MiR-760 overexpression promotes proliferation in ovarian cancer by downregulation of PHLPP2 expression

Liao, Yanbin; Deng, Ying; Li, Jun; Ye, Zhanying; Zhang, You; Yao, Shuzhong; He, Shanyang

doi:10.1016/j.ygyno.2016.09.010

Cited by 50 publications

(43 citation statements)

References 38 publications

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“…In OVA, we validated that the miR‐760 expression is markedly upregulated in OVA tissues and cell lines, and high miR‐760 expression is correlated with poor prognosis and an aggressive phenotype in OVA patients. miR‐760 upregulation promotes, whereas miR‐760 downregulation inhibits the OVA cell proliferation in vitro . This study also adivsed that miR‐760 downregulation suppressed the OVA cell migration and proliferation.…”

Section: Discussionsupporting

confidence: 63%

Upregulation of hsa_circ_0007874 suppresses the progression of ovarian cancer by regulating the miR‐760/SOCS3 pathway

Yu²,

Zhang

et al. 2020

Cancer Medicine

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Ovarian cancer (OVA) is a fatal and common malignancy in women worldwide. Circular RNAs (circRNAs) consist of a family of circular endogenous RNAs generated by selective splicing, and they are involved in many diseases. Previous studies reported that hsa_circ_0007874 is aberrantly expressed in cancer and functions in tumorigenesis. While the hsa_circ_0007874 role in OVA is unclear. Here, we detected the hsa_circ_0007874 expression in OVA cell lines using Rt‐qPCR. Hsa_circ_0007874 subcellular localization was confirmed by fluorescence in situ hybridization. The relationship between hsa_circ_0007874, microRNAs (miRNAs), and relative protein levels was assessed using the luciferase reporter assays. Results verified that hsa_circ_0007874 is downregulated in OVA cell lines. hsa_circ_0007874 overexpression decreased the OVA cell migration and proliferation in vitro and in vivo. Bioinformatics and luciferase reporter assays confirmed that miR‐760 and SOCS3 are the downstream targets of hsa_circ_0007874. Downregulation of SOCS3 or miR‐760 overexpression restored the migration and proliferation ability of SKOV3 or A2780 cells overexpressing hsa_circ_0007874. Downregulation of SOCS3 restored the proliferation and migration in miR‐760 knockdown SKOV3 and A2780 cells. In summary, the data suggest that hsa_circ_0007874 acts as a tumor suppressor by regulating the miR‐760/SOCS3 axis, highlighting its potential as an effective therapeutic target for OVA.

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Section: Discussionsupporting

confidence: 63%

Upregulation of hsa_circ_0007874 suppresses the progression of ovarian cancer by regulating the miR‐760/SOCS3 pathway

Yu²,

Zhang

et al. 2020

Cancer Medicine

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“…As an identified tumor suppressive gene, multiple lines of evidence reveal the role of PHLPP2 in tumor progression . PHLPP2 is involved in the inhibition of malignant behaviors of cancer attributed to the suppression of prosurvival signaling such as through the PI3K/Akt signaling pathway .…”

Section: Discussionmentioning

confidence: 99%

“…Maintaining balanced levels of PHLPP2 expression is essential in preventing pathologies, as changes in steady‐state levels of PHLPP2 are correlated with many diseases including diabetes, hepatic steatosis and cancer . Recently, many studies have shown that PHLPP2 expression is ubiquitously lost in multiple cancers and plays a key role in a wide range of biological processes such as cancer cell proliferation, metastasis, autophagy and apoptosis …”

Section: Introductionmentioning

confidence: 99%

PHLPP2 as a novel metastatic and prognostic biomarker in non‐small cell lung cancer patients

Wang

Tian

et al. 2019

Thoracic Cancer

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Background PH domain and leucine‐rich repeat protein phosphatase 2 (PHLPP2) has been reported to be a potent tumor suppressor in many human cancers. However, PHLPP2 has not been fully researched as a putative clinical prognostic biomarker of lung cancer. Methods The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases including data on 1383 non‐small cell lung cancer (NSCLC) patients were used to determine PHLPP2 expression. PHLPP2 expression was then examined by immunohistochemistry, and its clinical significance analyzed in 134 NSCLC patients, including 73 patients with adenocarcinoma and 81 with squamous cell carcinoma. Results We found PHLPP2 expression to be less pronounced in NSCLC tissue samples than that in nontumoral lung tissues according to data taken from TCGA and GEO datasets; this outcome was further validated by immunohistochemistry assay. The low PHLPP2 expression level was found to be associated with the presence of lymph node metastasis (P = 0.003). Importantly, PHLPP2 was found to be an independent indicator of prognosis for overall (hazard ratio [HR] = 0.520, 95% confidence interval [Cl] = 0.327–0.827; P = 0.006) and disease‐free survival (HR = 0.489, 95% Cl = 0.308–0.775; P = 0.002) in patients with surgically‐resected NSCLC by multivariate analysis. Conclusion Taken together, our findings show that PHLPP2 is a robust clinical marker for NSCLC survival and could serve as a potential therapeutic target.

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“…17 But in recent studies showed that miR-760 may act as a promoter in ovarian cancer and hepatocellular cancer. 26,27 These evidence showed the concept that miRNAs play a dual role as oncomirs and anti-oncomirs in various tumors.…”

Section: Discussionmentioning

confidence: 91%

“…Previous reports, such as miR‐760 has been involved in the early diagnosis of colorectal cancer, and mediates chemoresistance through inhibition of EMT in breast cancer cells, indicated that miR‐760 functions as suppressor involved in the progression of multiple human tumors including hepatocellular cancer, lung cancer, and gastric cancer . But in recent studies showed that miR‐760 may act as a promoter in ovarian cancer and hepatocellular cancer . These evidence showed the concept that miRNAs play a dual role as oncomirs and anti‐oncomirs in various tumors.…”

Section: Discussionmentioning

confidence: 97%

miR‐760 exerts an antioncogenic effect in esophageal squamous cell carcinoma by negatively driving fat metabolism via targeting c‐Myc

Yang

Zhang

Tie

et al. 2019

J of Cellular Biochemistry

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miR‐760 is downregulated in various human tumors, and fat metabolism disorder correlates with tumor progression, especially anomalism of key fat metabolic enzymes that are positively modulated by c‐Myc. The aim of our study is to elucidate the presumptive molecular mechanisms of miR‐760‐mediated esophageal squamous cell carcinoma (ESCC) cell function and to assess the therapeutic significance of miR‐760 in ESCC patients. Quantitative real‐time PCR (RT‐qPCR) analysis indicated that miR‐760 was significantly downexpressed in ESCC tissues and cell lines. Cell counting kit‐8 (CCK‐8) assay, colony formation assay, transwell assay, and flow cytometry denoted that induced ectopic overexpression of miR‐760 dramatically inhibited ESCC cells proliferation, attenuated migration, and invasion facilitated apoptosis in vitro. Mechanistically, c‐Myc predicted using bioinformatics was identified as a potential target gene of miR‐760 by luciferase reporter assay. Furthermore, mRNA and protein expression levels of c‐Myc and key fat metabolic enzymes were downregulated with miR‐760 mimics. The above investigation results, responsible for the antineoplastic properties of miR‐760 in ESCC, preliminarily highlighted that the hypothetical signal amongst miR‐760, c‐Myc, and key fat metabolic enzymes may develop a novel diagnostic marker, therapeutic target, and independent prognostic indicator.

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MiR-760 overexpression promotes proliferation in ovarian cancer by downregulation of PHLPP2 expression

Cited by 50 publications

References 38 publications

Upregulation of hsa_circ_0007874 suppresses the progression of ovarian cancer by regulating the miR‐760/SOCS3 pathway

Upregulation of hsa_circ_0007874 suppresses the progression of ovarian cancer by regulating the miR‐760/SOCS3 pathway

PHLPP2 as a novel metastatic and prognostic biomarker in non‐small cell lung cancer patients

miR‐760 exerts an antioncogenic effect in esophageal squamous cell carcinoma by negatively driving fat metabolism via targeting c‐Myc

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