“…The most important limitation is the low specificity of these small RNA molecules on targets, as one microRNA could inhibit several mRNAs, leading to a high theoretical risk of off‐target effects. For example, miR‐9 is predicted to target 1377, 545, and 683 mRNAs using TargetScan7.1 (Agarwal, Bell, Nam & Bartel, 2015), miRDB (Wong & Wang, 2015) and PicTar (Krek et al., 2005), respectively; to date, up to 360 validated targets are listed in miRTarBase (Chou et al., 2016). Therefore, it will be imperative to evaluate the off‐target effects of this miRNA approach or others, in silico , in vitro in cell models and in vivo in animals before progressing to humans.…”