Missense mutation in glutathione-S-transferase M1 gene is associated with sperm motility and ATP content in frozen-thawed semen of Holstein-Friesian bulls

Hering, Dorota; Lecewicz, Marek; Kordan, Władysław; Majewska, Anna; Kamiński, Stanisław

doi:10.1016/j.anireprosci.2015.06.001

Cited by 13 publications

(8 citation statements)

References 29 publications

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“…GST enzymes are also important in protecting sperm from cryopreservation of semen, as this process can produce large amounts of ROS. In freeze-thawed bull semen, a C/G missense mutation in rs135955605 within the GSTM1 gene is associated with cellular ATP content and total sperm motility [ 33 ]; therefore, genetic variations in GSTs may affect male fecundity, including sperm quality and the outcomes of semen cryopreservation.…”

Section: Genetic Variations In Antioxidant Genes Associated With Mmentioning

confidence: 99%

Variations in Antioxidant Genes and Male Infertility

Huang

2015

BioMed Research International

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Oxidative stress and reactive oxygen species (ROS) are generated from both endogenous and environmental resources, which in turn may cause defective spermatogenesis and male infertility. Antioxidant genes, which include catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST), nitric oxide synthase (NOS), nuclear factor erythroid 2-related factor 2 (NRF2), and superoxide dismutase (SOD), play important roles in spermatogenesis and normal sperm function. In this review, we discuss the association between variations in major antioxidant genes and male infertility. Numerous studies have suggested that genetic disruption or functional polymorphisms in these antioxidant genes are associated with a higher risk for male infertility, which include low sperm quality, oligoasthenoteratozoospermia, oligozoospermia, and subfertility. The synergistic effects of environmental ROS and functional polymorphisms on antioxidant genes that result in male infertility have also been reported. Therefore, variants in antioxidant genes, which independently or synergistically occur with environmental ROS, affect spermatogenesis and contribute to the occurrence of male infertility. Large cohort and multiple center-based population studies to identify new antioxidant genetic variants that increase susceptibility to male infertility as well as validate its potential as genetic markers for diagnosis and risk assessment for male infertility for precise clinical approaches are warranted.

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Section: Genetic Variations In Antioxidant Genes Associated With Mmentioning

confidence: 99%

Variations in Antioxidant Genes and Male Infertility

Huang

2015

BioMed Research International

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“…One of the important antioxidant enzymes in sperm is GST, and its inhibition results in damage to the cell membrane. As a result, it is associated with decreasing motility, inhibiting acrosome reaction, and reducing its ability to fertilize the oocyte (30). In this experiment, an increase in AA leads to depletion of glutathione reserves and a loss of balance between the oxidant and antioxidant system and provides conditions for the reduction of antioxidant reserves and the creation of lipid peroxidation process.…”

Section: Discussionmentioning

confidence: 88%

The effect of acrylamide on mitochondrial membrane potential and glutathione extraction in human spermatozoa: A laboratory study

Omidi

Piravar

Ramezani

2020

IJRM

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Background: Acrylamide (AA) is a compound used in the industrial production of polyacrylamide. AAs affects by creating oxidative stress. It produces reactive oxygen species and leads to lipid peroxide. Lipid peroxidation in the cell membrane is one of the most important oxidations in the sperm, which can disrupt the fluidity and permeability of cell membranes and damage all cells. Objective: To investigate the different concentrations of AA on human sperm parameters based on the World Health Organization standard and its impact on mitochondrial membrane potential and sperm glutathione levels. Materials and Methods: In this laboratory study, we examined the different concentrations of AA on human sperm parameters based on the World Health Organization standard and its impact on mitochondrial membrane potential by flow cytometry and sperm glutathione levels by ELISA assay. Results: The results were reported as the mean fluorescence intensity of JC and the index was observed to decrease following the effect of AA in mitochondrial membrane potential (Δ Ψm). The results of ELISA test to study the level of intracellular glutathione showed that with the increase in the concentration of AA exposed to sperms, there was a significant reduction in the level of intracellular glutathione. Conclusion: AA destroys the sperm membrane integrity under apoptotic and oxidative inductions with a negative impact on mitochondrial function and antioxidative enzyme in sperm such as glutathione. Key words: Acrylamides, Spermatozoa, Mitochondrial membrane potential, Glutathione S-Transferase.

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“…Both of these glutathione‐related‐enzymes proteins are likely associated with maintaining the oxidative balance and protection of sperm function. In B. taurus bulls a C/G missense mutation within the GST(M1) gene has been shown to affect both sperm motility and ATP content postthawing (Hering, Lecewicz, Kordan, Majewska, & Kaminski, 2015). Studies indicated that GPX5 in the lumen of the epididymis assists with optimizing the oxidative stress level in the fluid surrounding the maturing spermatozoa, likely affecting chromatin condensation through protamine disulfate bindings or DNA fragmentation (review by Champroux, Torres‐Carreira, Gharagozloo, Drevet, & Kocer, 2016).…”

Section: Discussionmentioning

confidence: 99%

Effects of increased scrotal temperature on semen quality and seminal plasma proteins in Brahman bulls

Boe-Hansen

Rêgo²,

Satake

et al. 2020

Molecular Reproduction Devel

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Environmental temperature has effects on sperm quality with differences in susceptibility between cattle subspecies and breeds, but very little is known about the seminal plasma protein (SPP) changes resulting from testicular heat stress. Scrotal insulation (SI) for 48 hr was applied to Brahman (Bos indicus) bulls. Semen was collected at 3-day intervals from before, until 74 days post-SI. The changes in sperm morphology and motility following SI were comparable to previously reported and differences were detected in measures of sperm chromatin conformation as early as 8 days post-SI. New proteins spots, in the SPP two-dimensional (2-D) gels, were apparent when comparing pre-SI with 74 days post-SI, and SPP identified as associated with mechanisms of cellular repair and protection. Similar trends between 2-D gel and Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH -MS) data was observed, with SWATH-MS able to quantify individual SPP that otherwise were not resolved on 2-D gel. The SPP assessment at peak sperm damage (21-24 days) showed a significant difference in 29 SPP (adjusted p < .05), and identified six proteins with change in abundance in the SI group. In conclusion both spermatozoa and SPP composition of bulls are susceptible to temperature change incurred by SI, and SPP markers for testicular heat insults may be detected. K E Y W O R D Schromatin structure, MS-based proteomics, scrotal insulation, testicular stress Note: Period 1-impact on epididymal maturation (2-11 days post-SI), Period 2-impact on spermiogenesis and meiosis (14-41 days post-SI), Period 3-impact on spermatocytogenesis (44-62 days post-SI), and Period 4-impact after one full wave of spermatogenesis (65-74 days post-SI); where Day 0 represents initiation of the 48 hr period of SI. The data are presented as means ± SE for bulls with applied SI for 48 hr (n = 3) and control (n = 3). The PNS is further differentiated into the proportion of spermatozoa with HD, D, MPD, and TD.

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Missense mutation in glutathione-S-transferase M1 gene is associated with sperm motility and ATP content in frozen-thawed semen of Holstein-Friesian bulls

Cited by 13 publications

References 29 publications

Variations in Antioxidant Genes and Male Infertility

Variations in Antioxidant Genes and Male Infertility

The effect of acrylamide on mitochondrial membrane potential and glutathione extraction in human spermatozoa: A laboratory study

Effects of increased scrotal temperature on semen quality and seminal plasma proteins in Brahman bulls

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