2010
DOI: 10.1016/j.humimm.2009.11.003
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Misleading de novo detection of serum anti–HLA-A3 antibodies in kidney recipients having received ATG before transplantation

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Cited by 11 publications
(6 citation statements)
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References 23 publications
(28 reference statements)
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“…No information is given about against which class (I and/or II) were the detected anti-HLA antibodies. The main issue here is if this association results from a laboratory interference of a xeno-antibody present in ATG preparation [13] with no foreseeable effect on the graft or if it results from an imbalance between T- and B-cell populations with a stronger depletion effect in the former (including regulatory T-cells) allowing for humoral responses to evolve [14]. Our results are in accordance with the first proposition, since we reported only 2 graft losses in the ATG induced group and no significant difference was detected in acute rejection between patients with and without ATG induction.…”
Section: Discussionmentioning
confidence: 99%
“…No information is given about against which class (I and/or II) were the detected anti-HLA antibodies. The main issue here is if this association results from a laboratory interference of a xeno-antibody present in ATG preparation [13] with no foreseeable effect on the graft or if it results from an imbalance between T- and B-cell populations with a stronger depletion effect in the former (including regulatory T-cells) allowing for humoral responses to evolve [14]. Our results are in accordance with the first proposition, since we reported only 2 graft losses in the ATG induced group and no significant difference was detected in acute rejection between patients with and without ATG induction.…”
Section: Discussionmentioning
confidence: 99%
“…Since Thymoglobuline is produced by immunization with human thymocytes of several individuals, multiple HLA class I and II specificities can be present. In the case of Gravalon (immunization with a single Jurkat cell line expressing HLA‐A3, A32, B7, B35), the specificities are better defined, and thus, false‐positive results are easier to identify . In addition, these rabbit HLA antibodies compete with human HLA antibodies for binding to the SAB and can therefore diminish the MFI signal.…”
Section: Technical Challenges and Limitationsmentioning
confidence: 99%
“…Such ATG antibodies are more likely to bind to host cells and tissues and therefore should not act like DSA. However, evidence for ATG antibodies that specifically react with a certain HLA type has been reported, and this phenomenon certainly merits further investigation . In a previous study, we have found a high homogeneity between different batches of thymoglobulin .…”
mentioning
confidence: 67%