Analysis of the Resistance of Small Peptides from Periplaneta americana to Hydrogen Peroxide-induced Apoptosis in KGN Cells based on High-throughput miRNA Sequencing

Abstract

Background: Apoptosis of ovarian granulosa cells can promote abnormal atresia folliculin and decrease women's reproductive function. MiRNA plays an essential molecular signal-regulating function in ovarian functional development, animal reproductive performances and follicular development. The purpose of this study is to investigate the miRNA molecules and action pathways during the resistance of small peptides from Periplaneta americana (SPPA) resistance to peroxide-induced ovarian granulosa cells (KGN cells) apoptosis. Results: The study found that 162 differentially expressed miRNAs were identified in the blank control group, H2O2 group and H2O2+SPPA group. The intersection of miRNAs between the three groups was determined, through which 13 differentially expressed miRNAs were identified. Target gene prediction of differentially expressed miRNAs yielded 4828 target genes. Finally, GO and KEGG analysis were performed on 4828 target genes. The target genes primarily engaged in transcription, DNA template, negative transcriptional regulation of RNA polymerase II promoter, negative regulation of cell proliferation, protein binding, metabolic pathways, and mitogen-activated protein kinase signalling pathways. After qRT-PCR verification, expression trends of miR-103a-3p and miR-214 were consistent with sequencing results. Conclusions: Critical miRNAs have been identified as miR-103a-3p and miR-214 in SPPA resistance to peroxide-induced KGN apoptosis.