miRNA-seq analysis in skeletal muscle of chicken and function exploration of miR-24-3p

Wu, Pengfei; He, Ming‐Liang; Zhang, Xinchao; Zhou, Kaizhi; Zhang, Tao; Xie, Kaizhou; Dai, Guojun; Wang, Jinyu; Wang, Xinglong; Zhang, Genxi

doi:10.1016/j.psj.2022.102120

Cited by 2 publications

(4 citation statements)

References 69 publications

(77 reference statements)

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“…When the density of CPMs reached 60%, we transfected four samples with mimic and the other four samples with mimic NC, respectively. The reagents used for transfection were jetPRIME reagent (Polyplus, Illkirch, France) and 50 nM miR-24-3p mimic and mimic NC (GenePharma, Shanghai, China) [ 11 ]. Trizol (TIANGEN, Beijing, China) was added to these eight samples and the total RNA was extracted by standard methods [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…We removed the bones and blood from the leg muscle and then cut it into meat paste. We digested the meat paste with collagenase type I (Gibco, Grand Island, NY, USA) at 37 • C. After digestion, we filtered out the impurities in the mixture and cultured the CPMs in a 10 cm cell culture dish [11]. The culture medium we used was DMEM/F12 medium (Solarbio, Beijing, China) supplemented with 20% fetal bovine serum (Gibco, Grand Island) and 1% penicillin/streptomycin solution (Sangon Biotech, Shanghai, China) [11].…”

Section: Isolation and Culture Of Chicken Primary Myoblasts (Cpms)mentioning

confidence: 99%

“…In our previous study, we collected the leg muscles from two groups of Jinghai yellow chickens (slow- and fast-growing groups) at the age of 300 days for miRNA sequencing to search for new miRNAs related to skeletal development. Through bioinformatics analysis, the outcome revealed that novel_miR_133 and miR-24-3p were two miRNAs that are significantly related to muscle development [ 11 ]. We also demonstrated that miR-24-3p inhibited myoblast proliferation while promoting their differentiation.…”

Section: Introductionmentioning

confidence: 99%

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Exploration of Potential Target Genes of miR-24-3p in Chicken Myoblasts by Transcriptome Sequencing Analysis

Ling,

Wang,

et al. 2023

Genes

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Broiler skeletal muscle growth is significantly influenced by miRNAs. Our earlier research demonstrated that miR-24-3p significantly suppressed the proliferation of chicken myoblasts while promoting their differentiation. The purpose of this study is to investigate miR-24-3p potential target genes in chickens. We collected myoblasts of Jinghai yellow chicken and transfected four samples with mimics of miR-24-3p and another four samples with mimic NC (negative control) for RNA-seq. We obtained 54.34 Gb of raw data in total and 50.79 Gb of clean data remained after filtering. Moreover, 11,635 genes were found to be co-expressed in these two groups. The mimic vs. NC comparison group contained 189 DEGs in total, 119 of which were significantly up-regulated and 70 of which were significantly down-regulated. Important biological process (BP) terminology such as nuclear chromosomal segregation, reproduction, and nuclear division were discovered by GO enrichment analysis for DEGs in the mimic vs. NC comparison group. KEGG pathway analysis showed that focal adhesion, cytokine–cytokine receptor interaction, the TGF-β signaling pathway, and the MAPK signaling pathway were enriched in the top 20. Variation site analysis illustrated the SNP (single nucleotide polymorphisms) and INDEL (insertion–deletion) in the tested samples. By comparing the target genes predicted by miRDB (MicroRNA target prediction database) and TargetScan with the 189 DEGs found by the transcriptome sequencing, we discovered two up-regulated DEGs (NEURL1 and IQSEC3) and two down-regulated DEGs (REEP1 and ST6GAL1). Finally, we carried out qPCR experiments on eight DEGs and discovered that the qPCR results matched the sequencing outcomes. These findings will aid in identifying potential miR-24-3p target genes in chicken skeletal muscle and offer some new directions for upcoming research on broiler breeding.

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Section: Methodsmentioning

confidence: 99%

Section: Isolation and Culture Of Chicken Primary Myoblasts (Cpms)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exploration of Potential Target Genes of miR-24-3p in Chicken Myoblasts by Transcriptome Sequencing Analysis

Ling,

Wang,

et al. 2023

Genes

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“…In mice, miR-122 mediates the TGF-β/Smad signaling pathway through inhibition of TGFBR2 expression, contributing to muscle production and repair after injury ( Ding et al, 2020 ). miR-24-3p was found to have positive regulatory effects on myoblast differentiation in fetal cattle ( Hu et al, 2019 ), mice ( Holstein et al, 2020 ), and chickens ( Wu et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene

Zhou,

Wu,

Ling

et al. 2024

Poultry Science

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miRNA-seq analysis in skeletal muscle of chicken and function exploration of miR-24-3p

Cited by 2 publications

References 69 publications

Exploration of Potential Target Genes of miR-24-3p in Chicken Myoblasts by Transcriptome Sequencing Analysis

Exploration of Potential Target Genes of miR-24-3p in Chicken Myoblasts by Transcriptome Sequencing Analysis

miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene

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