miRNAs as potential game-changers in head and neck cancer: Future clinical and medicinal uses

El-Mahdy, Hesham A.; Mohamadin, Ahmed M.; Abulsoud, Ahmed I.; Khidr, Emad Gamil; El-Husseiny, Ahmed A.; Ismail, Ahmed; Elsakka, Elsayed G.E.; Mokhlis, Hamada A.; El-Husseiny, Hussein M.; Doghish, Ahmed S.

doi:10.1016/j.prp.2023.154457

Cited by 48 publications

(4 citation statements)

References 252 publications

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“…LncRNAs are arranged in secondary and three-dimensional structures so they can function as RNA and or protein [5]. The identification of non-coding RNA, such as long non-coding RNA (lncRNA) and microRNA (miRNA), in the advancement of human diseases has broadened to encompass potential connections with various conditions [6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

The clinical significance of long non-coding RNAs MALAT1 and CASC2 in the diagnosis of HCV-related hepatocellular carcinoma

Golam,

Khalil,

Shaker

et al. 2024

PLoS ONE

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Background Globally, hepatocellular carcinoma (HCC) is the second most common cause of cancer-related death due to a lack of early predictive and/or diagnostic tools. Thus, research for a new biomarker is important. LncRNAs play a functional role in target gene regulation and their deregulation is associated with several pathological conditions including HCC. Objective This study aimed to explore the diagnostic potential of two LncRNAs MALAT1 and CASC2 in HCC compared to the routinely used diagnostic biomarker. Materials and methods The current study is a case-control study carried out at Fayoum University Hospital and conducted on 89 individuals. The study included three groups of 36 HCC patients on top of HCV(HCC/HCV), 33 HCV patients, and 20 healthy volunteers as a control group. All study subjects were subjected to radiological examinations. The determination of CBC was performed by the automated counter and liver function tests by the enzymatic method were performed. In addition, HCV RNA quantification and the expression level of two LncRNAs (MALAT1 and CASC2) were performed by qRT-PCR. Results The results revealed a statistically significant difference between study groups regarding liver function tests with a higher mean in HCC/HCV group. Also, serum MALAT1 significantly up-regulated in HCV (11.2±2.8) and HCC/HCV (4.56±1.4) compared to the control group. Besides, serum CASC2 levels in the HCV group were significantly upregulated (14.9±3.6), while, downregulated in the HCC group (0.16± 0.03). Furthermore, The ROC analysis for diagnostic efficacy parameters indicated that CASC2 has higher accuracy (94.6%) and sensitivity (97.2%) for HCC diagnosis than AFP with an accuracy of (90.9%), sensitivity (69.4%), and MALAT1 showed an accuracy of (56.9%), sensitivity (72.2%). Conclusion Our study results indicated that CASC2 is a promising biomarker and is considered better and could help in HCC diagnosis on top of HCV than MALAT1 and the routine biomarker AFP.

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Section: Introductionmentioning

confidence: 99%

The clinical significance of long non-coding RNAs MALAT1 and CASC2 in the diagnosis of HCV-related hepatocellular carcinoma

Golam,

Khalil,

Shaker

et al. 2024

PLoS ONE

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“…The basic goal of TE is to repair damaged tissues and replace them with new biological ones ( Gaspar et al, 2020 ; Lavrador et al, 2021 ; El-Husseiny et al, 2022c ; El-Husseiny et al, 2022d ; El-Husseiny et al, 2022f ; Sharun et al, 2023 ). Cell biology and biochemistry are required for this interdisciplinary process ( El-Husseiny et al, 2022d ; Doghish et al, 2023 ; El-Mahdy et al, 2023 ; Mady et al, 2023 ; Sharun et al, 2023 ). For clinical applications, clinical medical and material sciences investigations are also included ( Gaspar et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Stimuli-responsive hydrogels: smart state of-the-art platforms for cardiac tissue engineering

El-Husseiny

Mady

El-Dakroury

et al. 2023

Front. Bioeng. Biotechnol.

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Biomedicine and tissue regeneration have made significant advancements recently, positively affecting the whole healthcare spectrum. This opened the way for them to develop their applications for revitalizing damaged tissues. Thus, their functionality will be restored. Cardiac tissue engineering (CTE) using curative procedures that combine biomolecules, biomimetic scaffolds, and cells plays a critical part in this path. Stimuli-responsive hydrogels (SRHs) are excellent three-dimensional (3D) biomaterials for tissue engineering (TE) and various biomedical applications. They can mimic the intrinsic tissues’ physicochemical, mechanical, and biological characteristics in a variety of ways. They also provide for 3D setup, adequate aqueous conditions, and the mechanical consistency required for cell development. Furthermore, they function as competent delivery platforms for various biomolecules. Many natural and synthetic polymers were used to fabricate these intelligent platforms with innovative enhanced features and specialized capabilities that are appropriate for CTE applications. In the present review, different strategies employed for CTE were outlined. The light was shed on the limitations of the use of conventional hydrogels in CTE. Moreover, diverse types of SRHs, their characteristics, assembly and exploitation for CTE were discussed. To summarize, recent development in the construction of SRHs increases their potential to operate as intelligent, sophisticated systems in the reconstruction of degenerated cardiac tissues.

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“…The current scarcity of human organs and tissues appropriate for life-saving transplants is expected to persist in the future despite the great endeavors to close the donation gap [ 1 , 2 , 3 , 4 , 5 , 6 ]. Hence, it is highly crucial to introduce efficient alternatives to avoid the high incidence of deaths among patients that occur due to the long waiting lists for donor organs/tissues or due to receiving suboptimal treatments [ 7 , 8 , 9 , 10 , 11 ]. Animal-originated organs/tissues (xenotransplants) have been recently introduced as efficient and potential alternatives to solve this problem.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Bovine- and Porcine-Derived Decellularized Biomaterials: Promising Platforms for Tissue Engineering Applications

et al. 2023

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Animal-derived xenogeneic biomaterials utilized in different surgeries are promising for various applications in tissue engineering. However, tissue decellularization is necessary to attain a bioactive extracellular matrix (ECM) that can be safely transplanted. The main objective of the present study is to assess the structural integrity, biocompatibility, and potential use of various acellular biomaterials for tissue engineering applications. Hence, a bovine pericardium (BP), porcine pericardium (PP), and porcine tunica vaginalis (PTV) were decellularized using a Trypsin, Triton X (TX), and sodium dodecyl sulfate (SDS) (Trypsin + TX + SDS) protocol. The results reveal effective elimination of the cellular antigens with preservation of the ECM integrity confirmed via staining and electron microscopy. The elasticity of the decellularized PP (DPP) was markedly (p < 0.0001) increased. The tensile strength of DBP, and DPP was not affected after decellularization. All decellularized tissues were biocompatible with persistent growth of the adipose stem cells over 30 days. The staining confirmed cell adherence either to the peripheries of the materials or within their matrices. Moreover, the in vivo investigation confirmed the biocompatibility and degradability of the decellularized scaffolds. Conclusively, Trypsin + TX + SDS is a successful new protocol for tissue decellularization. Moreover, decellularized pericardia and tunica vaginalis are promising scaffolds for the engineering of different tissues with higher potential for the use of DPP in cardiovascular applications and DBP and DPTV in the reconstruction of higher-stress-bearing abdominal walls.

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miRNAs as potential game-changers in head and neck cancer: Future clinical and medicinal uses

Cited by 48 publications

References 252 publications

The clinical significance of long non-coding RNAs MALAT1 and CASC2 in the diagnosis of HCV-related hepatocellular carcinoma

The clinical significance of long non-coding RNAs MALAT1 and CASC2 in the diagnosis of HCV-related hepatocellular carcinoma

Stimuli-responsive hydrogels: smart state of-the-art platforms for cardiac tissue engineering

Comparison of Bovine- and Porcine-Derived Decellularized Biomaterials: Promising Platforms for Tissue Engineering Applications

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