miRNA-346 promotes proliferation, migration and invasion in liver cancer

Yu, Qiang; Xia, Yang; Duan, Weidong; Li, Chonghui; Luo, Ying; Lu, Shichun

doi:10.3892/ol.2017.6561

Cited by 36 publications

(17 citation statements)

References 33 publications

(34 reference statements)

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“…Similarly, the downregulation of miR-346 led to an activation of the proliferation, migration and invasion of gestational choriocarcinoma cells, and this phenomenon was also be reversed by siLRP6 treatment. miR-346 is known to have a tumor-promoter function in a variety of tumor cells, including in liver cancer (27), nasopharyngeal cancer (28), and breast cancer (29). However, contrary to previous findings, the present study revealed that downregulated miR-346 promoted the proliferation, migration and invasion of trophoblast cells.…”

Section: Discussioncontrasting

confidence: 99%

LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR‑346

Zhang

et al. 2020

Int J Mol Med

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Low-density lipoprotein receptor-related protein 6 (LRP6) promotes metastasis in numerous types of cancer; however, its role in trophoblast cells has been less frequently reported. In the present study, the effects of up-and downregulation of LRP6 on trophoblast cells were investigated accordingly. The study aimed to develop a therapeutic target for gestational choriocarcinoma. The expression levels of LRP6 in pre-eclampsia (PE) tissues, trophoblast cell lines and gestational choriocarcinoma cells were determined using reverse transcription-quantitative polymerase chain reaction (RT-qPcR) assay. double-luciferase reporter analysis was conducted to detect the regulatory gene of LRP6. Furthermore, the proliferative, migratory and invasive abilities of trophoblasts and gestational choriocarcinoma cells were determined by ccK-8, wound healing, and Transwell assays, respectively. The expression levels of the genes and proteins of interest [matrix metalloproteinase (MMP)-2, MMP-9, tissue inhibitor of metalloproteinase-1 (TIMP-1), and TIMP-2] associated with tumor cell invasion were measured by performing RT-qPcR and western blotting, respectively. The National center for Biotechnology Information database revealed that LRP6 was relatively highly expressed in placental tissues, but was poorly expressed in PE tissues and trophoblast cell lines. The upregulation of LRP6 not only increased the activity, migration and invasion of trophoblast cells, but it also promoted the expression of MMP-2 and MMP-9, whereas it inhibited the expression levels of TIMP-1 and TIMP-2. Such results followed the opposite trend to those of downregulation of LRP6 in gestational choriocarcinoma cells. Moreover, LRP6 was predicted to be the target gene for microRNA (miR)-346, which was highly expressed in PE tissues and trophoblast cell lines. The present study also revealed that LRP6 could reverse the effects of miR-346 on the proliferation, migration and invasion of trophoblast cells. Therefore, considered collectively, the results of the present study have demonstrated that LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR-346, and that LRP6 may serve as a potential target in cancer treatment.

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Section: Discussioncontrasting

confidence: 99%

LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR‑346

Zhang

et al. 2020

Int J Mol Med

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“…Its role is important in the case of cancer biology; several studies showed its oncogenic nature in numerous cancers, which was responsible for promotion of the more aggressive phenotype of disease. It has been shown that overexpression of hsa-miR-346 promoted cell proliferation, colony formation, migration, invasion in nasopharyngeal, colorectal, and liver cancer [32][33][34][35]. Moreover, it was responsible for reduced apoptosis and resistance to docetaxel of breast cancer cells [36] Most of the downregulated miRNAs detected in our study in the metastatic patient subgroup belong to the miRNA-506-513 cluster, which is consistent with previous TCGA-based studies [5,18].…”

Section: Discussionsupporting

confidence: 89%

The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression

Wróblewska

Lach

Ustaszewski

et al. 2020

Genes

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Uveal melanoma (UM) is the most common primary tumor of the eye diagnosed in adults, associated with a high risk of metastasis and thereby, poor prognosis. Among known risk factors for the development of metastatic disease is the loss of BAP1 expression and chromosome 3 monosomy in the primary tumor. However, the expression levels of specific micro RNAs (miRNA) in tumor tissue may also serve as a valuable marker for determining the risk of metastatic disease in patients with primary uveal melanoma. In our study, we analyzed the miRNA expression data of cases selected from The Cancer Genome Atlas study on uveal melanoma, and determined a panel of 15 miRNAs differentially expressed between patients with primary and metastatic disease. Next, 6 miRNAs were validated on a group of 46 tumor samples from primary and metastatic patients. We have shown, that expression of hsa-miR-592, hsa-miR-346, and hsa-miR-1247 was significantly increased, while hsa-miR-506 and hsa-miR-513c were decreased in the tumors of patients with metastatic disease. Hsa-miR-196b expression did not differ between the two subgroups, however, we showed significant correlation with BAP1 expression. Moreover, hsa-miR-592 also showed correlation with monosomy 3 tumors. Gene ontology analysis revealed involvement of those miRNAs with cellular processes mediating the metastatic process. Our results showed that miRNAs play an important role in the deregulation of several oncogenic pathways in UM and can, thereby, promote metastatic spread to distant organs. Moreover, differentially expressed miRNAs may be used as an interesting biomarker for the assessment of metastatic risk in uveal melanoma patients.

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“…On the contrary, miR-21 can promote the proliferation of liver cancer, whose mechanism may be related to the direct targeted inhibition of miR-21 on MAP kinase-kinase 3 (MAP2K3) (18). Moreover, miR-346 also serves as a cancer-promoting gene, which can facilitate the proliferation, invasion and metastasis of liver cancer cells through targeted inhibition on F-Box and leucine-rich repeat protein (FBXL2) (19). In the present study, it was found for the first time, to the best of our knowledge, that the miR-101 expression level in liver cancer tissues was significantly lower than that in para-carcinoma tissues, indicating that miR-101 may play a role as a cancer suppressor gene.…”

Section: Discussionmentioning

confidence: 99%

Influence of miR‑101 on proliferation of liver cancer cells through the MAPK/ERK signaling pathway

et al. 2019

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miRNA-346 promotes proliferation, migration and invasion in liver cancer

Cited by 36 publications

References 33 publications

LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR‑346

LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR‑346

The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression

Influence of miR‑101 on proliferation of liver cancer cells through the MAPK/ERK signaling pathway

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