miR‐802 inhibits the proliferation, invasion, and epithelial‐mesenchymal transition of glioblastoma multiforme cells by directly targeting SIX4

H, Wu; Yu, Shaojun; Han, Bin; Wang, Qilong; Zhang, Bin; Qi, Chunjian; Liu, Fang

doi:10.1002/cbf.3451

Cited by 13 publications

(8 citation statements)

References 31 publications

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“…microRNA-802, located on the 21st chromosome, has been intensively studied in different types of cancer, and it has been shown that it plays a crucial role during metastasis, progression, and invasion. Huang et al [64] observed that miR-802 not only inhibits the proliferation and invasion but also the epithelial-mesenchymal transition of glioblastoma multiforme cells. miR-802's role has also been described in tongue squamous cell carcinoma [65], pancreatic cancer progression [66], and human cervical cancer [67].…”

Section: Mir-802mentioning

confidence: 99%

Non-Coding RNAs as Potential Novel Biomarkers for Early Diagnosis of Hepatic Insulin Resistance

Pielok

Marycz

2020

IJMS

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In the recent years, the prevalence of metabolic conditions such as type 2 Diabetes (T2D) and metabolic syndrome (MetS) raises. The impairment of liver metabolism resulting in hepatic insulin resistance is a common symptom and a critical step in the development of T2D and MetS. The liver plays a crucial role in maintaining glucose homeostasis. Hepatic insulin resistance can often be identified before other symptoms arrive; therefore, establishing methods for its early diagnosis would allow for the implementation of proper treatment in patients before the disease develops. Non-coding RNAs such as miRNAs (micro-RNA) and lncRNAs (long-non-coding RNA) are being recognized as promising novel biomarkers and therapeutic targets—especially due to their regulatory function. The dysregulation of miRNA and lncRNA activity has been reported in the livers of insulin-resistant patients. Many of those transcripts are involved in the regulation of the hepatic insulin signaling cascade. Furthermore, for several miRNAs (miR-802, miR-499-5p, and miR-122) and lncRNAs (H19 imprinted maternally expressed transcript (H19), maternally expressed gene 3 (MEG3), and metastasis associated lung adenocarcinoma transcript 1 (MALAT1)), circulating levels were altered in patients with prediabetes, T2D, and MetS. In the course of this review, the role of the aforementioned ncRNAs in hepatic insulin signaling cascade, as well as their potential application in diagnostics, is discussed. Overall, circulating ncRNAs are precise indicators of hepatic insulin resistance in the development of metabolic diseases and could be applied as early diagnostic and/or therapeutic tools in conditions associated with insulin resistance.

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Section: Mir-802mentioning

confidence: 99%

Non-Coding RNAs as Potential Novel Biomarkers for Early Diagnosis of Hepatic Insulin Resistance

Pielok

Marycz

2020

IJMS

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“…13 In addition, recent studies also showed that miR-802 acted as a tumor suppressor in gastric cancer, non-small cell lung cancer, epithelial ovarian cancer, laryngeal cancer, tongue squamous cell carcinoma and glioblastoma via, respectively, suppressing down-stream multiple oncogenes expression, including RAB23, FGFR1, YWHAZ, ARPP19, MAP2K4 or SIX4. 12,[15][16][17]20,24 Herein, our clinical results showed that miR-802 was significantly down-regulated in CRC tissues, and lower miR-802 expression was apparently associated with poorer tumor grade, later T stage, later N stage and advanced TNM stage and worse patient's OS. Furthermore, elevated expression of miR-802 suppressed CRC cells proliferation, migration and invasion while enhanced the apoptosis of CRC cells by directly targeting UBN2 both in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 76%

“…Numerous previous studies have indicated miR-802 was downregulation in multiple types of human cancers and played tumor suppressor role in cancer progression. 12,13,[15][16][17][18][19][20] Yuan et al firstly reported the inhibitory effect of miR-802 on cell proliferation via downregulation of FoxM1 in breast cancer; 18 Zhang and collogues found that miR-802 was obviously down-regulated in cervical cancer, which inhibited cell proliferation and induced apoptosis by targeting SRSF9; 19 Wang et al found miR-802 inhibited epithelial-mesenchymal transition (EMT), migration and invasion of prostate cancer cells through targeting Flot2. 13 In addition, recent studies also showed that miR-802 acted as a tumor suppressor in gastric cancer, non-small cell lung cancer, epithelial ovarian cancer, laryngeal cancer, tongue squamous cell carcinoma and glioblastoma via, respectively, suppressing down-stream multiple oncogenes expression, including RAB23, FGFR1, YWHAZ, ARPP19, MAP2K4 or SIX4.…”

Section: Discussionmentioning

confidence: 99%

“…[11][12][13][14][15][16][17][18][19][20] Noteworthily, the biological role of miR-802 in the tumorigenesis of human cancer mainly depends on cancer type. Numerous studies reported that miR-802 functions as a tumor suppressor in most cancers, including glioblastoma, 12 tongue squamous cell carcinoma, 15 laryngeal, 17 breast, 18 gastric, 20 prostate, 13 ovarian, 16 and cervical cancer. 19 Nevertheless, miR-802 was demonstrated to function as an onco-miRNA in lung carcinoma 14 and osteosarcoma.…”

Section: Introductionmentioning

confidence: 99%

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<p>MicroRNA-802 Suppresses Tumorigenesis of Colorectal Cancer via Regulating UBN2</p>

Yang¹,

Guo²,

Li³

et al. 2020

CMAR

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Background: The initiation and progression of colorectal cancer (CRC) are a multistep complex process regulated by multiple factors. Previous evidence indicated that microRNA-802 (miR-802) participated in tumorigenesis of numerous solid cancers; however, the potential roles and underlying mechanisms of miR-802 in CRC still need further exploration. Methods: Quantitative real-time PCR (qRT-PCR) was employed to evaluate miR-802 levels in human CRC tissues and cell lines. In vitro proliferation, apoptosis, migration and invasion assays, and in vivo subcutaneous mouse xenograft model were utilized to examine the effects of miR-802 on the malignant behaviors of CRC cells. Then, bioinformatics prediction, dualluciferase reporter, qRT-PCR, and Western blot was conducted to confirm the downstream target of miR-802. Results: MiR-802 was frequently down-regulated in CRC tissues and cells. Further analyses showed that the low expression of miR-802 in CRC tissues was significantly correlated with tumor progression and poor patients' prognosis. Overexpression of miR-802 profoundly inhibited proliferation, migration and invasion but promoted apoptosis of CRC cells, by contrast, miR-802 silencing exhibited opposite effects in vitro. Further animal experiment demonstrated that miR-802 could suppress tumor growth via inhibiting the proliferation and promoting the apoptosis of CRC cells in vivo. Mechanistically, miR-802 functioned as a tumor suppressor through inhibiting the expression of Ubinuclein-2 (UBN2) on posttranscriptional level. Moreover, upregulation of UBN2 expression could reverse the biological effects of CRC cells induced by miR-802 overexpression. Conclusion: Our study demonstrates that miR-802 inhibits the proliferation, migration and invasion while promotes the apoptosis of CRC cells via directly suppressing UBN2 expression. These findings provide a promising biomarker and potential treatment target for CRC.

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“…In addition, SIX4 has been found to act as a master regulator of oncogenes that promotes tumorigenesis in NSCLC cells 19 . Wu et al found that knockdown of SIX4 inhibited cell growth, invasion, and the EMT of glioblastoma 20 . Moreover, it has been reported that downregulation of SIX4 led to bladder cancer cell cycle arrest and apoptosis 21 .…”

Section: Discussionmentioning

confidence: 99%

Upregulation of SIX4 indicates poor clinical outcome and promotes tumor growth and cell metastasis in esophageal squamous cell carcinoma

Jiang²,

Yan

et al. 2021

Thoracic Cancer

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Background The role of sine oculis homeobox 4 (SIX4) has been found in some malignant tumors. However, there have been few studies on the function of SIX4 in esophageal squamous cell carcinoma (ESCC). This study aimed to explore the regulatory mechanism of SIX4 in ESCC. Methods RT‐qPCR and Western blot analysis were used to measure mRNA and protein expression. The function of SIX4 was investigated using CCK‐8, colony formation, flow cytometry, wound healing and transwell assays. A mouse xenograft tumor assay was designed to perform in vivo experiments. Results SIX4 was upregulated in ESCC and indicated poor clinical outcomes in ESCC patients. Functionally, knockdown of SIX4 inhibited cell proliferation and induced apoptosis in ESCC. In addition, the silencing of SIX4 inhibited cell migration, invasion and EMT in ESCC. More importantly, upregulation of SIX4 could activate the PI3K/AKT pathway in ESCC cells and promote tumor growth in vivo. Conclusions Upregulation of SIX4 indicates poor clinical outcomes in ESCC patients and promotes tumor growth and cell metastasis in ESCC.

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miR‐802 inhibits the proliferation, invasion, and epithelial‐mesenchymal transition of glioblastoma multiforme cells by directly targeting SIX4

Cited by 13 publications

References 31 publications

Non-Coding RNAs as Potential Novel Biomarkers for Early Diagnosis of Hepatic Insulin Resistance

Non-Coding RNAs as Potential Novel Biomarkers for Early Diagnosis of Hepatic Insulin Resistance

<p>MicroRNA-802 Suppresses Tumorigenesis of Colorectal Cancer via Regulating UBN2</p>

Upregulation of SIX4 indicates poor clinical outcome and promotes tumor growth and cell metastasis in esophageal squamous cell carcinoma

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