miR‐874‐3p inhibits cell migration through targeting RGS4 in osteosarcoma

Liu, Weigang; Zhuo, Lang; Lü, Yun; Wang, Lin; Ji, Yanxia; Guo, Qi

doi:10.1002/jgm.3213

Cited by 21 publications

(21 citation statements)

References 24 publications

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“…MicroRNA‐874‐3p (miR‐874‐3p) was affirmed as a tumour inhibitor in osteosarcoma, epithelial ovarian cancer and oesophageal squamous cell carcinoma by targeting different genes 21‐23 . The development of CRC and chemoresistance was also retarded by miR‐874‐3p 24,25 .…”

Section: Introductionmentioning

confidence: 99%

Circ_0007142 downregulates miR‐874‐3p‐mediated GDPD5 on colorectal cancer cells

Wang

Hongshu

Wei

2021

Eur J Clin Investigation

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Background Ferroptosis is an iron‐dependent and oxidative cell death form. Recent studies suggested that circular RNAs (circRNAs) regulated ferroptosis in tumour cells. Circ_0007142 was identified as a carcinogenic molecule in colorectal cancer (CRC), but its function on ferroptosis in CRC remains unknown. Methods Circ_0007142, microRNA‐874‐3p (miR‐874‐3p) and glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5) levels were assayed using the quantitative real‐time polymerase chain reaction (qRT‐PCR). Cell survival and proliferation were measured by Cell Counting Kit‐8 (CCK‐8) assay. Protein detection was performed by Western blot. Cell apoptosis was analysed by flow cytometry. Ferroptosis was assessed by iron accumulation and oxidative stress. Target binding was evaluated by dual‐luciferase reporter assay. In vivo research was conducted by tumour xenograft in mice. Results Circ_0007142 was overexpressed in CRC. After expression inhibition of circ_0007142, proliferation was reduced, while apoptosis and ferroptosis were facilitated in CRC cells. Mechanically, circ_0007142 was found as a miR‐874‐3p sponge and miR‐874‐3p inhibitor eliminated the regulation of si‐circ_0007142 in CRC cells. MiR‐874‐3p targeted GDPD5 and upregulation of GDPD5 reversed the miR‐874‐3p‐triggered tumour inhibition and ferroptosis promotion in CRC cells. Moreover, GDPD5 was regulated by the circ_0007142/miR‐874‐3p axis. Circ_0007142 also affected CRC tumorigenesis in vivo through the regulation of miR‐874‐3p and GDPD5. Conclusion All these findings proved that circ_0007142/miR‐874‐3p/GDPD5 axis regulated tumorigenesis and ferroptosis of CRC cells. Circ_0007142 might be an available marker for ferroptosis in CRC therapy.

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Section: Introductionmentioning

confidence: 99%

Circ_0007142 downregulates miR‐874‐3p‐mediated GDPD5 on colorectal cancer cells

Wang

Hongshu

Wei

2021

Eur J Clin Investigation

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“…RGS4 was indicated to be a target of miR‐874‐3p and overexpression of RGS4 reversed the inhibitory effect of miR‐874‐3p with respect to the proliferation and migration of osteosarcoma cells 34 . RGS4 expression was up‐regulated in colorectal cancer, 35 and up‐regulated RGS4 mRNA was also associated with aggravated progression of thyroid cancer metastasis, 36 glioma, 37,38 ovarian cancer ascites 39 and triple negative breast cell carcinoma 40 .…”

Section: Discussionmentioning

confidence: 98%

Inhibition of lncRNA DCST1‐AS1 suppresses proliferation, migration and invasion of cervical cancer cells by increasing miR‐874‐3p expression

Zhang¹,

Hu²,

Zou³

et al. 2020

The Journal of Gene Medicine

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BackgroundCervical cancer seriously threatens both the health and life of women. We aimed to investigate whether RNA interference of long non‐coding RNA (lncRNA) DCST1‐AS1 could promote miR‐874‐3p expression to affect the proliferation, migration and invasion of cervical cancer cells.MethodsDCST1‐AS1 expression levels in cervical cancer cells and transfection effects were detected by quantitative reverse transcriptase‐polymerase chain reaction analysis. Proliferation, invasion and migration of cells were separately shown by cell‐counting kit‐8, wound healing and transwell assays, and relative protein expression was determined by western blot analysis. Dual‐luciferase reporter and RNA immunoprecipitation assays verified the interaction of DCST1‐AS1 and miR‐874‐3p.ResultsDCST1‐AS1 expression was increased in cervical cancer tissues and cells. The DCST1‐AS1 expression in Hela and SiHa cells was the highest, and so the cells were selected for the next experiment. Inhibition of DCST1‐AS1 suppressed the proliferation, invasion and migration of cervical cancer cells and decreased the expression of KI67, proliferating cell nuclear antigen, matrix metalloproteinase (MMP)‐2 and MMP‐9. miR‐874‐3p expression was increased when cells were transfected with miR‐874‐3p mimic or shRNA‐DCST1‐AS1‐1, and DCST1‐AS1 expression was down‐regulated when cells were transfected with miR‐874‐3p mimic. DCST1‐AS1 can directly target miR‐874‐3p. Furthermore, inhibition of miR‐874‐3p could effectively alleviate the effect of inhibition of DCST1‐AS1 with respect to the proliferation, invasion and migration of cervical cancer cells.ConclusionsInhibition of DCST1‐AS1 suppressed the proliferation, migration and invasion of cervical cancer cells by increasing miR‐874‐3p expression, which could be alleviated by the inhibition of miR‐874‐3p.

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“…[ 2 ] The abnormal expression of miRNAs has recently been associated with several diseases, including tumor growth, migration and invasion. [ 9 ] Liu et al . [ 9 ] performed a microRNA array (GSE65071) between OS and normal tissue and found that miR‐874‐3p inhibits cell migration by targeting RGS4 in OS.…”

Section: Introductionmentioning

confidence: 99%

“…[ 9 ] Liu et al . [ 9 ] performed a microRNA array (GSE65071) between OS and normal tissue and found that miR‐874‐3p inhibits cell migration by targeting RGS4 in OS. According to previous reports, miR‐455‐3p is aberrantly expressed in pancreatic carcinoma, [ 10 ] colon adenocarcinoma [ 11 ] and glioma.…”

Section: Introductionmentioning

confidence: 99%

“…[2] The abnormal expression of miRNAs has recently been associated with several diseases, including tumor growth, migration and invasion. [9] Liu et al [9] performed a microRNA array (GSE65071) between OS and normal tissue and found that miR-874-3p inhibits cell migration by targeting RGS4 in OS. According to previous reports, miR-455-3p is aberrantly expressed in pancreatic carcinoma, [10] colon adenocarcinoma [11] and glioma.…”

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confidence: 99%

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LINC00319 promotes osteosarcoma progression by regulating the miR‐455‐3p/NFIB axis

Sun

et al. 2020

The Journal of Gene Medicine

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Background: Numerous studies have shown that aberrant expression of long noncoding RNAs (lncRNAs) is associated with the development and metastasis of osteosarcoma (OS). However, the role and function of LINC00319 with respect to regulating OS progression is unknown. The present study aimed to reveal the function and related mechanism of LINC00319 in OS. Methods: The expression of LINC00319, miR-455-3p and nuclear factor IB (NFIB) in OS cells and tissues was determined using a reverse transcriptase-polymerase chain reaction (PCR). The sublocalization of LINC00319 was predicted by the lncATLAS database (http://lncatlas.crg.eu) and RNA fluorescence in situ hybridization (FISH) was further performed to detect the subcellular localization of LINC00319. LINC00319, miR-455-3p and NFIB target sites were predicted by StarBase (http:// starbase.sysu.edu.cn/index.php) and validated using a dual luciferase reporter gene assay. We subsequently performed LINC00319 gain-and loss-of-function studies to define the role of LINC00319 in OS cell migration. Results: PCR results showed that lncRNA LINC00319 exhibited high expression in tumor cells and tissue. Moreover, LINC00319 was positioned in the cytoplasm, which was identified by FISH. Knockdown of lncRNA LINC00319/NFIB or overexpression of miR-455-3p blocked the migration of OS cells. In addition, the inhibitory effect of migration with the knockdown of lncRNA LINC00319 was partially blocked by administration of miR-455-3p inhibitor. Conclusions: lncRNA LINC00319 may promote OS progression by regulating the miR-455-3p/NFIB axis, which probably serves as an innovative potential indicator of prognosis and a target of therapy for OS. K E Y W O R D S LINC00319, miR-455-3p, NFIB, osteosarcoma 1 | INTRODUCTION Osteosarcoma (OS) has been considered the most commonly occurring primary osseous sarcoma diagnosed in childhood. [1] Previous epidemiological studies have reported that the incidence rate of OS is four or five patients per 1,000,000 persons and that it is the main morbidity among teenagers. [2] Current treatment for OS mainly includes a combination of chemotherapy and limb salvage surgery. [3] Despite the improvement in numerous treatment methods (chemotherapy and curative resection), the 5-year overall survival rate of Farui Sun and Ziliang Yu contributed equally to this work.

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miR‐874‐3p inhibits cell migration through targeting RGS4 in osteosarcoma

Cited by 21 publications

References 24 publications

Circ_0007142 downregulates miR‐874‐3p‐mediated GDPD5 on colorectal cancer cells

Circ_0007142 downregulates miR‐874‐3p‐mediated GDPD5 on colorectal cancer cells

Inhibition of lncRNA DCST1‐AS1 suppresses proliferation, migration and invasion of cervical cancer cells by increasing miR‐874‐3p expression

LINC00319 promotes osteosarcoma progression by regulating the miR‐455‐3p/NFIB axis

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