Mitigation of sterigmatocystin exposure in cattle by difructose anhydride III feed supplementation and detection of urinary sterigmatocystin and serum amyloid A concentrations

Abstract

Abstract. We evaluated the effects of supplementing cattle feed with difructose anhydride III (DFA III) by measuring urinary sterigmatocystin (STC) concentrations using 20 Japanese Black cattle aged 9–10 months from one herd. DFA III was supplemented for 2 weeks for 10 animals, and non-treated animals served as controls. The natural STC concentration in the dietary feed was 0.06 mg kg−1 (mixture of roughage and concentrate) at the beginning of the study (Day 0). The urine STC concentration was measured using liquid chromatography with tandem mass spectrometry 1 d prior to DFA III administration, 9 and 14 d thereafter, and 9 d following supplementation cessation, concomitant with the measurement of serum amyloid A (SAA). The number of heifers in which STC was detected in the urine was low (10 %) in the DFA III group compared to that (60 %) in the control group on Day 9. After 9 d following supplementation cessation (Day 23), STC concentrations were significantly lower (P=0.032) in the DFA III group than in the control group, although there was no difference in the number of heifers in which urinary STC was detected or in SAA concentrations between the two groups. Our findings demonstrate the effect of DFA III on reducing the urinary concentration of STC in Japanese Black cattle.

Measurement of serum short-chain fatty acid concentrations in cattle after oral administration of difructose anhydride III

“…In addition, treatment of such oligosaccharides was proposed to restore the impaired epithelial barrier functions in the small intestinal epithelial cells [ 8 , 28 ]. We have also reported the effects of DFA III supplementation on calf/cattle, where we showed that supplementing DFA III with milk replacer reduced the onset of diseases, such as fever and diarrhea, during the pre-weaning period [ 17 , 18 ], and the effect of supplementing DFA III on feed-related epithelial barrier functions to reduce the absorption of mycotoxins, such as ZEN and STC, from the intestinal tract by measuring their urinary concentrations [ 19 , 20 ]. As a stepping-stone to elucidate the effects of DFA III supplementation on cattle health and intestinal defense function, in this study, we aimed to confirm whether the addition of DFA (40 g/days) to the diet increased the production of fatty acids in the intestinal tract and caused a difference in the serum concentrations of SCFAs after absorption from the intestinal tract.…”

“…[ 19 ], Sasazaki et al . [ 20 ]. Briefly, JB female heifers from two beef herds (Herd 1: n = 10; Herd 2: n = 20; approximately 10 months old and weighing 250–300 kg) raised in Kagoshima Prefecture, Japan, were included in this experiment.…”

“…[ 19 ], Sasazaki et al . [ 20 ], although we performed blood sampling at the start of DFA III supplementation (day 0), on days 9 and 14 (day 14, i.e., on the last day of DFA III supplementation) after treatment initiation and on the last day of the experimental period on day 23, the blood samples collected on days 0, 9, and 14 were used for the measurement of SCFA concentrations in the present study during the DFA III-supplemented periods.…”

“…We previously reported the efficacy of DFA III supplementation as a prebiotic for the improvement of the health and intestinal microbiota in calves [ 17 , 18 ]. We showed that it protected the integrity of the intestinal barrier in cattle and can be used as an alternative to protect against chronic low-dose mycotoxin contamination from cattle diets [ 19 , 20 ]. A previous report with experimental animals (rats) indicated that cecal SCFA concentrations in the DFA III-supplemented group were higher than those in the non-supplemented group under the same feeding conditions [ 21 , 22 ].…”

Practical Application of Urinary Zearalenone Monitoring System for Feed Hygiene Management of a Japanese Black Cattle Breeding Herd—The Relationship between Monthly Anti-Müllerian Hormone and Serum Amyloid A Concentrations

“…Previously, we have established a urinary ZEN-monitoring system with ELISA for initial screening purposes, followed by LC-MS/MS validation to detect ZEN and its related metabolites; α-zearalenol (α-ZEL) and β-zearalenol (β-ZEL) as well as sterigmatocystin (STC), as both toxins might occur together in diets for Japanese cattle [10][11][12]. Additionally, we have reported that monitoring ZEN or STC levels in urine is not only a practical and useful way of evaluating and detecting the naturally contamination status of cattle herds, but also assessing the efficiency of mycotoxin adsorbents (MAs) supplemented in dietary feed to reduce intestinal absorption of mycotoxins [10,11,13,14].…”