MiR200c targets IRS1 and suppresses prostate cancer cell growth

Su, Wenjing; Xu, Miao; Chen, Xueqin; Nie, Ling; Chen, Ni; Gong, Jing; Zhang, Mengni; Su, Zhengzheng; Huang, Lei; Zhou, Qiao

doi:10.1002/pros.22968

Cited by 18 publications

(13 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we show that HIF-1α mRNA is a functionally relevant target of miR-199a-5p, the downregulation of which contributes to the oncogenic high level of HIF-1α in Pca [ 11 , 16 – 18 ], as well as tumor progression and unfavorable patient survival.…”

Section: Discussionmentioning

confidence: 98%

“…For example, underexpression of let-7c, miR-100, miR-145 and miR-218 in PCa was found to be associated with metastasis, and overexpression of miR-182 and miR-96 indicates a higher risk of progression [ 14 , 15 ]. In our previous reports, we demonstrated that downregulation of miR-145, miR-200c, and miR-199b is significantly associated with PCa progression; in contrast, we found hypoxia-inducible factor (HIF-1α), which may be suppressed by miR-199b, was overexpressed in PCa and inhibited apoptosis via BCL2-xL transactivation [ 11 , 16 – 18 ]. Expression of miR-199a-5p has been reported to be downregulated in certain tumors, including malignant testicular tumors, colorectal cancer and hepatocellular carcinoma, but upregulated in others, such as melanoma, gastric cancer and pancreatic adenocarcinoma [ 19 – 24 ], indicating tumor-type-specific regulatory mechanisms.…”

Section: Introductionmentioning

confidence: 93%

See 1 more Smart Citation

Downregulation of miR-199a-5p promotes prostate adeno-carcinoma progression through loss of its inhibition of HIF-1α

Zhong

Huang

et al. 2017

Oncotarget

Self Cite

View full text Add to dashboard Cite

Hypoxia-inducible factor-1 alpha (HIF-1α) plays key roles in cell survival under both hypoxia and normoxia conditions. Regulation of HIF-1α is complex and involves numerous molecules and pathways, including post-transcriptional regulation by microRNAs (miRNAs). Although upregulation of HIF-1α has been shown to promote prostate adenocarcinoma (PCa) progression, the mechanism by which miRNAs modulate HIF-1α in prostate cancer has not been clarified. Here, we show that miR-199a-5p is underexpressed in prostate adenocarcinoma. Artificial overexpression of miR-199a-5p decreased cell proliferation, motility, and tumor angiogenesis and increased apoptosis in PCa cell liness PC-3 and DU145 by directly targeting the 3’-untranslated region (UTR) of HIF-1α mRNA, which reduced HIF-1α levels as well as downstream genes transactivated by HIF-1α (such as VEGF, CXCR4, BNIP3 and BCL-xL). Abnormalities of miR-199a-HIF regulation may contribute significantly to PCa pathogenesis and progression.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 93%

Downregulation of miR-199a-5p promotes prostate adeno-carcinoma progression through loss of its inhibition of HIF-1α

Zhong

Huang

et al. 2017

Oncotarget

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, several other genes present in the OncoScore lists, such as KLLN, LZTS1, BNIP3L, ING4 as well as MIR200C microRNA were recently implicated in the development of prostate cancer1415161718. Taken globally these data indicate that OncoScore is a useful prioritization tool for the annotation of copy-number abnormalities in human cancer.…”

Section: Resultsmentioning

confidence: 75%

OncoScore: a novel, Internet-based tool to assess the oncogenic potential of genes

Piazza

Ramazzotti

Spinelli

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Of them 74 had an OncoScore > 0 and 47 an OncoScore ≥ 21. A total of 8 cancer genes with an OncoScore above the 90 th percentile was identified (TNFRSF10B, [14][15][16][17][18] . Taken globally these data indicate that OncoScore is a useful prioritization tool for the annotation of copy-number abnormalities in human cancer.…”

Section: Oncoscore Analysis Of Large Structural Variationsmentioning

confidence: 99%

OncoScore: a novel, Internet-based tool to assess the oncogenic potential of genes

Ramazzotti

Spinelli

Pirola³

et al. 2017

Preprint

View full text Add to dashboard Cite

The complicated, evolving landscape of cancer mutations poses a formidable challenge to identify cancer genes among the large lists of mutations typically generated in NGS experiments. The ability to prioritize these variants is therefore of paramount importance. To address this issue we developed OncoScore, a text-mining tool that ranks genes according to their association with cancer, based on available biomedical literature. Receiver operating characteristic curve and the area under the curve (AUC) metrics on manually curated datasets confirmed the excellent discriminating capability of OncoScore (OncoScore cut-off threshold = 21.09; AUC = 90.3%, 95% CI: 88.1-92.5%), indicating that OncoScore provides useful results in cases where an efficient prioritization of cancer-associated genes is needed.The huge amount of data emerging from NGS projects is bringing a revolution in molecular medicine, leading to the discovery of a large number of new somatic alterations that are associated with the onset and/or progression of cancer. However, researchers are facing a formidable challenge in prioritizing cancer genes among the variants generated by NGS experiments. Despite the development of a significant number of tools devoted to cancer driver prediction, limited effort has been dedicated to tools able to generate a gene-centered Oncogenic Score based on the evidence already available in the scientific literature. To overcome these limitations, we propose here OncoScore, a bioinformatics text-mining tool capable of automatically scanning the biomedical literature by means of dynamically updatable web queries and measuring gene-specific cancer association in terms of gene citations. The output of this analysis is a score representing the strength of the association of any gene symbol to cancer, based on the literature available at the time of the analysis. OncoScore is distributed as a R Bioconductor package (https://bioconductor.org/packages/release/bioc/html/OncoScore.html) in order to allow full customization of the algorithm and easy integration in existing NGS pipelines, and as a web tool for easy access by researchers with limited or no experience in bioinformatics (http://www.galseq.com/oncoscore.html). ResultsWe analyzed the performance of OncoScore on the Cancer Genes Census (CGC; Supplementary Table 1), a collection of regularly updated and manually annotated genes accepted as causally implicated in oncogenesis 1 . To assess the ability of OncoScore to discriminate between cancer and non-cancer genes we generated the OncoScore estimation for the whole CGC dataset and for a manually curated list of genes not associated with cancer (named nCan; Supplementary Table 2; see Methods section for further details). Genes with a total citation count < 10 publications were filtered out, therefore from a total of 507 CGC and 302 nCan, 472 (93.1%) and 266 (88.1%) genes were further processed.OncoScore performance. The distribution of OncoScore values differed significantly between the two groups (mean: 48.8 and 14.8 for C...

show abstract

MiR200c targets IRS1 and suppresses prostate cancer cell growth

Abstract: Our results suggest that miR200c plays crucial roles in prostate cancer by post-transcriptional regulation of IRS1. The mir200c/IRS1 pathway may be a potential therapeutic target to prevent prostate cancer cell growth.

Cited by 18 publications

References 37 publications

Downregulation of miR-199a-5p promotes prostate adeno-carcinoma progression through loss of its inhibition of HIF-1α

Downregulation of miR-199a-5p promotes prostate adeno-carcinoma progression through loss of its inhibition of HIF-1α

OncoScore: a novel, Internet-based tool to assess the oncogenic potential of genes

OncoScore: a novel, Internet-based tool to assess the oncogenic potential of genes

Contact Info

Product

Resources

About