Miscibility of choline-substituted polyphosphazenes with PLGA and osteoblast activity on resulting blends

Weikel, Arlin L.; Owens, Steven G.; Morozowich, Nicole L.; Deng, Meng; Nair, Lakshmi S.; Laurencin, Cato T.; Allcock, Harry R.

doi:10.1016/j.biomaterials.2010.07.094

Cited by 38 publications

(37 citation statements)

References 34 publications

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“…The M w / M n is known as the molecular weight polydispersity index (PI), which is characteristic of the width of the polymer molecular weight distribution. 25,26 GPC can detect changes in the M w and PI of biomaterials during degradation. It can be seen in Figure 4 Polylactic acid polymer degradation appeared to follow first order kinetics.…”

Section: Sensitive Time Window and Dose Effects During The Sensitive mentioning

confidence: 99%

Degradation and Bio-Safety Evaluation of mPEG-PLGA-PLL Copolymer-Prepared Nanoparticles

Sun

Wang

et al. 2015

J. Phys. Chem. C

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Studies have shown that monomethoxy(polyethylene glycol)−poly(D,Llactic-co-glycolic acid)−poly(L-lysine) (mPEG-PLGA-PLL)-prepared nanoparticles (NPs) are promising drugs carriers, with good drug loading and delivery performance. To further promote the use of this material in clinical applications, its degradation and biosafety were evaluated. This paper describes degradation studies and biosafety evaluations of different block composition ratios (LA/GA = 60/40, 70/30, and 80/20) of the main material, PLGA, for mPEG-PLGA-PLL (PEAL) NPs. The degradation of PEAL NPs was studied by characterizing the change in molecular weight, the chemical composition, and the degradation rate in addition to the pH value, the particle size, the zeta potential, and the lactic acid and lysine contents in degradation solutions by transmission electron microscopy (TEM), gel permeation chromatography (GPC), and 1 H NMR. The results show that with prolonged degradation time, the pH, particle size, zeta potential, and molecular weight were reduced and that the lactic acid and lysine contents and the molecular weight distribution were increased. 1 H NMR demonstrated that the hydrolysis rate for glycolic units was faster than those for lactic acid and lysine units. The degradation rate of NPs in pH 7.4 PBS was faster than that in pH 5.0 PBS. The degradation rate of PEAL NPs increased as the LA/GA increased from LA/GA = 60/40 to 80/20. Investigations of intracellular protein synthesis, lactate dehydrogenase (LDH) release, 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining and reactive oxygen species (ROS) content in Huh7, L02, and RAW 264.7 cells showed that the PEAL NPs had no effect on protein synthesis or cell membrane integrity and did not induce chromatin agglutination. Although the ROS content was slightly concentration-dependent and time-dependent, the change in content was minimal and diffusely distributed within the cell. After THP-1 cells were induced to differentiate into macrophages, a subsequent incubation with 5 mM PEAL NPs for 24 h did not significantly induce the macrophage release of IL-1β, TNF-α, and TGF-β1 compared with the negative control. Embryos that had their chorion removed were coincubated with PEAL NPs to determine if there were any effects on embryonic development. It is known that zebrafish embryos at 10−24 h post-fertilization (hpf) are most sensitive to PEAL NPs. Zebrafish embryos treated with different concentrations of PEAL NPs within this sensitive time frame demonstrated that PEAL NPs have a high level of biosafety. Our work demonstrates that PEAL NPs are safe candidates for use as biodegradable carriers for drug and gene delivery.

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Section: Sensitive Time Window and Dose Effects During The Sensitive mentioning

confidence: 99%

Degradation and Bio-Safety Evaluation of mPEG-PLGA-PLL Copolymer-Prepared Nanoparticles

Sun

Wang

et al. 2015

J. Phys. Chem. C

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“…A previous study has capitalized on these hydrogen-bonding capabilities to blend of polyphosphazenes with poly(lactide-co-glycolide) (PLAGA)[5, 18, 19, 22, 23, 41, 43, 48, 69, 85]. In that work, it was discovered that miscible blends can be formed between Polyphosphazenes that contain glycine ethyl ester side groups and PLGA with a lactic to glycolic acid ratio of 50:50 [85]. Miscible blends can also be obtained blending Alanine ethyl ester phosphazene polymers with PLAGA (50:50).…”

Section: Biodegradable Polyphosphazene (Bio-functionalized) and Its Bmentioning

confidence: 99%

“…Miscible blends can also be obtained blending Alanine ethyl ester phosphazene polymers with PLAGA (50:50). However, the presence of the steric methyl group at the α-carbon places some difficulties on the formation of hydrogen bonding with PLAGA[22, 27, 48, 69, 85, 86]. …”

Section: Biodegradable Polyphosphazene (Bio-functionalized) and Its Bmentioning

confidence: 99%

Biodegradable Polyphosphazene-Based Blends for Regenerative Engineering

Ogueri

Ivirico

Nair

et al. 2017

Regen. Eng. Transl. Med.

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The occurrence of musculoskeletal tissue injury or disease and the subsequent functional impairment is at an alarming rate. It continues to be one of the most challenging problems in the human health care. Regenerative engineering offers a promising transdisciplinary strategy for tissues regeneration based on the convergence of tissue engineering, advanced materials science, stem cell science, developmental biology and clinical translation. Biomaterials are emerging as extracellular-mimicking matrices designed to provide instructive cues to control cell behavior and ultimately, be applied as therapies to regenerate damaged tissues. Biodegradable polymers constitute an attractive class of biomaterials for the development of scaffolds due to their flexibility in chemistry and the ability to be excreted or resorbed by the body. Herein, the focus will be on biodegradable polyphosphazene-based blend systems. The synthetic flexibility of polyphosphazene, combined with the unique inorganic backbone, has provided a springboard for more research and subsequent development of numerous novel materials that are capable of forming miscible blends with poly (lactide-co-glycolide) (PLAGA). Laurencin and co-workers has demonstrated the exploitation of the synthetic flexibility of Polyphosphazene that will allow the design of novel polymers, which can form miscible blends with PLAGA for biomedical applications. These novel blends, due to their well-tuned biodegradability, and mechanical and biological properties coupled with the buffering capacity of the degradation products, constitute ideal materials for regeneration of various musculoskeletal tissues. Lay Summary Regenerative engineering aims to regenerate complex tissues to address the clinical challenge of organ damage. Tissue engineering has largely focused on the restoration and repair of individual tissues and organs, but over the past 25 years, scientific, engineering, and medical advances have led to the introduction of this new approach which involves the regeneration of complex tissues and biological systems such as a knee or a whole limb. While a number of excellent advanced biomaterials have been developed, the choice of biomaterials, however, has increased over the past years to include polymers that can be designed with a range of mechanical properties, degradation rates, and chemical functionality. The polyphosphazenes are one good example. Their chemical versatility and hydrogen bonding capability encourages blending with other biologically relevant polymers. The further development of Polyphosphazene-based blends will present a wide spectrum of advanced biomaterials that can be used as scaffolds for regenerative engineering and as well as other biomedical applications.

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“…solution of PMMA and the resulting mixture was stirred for 24 h. Then resulting solution of two polymers was allowed to stand for 1 hour to conform the miscibility of polymers [41].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Sustained release of hydrophilic drug from polyphosphazenes/poly(methyl methacrylate) based microspheres and their degradation study

Akram

Khalid

et al. 2016

Materials Science and Engineering: C

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Miscibility of choline-substituted polyphosphazenes with PLGA and osteoblast activity on resulting blends

Cited by 38 publications

References 34 publications

Degradation and Bio-Safety Evaluation of mPEG-PLGA-PLL Copolymer-Prepared Nanoparticles

Degradation and Bio-Safety Evaluation of mPEG-PLGA-PLL Copolymer-Prepared Nanoparticles

Biodegradable Polyphosphazene-Based Blends for Regenerative Engineering

Sustained release of hydrophilic drug from polyphosphazenes/poly(methyl methacrylate) based microspheres and their degradation study

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