Mitochondria as a Target for the Cardioprotective Effects of Cydonia oblonga Mill. and Ficus carica L. in Doxorubicin-Induced Cardiotoxicity

Gholami, Saman; Hosseini, Mir-Jamal; Jafari, Leila; Omidvar, Fatemeh; Kamalinejad, Mohammad; Mashayekhi, Vida; Hosseini, Seyed Nejat; Kardan, Azin; Pourahmad, Jalal; Eskandari, Mohammad Reza

doi:10.1055/s-0043-101824

Cited by 21 publications

(10 citation statements)

References 38 publications

(38 reference statements)

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“…Under our experimental conditions, Dox (0.5 and 1 μM for 24 hours) caused cytotoxicity on H9c2 cells, as indicated by cell mass loss, decreased metabolic viability, loss of ΔΨm, increased levels of mitochondrial superoxide anion, and increased caspase-3 and caspase-9-like activity, consistent with our previous studies in the same model [62,63]. Our results demonstrated that pretreatment with both plant extracts (1 and 25 µg/mL) prevented Dox-induced cytotoxicity in H9c2 cells, especially at the highest concentration tested.…”

Section: Discussionsupporting

confidence: 91%

Afrostyrax lepidophyllus Mildbr. and Monodora myristica (Gaertn.) Dunal Extracts Decrease Doxorubicin Cytotoxicity on H9c2 Cardiomyoblasts

Moukette

Castelão-Baptista

Ferreira

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Ethnopharmacological Relevance. Doxorubicin (Dox) is an anthracycline antibiotic widely used in cancer treatment. Despite its antitumor efficacy, its clinical application is significantly limited because of its cardiotoxicity originated, among other factors, from pro-oxidant damage to cardiac mitochondria. Phytochemicals represent a potentially attractive strategy to mitigate Dox cardiotoxicity due to their antioxidant properties, with plant extracts used in traditional medicine often being ignored in terms of potential therapeutic uses. Aim of the Study. The present study aimed at investigating the protective effects of two native Cameroonian plants, Afrostyrax lepidophyllus Mildbr. (A. lepidophyllus) and Monodora myristica (Gaertn.) Dunal (M. myristica), against Dox-induced cytotoxicity on cultured H9c2 cardiomyoblast cells. Materials and Methods. Bark extracts of these plants (1 and 25 µg/mL) were added 3 hours before coincubating H9c2 cardiomyoblasts with Dox (0.5 and 1 µM) for 24 hours more. We measured cell mass and metabolic viability, mitochondrial transmembrane potential, superoxide anion content, and activity-like of caspase-3 and caspase-9 following treatment with the extracts and/or Dox. Also, selenium and vitamin C contents were measured in the plant extracts. Results. The results confirmed that Dox treatment decreased cell mass, mitochondrial membrane potential and metabolic viability, increased mitochondrial superoxide anion, and stimulated caspase-3 and caspase-9-like activities. Pretreatment of the cells with the plant extracts significantly inhibited Dox cytotoxicity, with more significant results at the higher concentration. Measurements of selenium and vitamin C in the extracts revealed higher concentration of both when compared with other Cameroonian spices. Conclusion. Both extracts of A. lepidophyllus and M. myristica were effective against Dox-induced cytotoxicity, most likely due to their content in antioxidants.

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Section: Discussionsupporting

confidence: 91%

Afrostyrax lepidophyllus Mildbr. and Monodora myristica (Gaertn.) Dunal Extracts Decrease Doxorubicin Cytotoxicity on H9c2 Cardiomyoblasts

Moukette

Castelão-Baptista

Ferreira

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…The final extract contained 24 % water. The extract were dissolved in distilled water at the desired concentrations just before use [21]. We chose a wide range of concentrations for the aqueous extract of C. moschata fruit in our pilot study.…”

Section: Methodsmentioning

confidence: 99%

Cytoprotective Effects of Pumpkin (Cucurbita Moschata) Fruit Extract against Oxidative Stress and Carbonyl Stress

et al. 2017

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Diabetes mellitus is a chronic endocrine disorder that is associated with significant mortality and morbidity due to microvascular and macrovascular complications. Diabetes complications accompanied with oxidative stress and carbonyl stress in different organs of human body because of the increased generation of free radicals and impaired antioxidant defense systems. In the meantime, reactive oxygen species (ROS) and reactive carbonyl species (RCS) have key mediatory roles in the development and progression of diabetes complications. Therapeutic strategies have recently focused on preventing such diabetes-related abnormalities using different natural and chemical compounds. Pumpkin () is one of the most important vegetables in the world with a broad-range of pharmacological activities such as antihyperglycemic effect. In the present study, the cytoprotective effects of aqueous extract of fruit on hepatocyte cytotoxicity induced by cumene hydroperoxide (oxidative stress model) or glyoxal (carbonylation model) were investigated using freshly isolated rat hepatocytes. The extract of (50 μg/ml) excellently prevented oxidative and carbonyl stress markers, including hepatocyte lysis, ROS production, lipid peroxidation, glutathione depletion, mitochondrial membrane potential collapse, lysosomal damage, and cellular proteolysis. In addition, protein carbonylation was prevented by in glyoxal-induced carbonyl stress. It can be concluded that has cytoprotective effects in oxidative stress and carbonyl stress models and this valuable vegetable can be considered as a suitable herbal product for the prevention of toxic subsequent of oxidative stress and carbonyl stress seen in chronic hyperglycemia.

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“…Total polyphenol content was determined by spectrophotometry, using gallic acid as the standard based on the Folin-Ciocalteu method as previously described (Gholami et al, 2017[ 16 ]).…”

Section: Methodsmentioning

confidence: 99%

Cancer chemoprevention by oleaster (Elaeagnus angustifoli L.) fruit extract in a model of hepatocellular carcinoma induced by diethylnitrosamine in rats

Amereh

Hatami

Shirazi

et al. 2017

EXCLI Journal; 16:Doc1046; ISSN 1611-2156

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Mitochondria as a Target for the Cardioprotective Effects of Cydonia oblonga Mill. and Ficus carica L. in Doxorubicin-Induced Cardiotoxicity

Cited by 21 publications

References 38 publications

Afrostyrax lepidophyllus Mildbr. and Monodora myristica (Gaertn.) Dunal Extracts Decrease Doxorubicin Cytotoxicity on H9c2 Cardiomyoblasts

Afrostyrax lepidophyllus Mildbr. and Monodora myristica (Gaertn.) Dunal Extracts Decrease Doxorubicin Cytotoxicity on H9c2 Cardiomyoblasts

Cytoprotective Effects of Pumpkin (Cucurbita Moschata) Fruit Extract against Oxidative Stress and Carbonyl Stress

Cancer chemoprevention by oleaster (Elaeagnus angustifoli L.) fruit extract in a model of hepatocellular carcinoma induced by diethylnitrosamine in rats

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