MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance

Zhang, Chunren; Yu, Chuyi; Lin, Zengxian; Pan, Haixia; Li, Kunyin; Ma, Hongxia

doi:10.1007/s00404-020-05730-z

Cited by 13 publications

(5 citation statements)

References 44 publications

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“…In their study of key genes in resveratrol-induced apoptosis in porcine ovarian granulosa cells, Zhang et al found that the pathways signi cantly enriched were all associated with hormone secretion and metabolic pathways [26]. Zhang et al studied miRNA expression spectra in the ovary of rats with insulin resistance polycystic ovary syndrome (PCOS) and found that the predicted target genes correlated with metabolism pathways and that metabolism pathways were the richest pathways in KEGG enrichment analysis, which is consistent with our ndings [27]. In contrast, Su study of genes and their associated signalling pathways before and after primordial follicle initiation in mice found that differential target genes involved in ovarian follicle development were more enriched in the MAPK signalling pathway [28].…”

Section: Discussionsupporting

confidence: 88%

Analysis of the Resistance of Small Peptides from Periplaneta americana to Hydrogen Peroxide-induced Apoptosis in KGN Cells based on High-throughput miRNA Sequencing

Jiang,

Sun,

Tang

et al. 2023

Preprint

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Background: Apoptosis of ovarian granulosa cells can promote abnormal atresia folliculin and decrease women's reproductive function. MiRNA plays an essential molecular signal-regulating function in ovarian functional development, animal reproductive performances and follicular development. The purpose of this study is to investigate the miRNA molecules and action pathways during the resistance of small peptides from Periplaneta americana (SPPA) resistance to peroxide-induced ovarian granulosa cells (KGN cells) apoptosis. Results: The study found that 162 differentially expressed miRNAs were identified in the blank control group, H2O2 group and H2O2+SPPA group. The intersection of miRNAs between the three groups was determined, through which 13 differentially expressed miRNAs were identified. Target gene prediction of differentially expressed miRNAs yielded 4828 target genes. Finally, GO and KEGG analysis were performed on 4828 target genes. The target genes primarily engaged in transcription, DNA template, negative transcriptional regulation of RNA polymerase II promoter, negative regulation of cell proliferation, protein binding, metabolic pathways, and mitogen-activated protein kinase signalling pathways. After qRT-PCR verification, expression trends of miR-103a-3p and miR-214 were consistent with sequencing results. Conclusions: Critical miRNAs have been identified as miR-103a-3p and miR-214 in SPPA resistance to peroxide-induced KGN apoptosis.

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Section: Discussionsupporting

confidence: 88%

Analysis of the Resistance of Small Peptides from Periplaneta americana to Hydrogen Peroxide-induced Apoptosis in KGN Cells based on High-throughput miRNA Sequencing

Jiang,

Sun,

Tang

et al. 2023

Preprint

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“…miR-33b-5p is overexpressed in the ovarian tissue of insulin-resistant PCOS rats and therefore may play an important role in the development of insulin resistance in patients with PCOS (35). Another study found that miR-3585-5p and miR-30-5p were significantly upregulated and miR-146-5p was downregulated in the ovaries of rats with PCOS and insulin resistance (36). There are many changes in the expression of miRNA in the follicular fluid of patients with PCOS, and miR-127-3p has been found to be associated with insulin resistance (37).…”

Section: Discussionmentioning

confidence: 99%

Differential expression of microRNA in the serum of patients with polycystic ovary syndrome with insulin resistance

Huang¹,

Ji²,

Ye³

et al. 2022

Ann Transl Med

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Background: Polycystic ovary syndrome (PCOS) is the most common reproductive endocrine disease in women of childbearing age, and insulin resistance is an important etiological mechanism in PCOS. This study revealed the microRNA (miRNA) expression profile of PCOS with insulin resistance and explored the potential biological functions of differentially expressed miRNA.Methods: A total of 76 patients with PCOS and 30 normal healthy women were recruited in the gynecological clinic of the Second Hospital of Tianjin Medical University. We divided the patients with PCOS into a group with insulin resistance (n=46) and a group without insulin resistance (n=30). Peripheral venous serum samples from each group were used for deep sequencing to identify differentially expressed miRNAs. Hierarchical clustering heat maps were used to show differences in miRNA expression. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and target gene network databases were used to explore the potential target genes of differentially expressed miRNAs and to analyze their specific biological functions.Results: A case-control analysis found that the levels of body mass index (BMI), prolactin (PRL), total testosterone (T), fasting blood glucose (FBG), and fasting insulin (INS) in patients with PCOS were higher than those in healthy controls. High BMI, high blood sugar, and hyperinsulinemia were more significant in the PCOS with insulin resistance group than without insulin resistance group. Among the patients with PCOS, miR-122-5p was found to have more significant differences in the PCOS with insulin resistance group. GO and KEGG pathway analysis showed that the identified miRNAs were involved in the regulation of different biological processes, such as signal transduction, negative regulation of GTPase activity, chloride channel complex. The predicted target genes were related to the citrate cycle (TCA cycle) and the biosynthesis of mucin-type O-glycans.Conclusions: Our research demonstrated the use of miRNAs as new biomarkers for the diagnosis, treatment and presented a new strategy to lessen the symptoms of PCOS with insulin resistance.

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“…We have identi ed micro-RNA species has-miR-21-5p, has-miR-335-5p, has-miR-146a-5p and has-miR-143-5p shared by the hub genes. These micro-RNAs have been linked to the development of PCOS [49][50][51] .…”

Section: Discussionmentioning

confidence: 99%

Discovering common pathogenetic processes between SARS-CoV-2 infection and polycystic ovary syndrome based on an integrated bioinformatics approach and experiment validation

Bai,

Zhang,

et al. 2024

Preprint

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The prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among polycystic ovary syndrome (PCOS) is significantly higher than in the general population. However, the mechanisms underlying this remain obscure. This study aimed to explore the mechanisms by identifying genetic signature of SARS-CoV-2 infection in PCOS. In the present study, a total of 27 common differentially expressed genes (DEGs) were selected for subsequent analyses. Functional analyses showed that immunity and hormone related pathways collectively participated in the development and progression of PCOS and SARS Cov-2 infection. Under these, 7 significant hub genes were identified, including S100A9, MMP9, TLR2, THBD, ITGB2, ICAM1, CD86 by using the algorithm in Cytoscape. Furthermore, hub genes expression was confirmed in validation set, PCOS clinical samples and mouse model. Immune microenvironment analysis with CIBERSORTx database demonstrated that the hub genes were significantly correlated with T cells, dendritic cells, mast cells, B cells, NK cells, eosinophils and positively correlated with immune scores. Among the hub genes, S100A9, MMP9, THBD, ITGB2, CD86 and ICAM1 exhibited preferable values as diagnostic makers for COVID-19 and PCOS. In addition, we established the interaction networks of ovary-specific genes, transcription factors, miRNAs, drugs, and chemical compounds with hub genes with NetworkAnalyst. This work uncovered the common pathogenesis and genetic signature of PCOS and SARS-CoV-2 infection, which might provide a theoretical basis and innovative ideas for further mechanistic research and drug discovery of the comorbidity of two diseases.

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MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance

Cited by 13 publications

References 44 publications

Analysis of the Resistance of Small Peptides from Periplaneta americana to Hydrogen Peroxide-induced Apoptosis in KGN Cells based on High-throughput miRNA Sequencing

Analysis of the Resistance of Small Peptides from Periplaneta americana to Hydrogen Peroxide-induced Apoptosis in KGN Cells based on High-throughput miRNA Sequencing

Differential expression of microRNA in the serum of patients with polycystic ovary syndrome with insulin resistance

Discovering common pathogenetic processes between SARS-CoV-2 infection and polycystic ovary syndrome based on an integrated bioinformatics approach and experiment validation

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