miRNA‐216 knockdown has effects to suppress osteosarcoma via stimulating PTEN

Ping, Jiang; Yang, Xin; Li, Yuanli; Chen, Juan

doi:10.1002/fsn3.1587

Cited by 7 publications

(7 citation statements)

References 30 publications

(31 reference statements)

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“…Numerous recent studies have demonstrated the aberrant miRNA expressions in osteosarcoma, which contribute to various aspects of osteosarcoma, including proliferation, differentiation, migration, and apoptosis (Jiang et al, 2020; Liang et al, 2013; Liu et al, 2020; Luo et al, 2020). The miR‐181a is an important miRNA with oncogenic function in osteosarcoma.…”

Section: Discussionmentioning

confidence: 99%

The expression of miR‐181b, CYLD, CBX‐7, BCL2, and p53 in osteosarcoma patients and correlation with clinicopathological factors

et al. 2022

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Osteosarcoma is a common human malignancy with a high mortality rate worldwide. Recent studies have been focused on understanding the involvement of microRNA (miRNAs) in the pathogenesis of osteosarcoma. Therefore, the present study aimed to measure the expression levels of miR‐181a, cylindromatosis (CYLD), chromo box homolog 7 (CBX7), B‐cell lymphoma 2 (BCL2), and tumor protein p53 in tumor tissue and adjacent normal tissues in patients with osteosarcoma and its relationship with clinicopathological factors. The expression levels of miR‐181a, CYLD, CBX7, BCL2, and p53 were measured in 60 patients with osteosarcoma using quantitative real‐time polymerase chain reaction. Finally, we compared the relationship between these gene levels and clinicopathological factors in tumor and healthy tissues. Our results showed that the expression levels of miR‐181a, BCL2, and p53 were significantly higher in osteosarcoma tissue in comparison with normal tissues (p < .05). On the contrary, CYLD and CBX7 were downregulated in osteosarcoma tumor tissues compared to adjacent healthy tissues (p < .05). In addition, the expression levels of miR‐181a in tumor tissues were strongly correlated with patients' age, tumor size, clinical stage, cancer grade, and lymph node metastasis (p < .05). Our findings highlight new insights into understanding the role of miR‐181a in the pathogenesis of osteosarcoma. However, further studies are needed to elucidate miRNA as therapeutic targets for osteosarcoma.

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Section: Discussionmentioning

confidence: 99%

The expression of miR‐181b, CYLD, CBX‐7, BCL2, and p53 in osteosarcoma patients and correlation with clinicopathological factors

et al. 2022

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“…miRNA-216 knockdown promotes apoptosis through PTEN/PI3K/AKT and related downstream genes P53 and MMP-2/9, thus exerting antitumor effects. 104 High expression of miRNA-1226-3p was associated with lower overall survival. It can inhibit the proliferation, migration, and invasion of osteosarcoma cells by targeting TRIAP3 and promote the apoptosis of osteosarcoma cells.…”

Section: The Role and Mechanism Of Mirnas In The Development Of Osteo...mentioning

confidence: 98%

“… 101 miRNA-21, miRNA-216, miRNA-155, and miRNA-524 can enhance the proliferation of osteosarcoma cells by targeting PTEN to activate the PI3K/Akt signaling pathway. 102 – 105 The PI3K/AKT signaling pathway is unregulated in most localized diseases and in 100% of advanced diseases, implying that alterations in this pathway may be a prerequisite for inhibiting osteosarcoma progression. 106…”

Section: The Role and Mechanism Of Mirnas In The Development Of Osteo...mentioning

confidence: 99%

“…Studies on ferroptosis in osteosarcoma may provide some effective ways to address these problems. 104 , 159 , 160 It plays a vital role in cancer and many degenerative diseases. 72 , 161 Ferroptosis was initially described as a non-apoptotic form of cell death characterized by impaired cellular uptake of cysteine, glutathione (GSH) depletion, and iron-dependent lipid peroxidation.…”

Section: The Role and Mechanism Of Mirnas In The Development Of Osteo...mentioning

confidence: 99%

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Advances in the Biological Functions and Mechanisms of miRNAs in the Development of Osteosarcoma

Dong

Liao

et al. 2022

Technol Cancer Res Treat

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Osteosarcoma is one of the most common primary malignant bone tumors, mainly occurring in children and adolescents, and is characterized by high morbidity and poor prognosis. MicroRNAs, a class of noncoding RNAs consisting of 19 to 25 nucleotides, are involved in cell proliferation, invasion, metastasis, and apoptosis to regulate the development and progression of osteosarcoma. Studies have found that microRNAs are closely related to the diagnosis, treatment, and prognosis of osteosarcoma patients and have an important role in improving drug resistance in osteosarcoma. This paper reviews the role of microRNAs in the pathogenesis of osteosarcoma and their clinical value, aiming to provide a new research direction for diagnosing and treating osteosarcoma and achieving a better prognosis.

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“…For example, knockdown of miR-216 suppresses the progression of osteosarcoma by targeting PTEN. 19 MiR-183-5p attenuates cerebral ischemia injury by negatively regulating PTEN. 9 In this study, we found that miR-195-5p was significantly downregulated during cerebral I/R injury.…”

Section: Introductionmentioning

confidence: 99%

MiR-195-5p Ameliorates Cerebral Ischemia-Reperfusion Injury by Regulating the PTEN-AKT Signaling Pathway

Ren¹,

Wang²,

Guo³

2021

NDT

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Background MiR-195-5p has been shown to play crucial roles in tumor inhibition, but its biological functions in cerebral ischemia-reperfusion (I/R) injury are unclear. Methods To mimic cerebral I/R injury, mice were induced by transient middle cerebral artery occlusion (MCAO). Human brain microvascular endothelial cells (HBMVECs) were treated with oxygen-glucose deprivation (OGD) to mimic I/R injury in vitro. The expression of miR-195-5p and PTEN was detected by qRT-PCR or Western blot. Cell viability was evaluated by CCK-8 assay. Cell apoptosis was detected by flow cytometer. Cell death was detected using specific lactate dehydrogenase (LDH) cytotoxicity kit. Infarct volume in mice brains was evaluated by TTC staining. Histopathological analysis was performed by HE staining and TUNEL staining. The interaction between miR-195-5p and PTEN was determined by TargetScan and luciferase reporter assay. Results MiR-195-5p was significantly downregulated and PTEN was upregulated during cerebral I/R injury both in vitro and in vivo. Overexpression of miR-195-5p efficiently enhanced cell viability, while reduced LDH release and apoptotic rate of OGD-treated HBMVECs in vitro. MiR-195-5p could negatively regulate the expression of PTEN by directly binding to its 3′-UTR. Overexpression of PTEN obviously attenuated the protective effect of miR-195-5p mimics on cell viability, LDH release and apoptosis in OGD-treated HBMVECs. Meanwhile, overexpression of miR-195-5p increased the expression levels of p-AKT in OGD-treated HBMVECs, while this effect was reversed by overexpression of PTEN. Moreover, overexpression of miR-195-5p efficiently ameliorated brain injury of mice after MCAO treatment in vivo. Conclusion Overexpression of miR-195-5p ameliorated cerebral I/R injury by regulating the PTEN-AKT signaling pathway, providing a potential therapeutic target for cerebral I/R injury.

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miRNA‐216 knockdown has effects to suppress osteosarcoma via stimulating PTEN

Cited by 7 publications

References 30 publications

The expression of miR‐181b, CYLD, CBX‐7, BCL2, and p53 in osteosarcoma patients and correlation with clinicopathological factors

The expression of miR‐181b, CYLD, CBX‐7, BCL2, and p53 in osteosarcoma patients and correlation with clinicopathological factors

Advances in the Biological Functions and Mechanisms of miRNAs in the Development of Osteosarcoma

MiR-195-5p Ameliorates Cerebral Ischemia-Reperfusion Injury by Regulating the PTEN-AKT Signaling Pathway

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