Missense substitutions lethal to essential functions of EF-Tu

Phylogeny of the Enterobacteriaceae based on genes encoding elongation factor Tu and F-ATPase β-subunit

“…The sequences obtained in this study are comparable to enterobacterial sequences from other studies available in public databases (Abdulkarim et al, 1991;Amann et al, 1988b;Blattner et al, 1997;Hudson et al, 1981;Perna et al, 2001;Saraste et al, 1981). However, some degree of polymorphism was observed.…”

Phylogeny of the Enterobacteriaceae based on genes encoding elongation factor Tu and F-ATPase β-subunit

“…The sequences obtained in this study are comparable to enterobacterial sequences from other studies available in public databases (Abdulkarim et al, 1991;Amann et al, 1988b;Blattner et al, 1997;Hudson et al, 1981;Perna et al, 2001;Saraste et al, 1981). However, some degree of polymorphism was observed.…”

Functional Constraints on Replacing an Essential Gene with Its Ancient and Modern Homologs

“…Given the very high level of conservation of EF-Tu, it is possible that the nonviability of some foreign EF-Tus might be related to the alteration of just one or a few critically important residues. Indeed, many single-amino-acid substitutions in EF-Tu have been shown to generate protein variants that do not support viability ( 50 ), including one single-amino-acid substitution in EF-Tu that permits ternary complex formation but abolishes translation activity by preventing ternary complex interaction with the ribosome ( 46 ). To facilitate an assessment of the amino acid differences between viable and nonviable EF-Tus, their amino acid sequences were aligned ( Fig.…”

A single amino acid substitution in elongation factor Tu disrupts interaction between the ternary complex and the ribosome

“…The selection of the mutation studied here, tufB414, has been described previously (1). Briefly (23) by cotransduction with the linked marker zhb-736::TnlO.…”

“…The selection of the mutation studied here, tufB414, has been described previously (1). Briefly, beginning with strain TH488 (which carries a kirromycin-resistant tufA allele and a wild-type kirromycin-sensitive tufB gene and is thus sensitive to the antibiotic), mutants resistant to kirromycin were selected.…”

Phylogeny of the Enterobacteriaceae based on genes encoding elongation factor Tu and F-ATPase β-subunit

“…The sequences obtained in this study are comparable to enterobacterial sequences from other studies available in public databases (Abdulkarim et al, 1991;Amann et al, 1988b;Blattner et al, 1997;Hudson et al, 1981;Perna et al, 2001;Saraste et al, 1981). However, some degree of polymorphism was observed.…”

Functional Constraints on Replacing an Essential Gene with Its Ancient and Modern Homologs

“…Given the very high level of conservation of EF-Tu, it is possible that the nonviability of some foreign EF-Tus might be related to the alteration of just one or a few critically important residues. Indeed, many single-amino-acid substitutions in EF-Tu have been shown to generate protein variants that do not support viability ( 50 ), including one single-amino-acid substitution in EF-Tu that permits ternary complex formation but abolishes translation activity by preventing ternary complex interaction with the ribosome ( 46 ). To facilitate an assessment of the amino acid differences between viable and nonviable EF-Tus, their amino acid sequences were aligned ( Fig.…”

A single amino acid substitution in elongation factor Tu disrupts interaction between the ternary complex and the ribosome

“…The selection of the mutation studied here, tufB414, has been described previously (1). Briefly (23) by cotransduction with the linked marker zhb-736::TnlO.…”

“…The selection of the mutation studied here, tufB414, has been described previously (1). Briefly, beginning with strain TH488 (which carries a kirromycin-resistant tufA allele and a wild-type kirromycin-sensitive tufB gene and is thus sensitive to the antibiotic), mutants resistant to kirromycin were selected.…”

Phylogeny of the Enterobacteriaceae based on genes encoding elongation factor Tu and F-ATPase β-subunit

“…The sequences obtained in this study are comparable to enterobacterial sequences from other studies available in public databases (Abdulkarim et al, 1991;Amann et al, 1988b;Blattner et al, 1997;Hudson et al, 1981;Perna et al, 2001;Saraste et al, 1981). However, some degree of polymorphism was observed.…”

Functional Constraints on Replacing an Essential Gene with Its Ancient and Modern Homologs

“…Given the very high level of conservation of EF-Tu, it is possible that the nonviability of some foreign EF-Tus might be related to the alteration of just one or a few critically important residues. Indeed, many single-amino-acid substitutions in EF-Tu have been shown to generate protein variants that do not support viability ( 50 ), including one single-amino-acid substitution in EF-Tu that permits ternary complex formation but abolishes translation activity by preventing ternary complex interaction with the ribosome ( 46 ). To facilitate an assessment of the amino acid differences between viable and nonviable EF-Tus, their amino acid sequences were aligned ( Fig.…”

A single amino acid substitution in elongation factor Tu disrupts interaction between the ternary complex and the ribosome

“…The selection of the mutation studied here, tufB414, has been described previously (1). Briefly (23) by cotransduction with the linked marker zhb-736::TnlO.…”

“…The selection of the mutation studied here, tufB414, has been described previously (1). Briefly, beginning with strain TH488 (which carries a kirromycin-resistant tufA allele and a wild-type kirromycin-sensitive tufB gene and is thus sensitive to the antibiotic), mutants resistant to kirromycin were selected.…”