miRNA editing landscape reveals miR-34c regulated spermatogenesis through structure and target change in pig and mouse

Wang, Xiaodan; Zhang, Peng; Li, Leijie; Che, Dongxue; Li, Tongtong; Li, Hao; Li, Qun; Jia, Haiyang; Tao, Shiheng; Hua, Jinlian; Liao, Mingzhi

doi:10.1016/j.bbrc.2018.05.197

Cited by 11 publications

(10 citation statements)

References 25 publications

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“…The well-known myomiRs (miRNA-1, miR-133a/b, and miR-206) were specifically expressed in skeletal muscle. In testis, the top two tissue-specific miRNAs, miR-34c and miR-202-5p, were shown to possess an important influence in spermatogenesis regulation (Dabaja et al, 2015; Wang et al, 2018b). For fat, only one known miRNA (miR-224), which was reported to have an important role in adipogenesis development, was specifically expressed (Peng et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Profiles of mRNAs and miRNAs Reveal Molecular Characteristics of Multiple Organ Physiologies and Development in Pigs

et al. 2019

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The pig (Sus scrofa) is not only an important livestock animal but also widely used as a biomedical model. However, the understanding of the molecular characteristics of organs and of the developmental skeletal muscle of the pig is severely limited. Here, we performed a comprehensive transcriptome profiling of mRNAs and miRNAs across nine tissues and three skeletal muscle developmental stages in the Guizhou miniature pig. The reproductive organs (ovary and testis) had greater transcriptome complexity and activity than other tissues, and the highest transcriptome similarity was between skeletal muscle and heart (R = 0.79). We identified 1,819 mRNAs and 96 miRNAs to be tissue-specific in nine organs. Testis had the largest number of tissue-specific mRNAs (992) and miRNAs (40). Only 15 genes and two miRNAs were specifically expressed in skeletal muscle and fat, respectively. During postnatal skeletal muscle development, the mRNAs associated with focal adhesion, Notch signaling, protein digestion, and absorption pathways were up-regulated from D0 to D30 and then down-regulated from D30 and D240, while genes with opposing expression patterns were significantly enriched in the oxidative phosphorylation and proteasome pathways. The miRNAs mainly regulated genes associated with insulin, Wnt, fatty acid biosynthesis, Notch, MAPK, TGF-beta, insulin secretion, ECM–receptor interaction, focal adhesion, and calcium signaling pathways. We also identified 37 new miRNA–mRNA interaction pairs involved in skeletal muscle development. Overall, our data not only provide a rich resource for understanding pig organ physiology and development but also aid the study of the molecular functions of mRNA and miRNA in mammals.

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Section: Resultsmentioning

confidence: 99%

Comprehensive Profiles of mRNAs and miRNAs Reveal Molecular Characteristics of Multiple Organ Physiologies and Development in Pigs

et al. 2019

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“…Thus, during this period, the energy intake in dairy cows is not able to meet the demand for milk production (especially in high-yielding cows). AMPK signaling in the mammary gland is highly active during this period, which results in a 15-20% decrease in milk production in sows and cows (Eastham et al, 1988;Wang et al, 2018). In vitro experiments also demonstrated that AMPK is significantly activated in mammary epithelial cells during energy deprivation (cells cultured without glucose or amino acids) (Zhang M. et al, 2018).…”

Section: Activation Of Ampk In the Mammary Glandmentioning

confidence: 99%

Current Evidences and Future Perspectives for AMPK in the Regulation of Milk Production and Mammary Gland Biology

Tian

Heng

et al. 2020

Front. Cell Dev. Biol.

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Activated protein kinase (AMP)-activated protein kinase (AMPK) senses the cellular energy status and coordinates catabolic and anabolic processes. Extensive studies have proposed that AMPK regulates energy homeostasis, cell growth, autophagy, mitochondrial biology and inflammation. The biological functions of AMPK vary in different tissues or organs. As a unique organ that produces milk, the mammary gland has recently attracted substantial research attention. This review discusses how AMPK in the mammary gland is activated by energy deprivation and heat stress via the activation of canonical and non-canonical pathways. In addition, the important downstream targets of AMPK and their functions in the mammary gland, especially during milk synthesis, are summarized in the review.

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“…Because of their important regulatory roles, they can be considered as biomarkers for screening and identifying male infertility (9). MiR-34c is one of the significant miRNAs contributing to mammalian spermatogenesis (10, 11) and is highly expressed in adult male testicles where it controls the proliferation and differentiation of spermatogonia cells (12) and is involved in the four stages of spermatogenesis (13). Altered expression of miR-34c has been shown in various infertility disorders such as oligoasthenozoospermia, asthenozoospermia, and azoospermia (10,14).…”

Section: Introductionmentioning

confidence: 99%

Cholestasis Alters miR-34c-5p Expression in the Testes of Male Wistar Rats

2021

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Background: Cholestasis is a pathophysiological condition, significantly reducing spermatozoa production. MiR-34c is highly expressed in adult male testicles and controls different stages of spermatogenesis. Objectives: Here, we aimed to investigate miR-34c expression in the testes of rat models of cholestasis. The expressions of THY-1, FGF-2, and CASP-3 genes, that are targeted by mirR-34c were also investigated. Methods: Cholestasis was induced in six adult rats via bile duct ligation. Four weeks after cholestasis induction, sera and testicular tissues were collected for further examinations. The levels of liver enzymes were measured using the ELISA. The structure of the testes was evaluated by histological examination. Total RNA was extracted from testes using a special kit and converted to cDNA. The expressions of miR-34c-5p, THY-1, FGF-2, and CASP-3 genes were determined by Real-Time PCR. Results: The serum levels of ALP, AST, and ALT were significantly elevated in the rat models of cholestasis (P < 0.001). Real-Time PCR revealed that the expressions of miR-34c-5p, THY-1, and FGF-2 genes decreased while CASP-3 gene was upregulated in the testes of cholestatic animals (all differences were significant at P < 0.05). Conclusions: Our study indicated that cholestasis was associated with reduced expression of miR-34c and altered expression of its target genes in the testis. Our results highlight the potential effects of cholestasis, a hepatobiliary disease, on testicular tissue function and male fertility.

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miRNA editing landscape reveals miR-34c regulated spermatogenesis through structure and target change in pig and mouse

Cited by 11 publications

References 25 publications

Comprehensive Profiles of mRNAs and miRNAs Reveal Molecular Characteristics of Multiple Organ Physiologies and Development in Pigs

Comprehensive Profiles of mRNAs and miRNAs Reveal Molecular Characteristics of Multiple Organ Physiologies and Development in Pigs

Current Evidences and Future Perspectives for AMPK in the Regulation of Milk Production and Mammary Gland Biology

Cholestasis Alters miR-34c-5p Expression in the Testes of Male Wistar Rats

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