frog, Lithobates sevosus, is a critically endangered species with years of documented inbreeding and low genetic variability as a consequence of isolation and population size reduction. This study investigated the effects of inbreeding on sperm quality in captive L. sevosus using an outbred, sister species (Lithobates pipiens) as a standard for comparison. We found L. sevosus to have severely reduced sperm quality in terms of total motility, forward progressive motility, concentration, and viability. Additionally, we observed a significant, negative relationship between total sperm motility and mean kinship within captive-bred individuals. These data serve to enhance our understanding of the role inbreeding plays in amphibians, and to provide valuable insight into new risk factors declining amphibian populations may face. K E Y W O R D S assisted reproductive technologies, captive breeding, dusky gopher frog, fertility, Lithobates sevosus

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Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810)1

Cited by 13 publications

References 50 publications

Applying cryopreservation to anuran conservation biology

Applying cryopreservation to anuran conservation biology

Increased melanomacrophage centres in the liver of reproductively dysfunctional female greater amberjack Seriola dumerili (Risso, 1810)

Inbreeding depression in sperm quality in a critically endangered amphibian

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