miRNA-10a-5p Targeting the BCL6 Gene Regulates Proliferation, Differentiation and Apoptosis of Chicken Myoblasts

Zhang, Genxi; Zhang, Xinchao; Zhou, Kaizhi; Ling, Xuanze; Zhang, Jin; Wu, Pengfei; Tao, Zhang; Xie, Kaizhou; Dai, Guojun

doi:10.3390/ijms23179545

Cited by 11 publications

(8 citation statements)

References 38 publications

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“…First, the supernatant with single cells was inoculated into a 10-cm plate and placed the plate in the incubator. After 40 min, the supernatant was transferred to a new 10-cm dish, repeating the above steps 3 times to remove fibroblasts ( Li et al, 2018 ; Zhang et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene

Zhou,

Wu,

Ling

et al. 2024

Poultry Science

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Section: Methodsmentioning

confidence: 99%

miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene

Zhou,

Wu,

Ling

et al. 2024

Poultry Science

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“…Finally, the active complex interacts with the 3′-UTR of target mRNA through complementary pairing of base pairings, causing translational repression or RNA degradation (Figure 1A). 83−85 Through this process, miRNAs were involved in a series of cellular processes, including apoptosis, 4−6 oxidative stress, 7 cell proliferation, 8,9 inflammation, 10,11 hematopoiesis, 4,12 and tumorigenesis. 13,14 Recent studies revealed that miRNAs not only have an intracellular function but also stably exist in biological fluids such as saliva, cerebrospinal fluid, plasma, serum, gingival fluid, and urine.…”

Section: Micrornasmentioning

confidence: 99%

“…These functional miRNAs form the RISC via binding to AGO. Finally, the active complex interacts with the 3′-UTR of target mRNA through complementary pairing of base pairings, causing translational repression or RNA degradation (Figure A). − Through this process, miRNAs were involved in a series of cellular processes, including apoptosis, − oxidative stress, cell proliferation, , inflammation, , hematopoiesis, , and tumorigenesis. , …”

Section: Micrornasmentioning

confidence: 99%

“…A particular miRNA can target various mRNAs to induce translational repression or RNA degradation, and one mRNA can be regulated by multiple different miRNAs . By modulating gene expression at the post-translational level, miRNAs play a critical role in a series of cellular processes, including apoptosis, − oxidative stress, cell proliferation, , inflammation, , hematopoiesis, , and tumorigenesis. , Importantly, miRNAs not only have an intracellular function but also stably exist in biological fluids such as saliva, cerebrospinal fluid, plasma, serum, gingival fluid, and urine. − Therefore, circulating miRNAs are considered biomarkers of disease and can regulate gene expression in distal cells or organs. ,, …”

Section: Introductionmentioning

confidence: 99%

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The Role of microRNAs in Arsenic-Induced Human Diseases: A Review

Liu,

Lei

2023

J. Agric. Food Chem.

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MicroRNAs (miRNAs) are noncoding RNAs with 20–22 nucleotides, which are encoded by endogenous genes and are capable of targeting the majority of human mRNAs. Arsenic is regarded as a human carcinogen, which can lead to many adverse health effects including diabetes, skin lesions, kidney disease, neurological impairment, male reproductive injury, and cardiovascular disease (CVD) such as cardiac arrhythmias, ischemic heart failure, and endothelial dysfunction. miRNAs can act as tumor suppressors and oncogenes via directly targeting oncogenes or tumor suppressors. Recently, miRNA dysregulation was considered to be an important mechanism of arsenic-induced human diseases and a potential biomarker to predict the diseases caused by arsenic exposure. Endogenic miRNAs such as miR-21, the miR-200 family, miR-155, and the let-7 family are involved in arsenic-induced human disease by inducing translational repression or RNA degradation and influencing multiple pathways, including mTOR/Arg 1, HIF-1α/VEGF, AKT, c-Myc, MAPK, Wnt, and PI3K pathways. Additionally, exogenous miRNAs derived from plants, such as miR-34a, miR-159, miR-2911, miR-159a, miR-156c, miR-168, etc., among others, can be transported from blood to specific tissue/organ systems in vivo. These exogenous miRNAs might be critical players in the treatment of human diseases by regulating host gene expression. This review summarizes the regulatory mechanisms of miRNAs in arsenic-induced human diseases, including cancers, CVD, and other human diseases. These special miRNAs could serve as potential biomarkers in the management and treatment of human diseases linked to arsenic exposure. Finally, the protective action of exogenous miRNAs, including antitumor, anti-inflammatory, anti-CVD, antioxidant stress, and antivirus are described.

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“…Many studies confirmed that some regulatory genes have an essential impact in the incidence and development of IDD, such as microRNA (miRNA). The miRNA are single-stranded noncoding small RNA with 18 to 24 bp nucleotide sequences, which participates in regulating the cell proliferation and apoptosis [21][22][23][24][25]. And miRNA can negatively regulate the posttranscriptional gene expression in different species by either inhibiting mRNA translation or promoting mRNA degradation [25,26].…”

Section: Introductionmentioning

confidence: 99%

miR-328-5p Induces Human Intervertebral Disc Degeneration by Targeting WWP2

Yan

Zhang

et al. 2022

Oxidative Medicine and Cellular Longevity

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Intervertebral disc degeneration (IDD) development is regulated by miRNA, including inflammatory reactions, cell apoptosis, and degradation of extracellular matrix. Nucleus pulposus cells apoptosis has a absolute influence in the development of IDD. This experiment explores the mechanism of miR-328-5p regulating IDD. Through the analysis of miRNA and mRNA microarray database, we screened the target genes miR-328-5p and WWP2. We verified the expression of miR-328-5p, WWP2, and related apoptotic genes in normal and degenerative nucleus pulposus tissues by qRT-PCR. The expressions of WWP2, Bcl-2, and Bax were detected by qRT-PCR and western blot after transfection to nucleus pulposus cell. The nucleus pulposus cell proliferation and apoptosis after transfection were confirmed by CCK8 and flow cytometry. Luciferase reporter assay and bioinformatics analyzed the targeting relationship between miR-328-5p and WWP2. Firstly, the qRT-PCR experiments confirmed the significant increase of miR-328-5p expression, while significant reduction of WWP2 in a degenerative tissues compared to the normal tissues. Surprisingly, miR-328-5p expression was positively, while that of WWP2 negatively correlated with the degeneration grade of IDD. And we also identified the high expression of Bax and Caspase3, while low expression of Bcl-2 in a degenerative tissues. After miR-328-5p mimic transfected into nucleus pulposus cell, qRT-PCR and western blot confirmed that WWP2 and Bcl-2 expressions were downregulated, while Bax and Caspase3 expressions were upregulated, and the same results were obtained by knocking down WWP2. CCK8 and flow cytometry confirmed that miR-328-5p inhibited the proliferation and induced apoptosis of nucleus pulposus cells. WWP2 is a target gene of miR-328-5p by bioinformatics and luciferase reporter assay. In summary, miR-328-5p targets WWP2 to regulate nucleus pulposus cells apoptosis and then participates in the development of IDD. Furthermore, this study may provide new references and ideas for IDD treatment.

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miRNA-10a-5p Targeting the BCL6 Gene Regulates Proliferation, Differentiation and Apoptosis of Chicken Myoblasts

Cited by 11 publications

References 38 publications

miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene

miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene

The Role of microRNAs in Arsenic-Induced Human Diseases: A Review

miR-328-5p Induces Human Intervertebral Disc Degeneration by Targeting WWP2

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