Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers

Abstract

Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker systems such as: inter simple sequence repeats (ISSRs), random amplified polymorphic DNA (RAPD), and by estimation of ploidy level based on flow cytometry. As a result, only one ISSR primer (ISSR1) and three RAPD primers (RAPD1, RAPD2, RAPD4) were required to identify all genotypes. Moreover, the use of the above mentioned molecular markers enable the proper species recognition of the interspecific hybrid M. × giganteus “Floridulus,” which has been previously mislabeled as M. floridulus. The highest genetic similarity coefficient (0.94) was observed between M. × giganteus clones, which indicates that the genetic diversity within this species was very low. Whereas M. sinensis genotypes represented a relatively wide diversity with similarity coefficient of 0.58. Cluster analysis using UPGMA grouped the 18 accessions in three clusters according to species affiliation including relabeled M. × giganteus “Floridulus,” which proved to be closely related to M. × giganteus. Similar groupings were evident in the PCoA analysis.

Comparison of the genome size, endoreduplication, and ISSR marker polymorphism in eight Lotus (Fabaceae) species

“…Specific bands enabled the distinction even between very closely related species like L. japonicus and L. burttii, or L. corniculatus and L. tenuis. It confirmed the usefulness of ISSR markers as reported in previous studies, e.g., on Dendrobium (Wang et al, 2009), Ocimum (Chen et al, 2013;Rewers and Jedrzejczyk, 2016), and Miscanthus (Cichorz et al, 2014). Most of the primers tested here for Lotus revealed polymorphism between species, and three of them (ISSR-1, ISSR-24, and ISSR-30) also between accessions of the same species of different origin; therefore, they are recommended for identification of accessions of this genus.…”

Metal accumulation and shoot yield of Miscanthus×giganteus growing in contaminated agricultural soils: Insights into agronomic practices

“…Moreover, as a hybrid species reproducing solely by rhizome propagation, there could be little room for genetic variability among the studied cultivars. Indeed, lack or very low genetic variability and diversity among European and North American cultivated M. Â giganteus was evidenced (Cichorz et al, 2014;Yook et al, 2014).…”

Miscanthus: Inter- and Intraspecific Genome Size Variation Among M. × Giganteus, M. Sinensis, M. Sacchariflorus Accessions

“…species available in the field collection at the PBAI -NRI (Plant Breeding and Acclimatization Institute -National Research Institute, Bydgoszcz, Poland) were used (Cichorz et al, 2014 ) was chopped simultaneously with a fragment of a leaf of internal standard with a sharp razor blade in a plastic Petri dish containing 1 cm 3 of nuclei-isolation buffer (0.1 M Tris, 2.5 mM MgCl 2 ·6H 2 O, 85 mM NaCl, 0.1% v/v Triton X-100; pH 7.0), supplemented with PI (50 μg·cm ). Pisum sativum 'Set' (2C = 9.11 pg; Sliwinska et al, 2005) was used as an internal standard for all the samples except M. sinensis 'Goliath', where indirect internal standard, M. sinensis 'Malepartus' (2C = 5.52 pg; this work), was used.…”