miR-709 inhibits GHRP6 induced GH synthesis by targeting PRKCA in pituitary

Cheng, Yi; Chen, Ting; Song, Jié; Qi, Qien; Wang, Chunli; Xi, Qianyun; Liu, Songcai; Hao, Linlin; Zhang, Yongliang

doi:10.1016/j.mce.2020.110763

Cited by 11 publications

(12 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results above suggest that rno_circ_0001004 is a molecular sponge for miR-709. Our previous studies demonstrated that miR-709 significantly inhibited the GH synthesis by targeting PKCA [12]. Thus, we further detected the change in PKCα pathway and GH expression and release.…”

Section: Rno_circ_0001004 Antagonizes Mir-709-mediated Repression Of Gh Synthesis and Secretionmentioning

confidence: 78%

“…Our previous study showed that miR-709 suppressed the viability of GH3 cells [12]. Thus, we performed CCK8 and EdU assays to determine the effect of rno_circ_0001004 on GH3 cell proliferation.…”

Section: Rno_circ_0001004 Promoted the Viability Of Gh3 Cellsmentioning

confidence: 99%

“…More and more evidence has demonstrated that miRNA functions broadly in development, physiology, and pathology by influencing cell proliferation, cell differentiation, cell migration, apoptosis, metabolism and signal transduction [6][7][8][9][10][11]. We have previously shown that the miR-709 is highly expressed in the pituitary and inhibits the GH synthesis and suppresses the viability of GH 3 cells [12] by targeting Protein Kinase C alpha (PKCA). Protein Kinase C (PKC) is a class of phospholipid-dependent kinases that participate in regulation of protein secretion including GH and luteinizing hormone (LH) [13,14], as well as regulating cell proliferation [15].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rno_circ_0001004 Acts as a miR-709 Molecular Sponge to Regulate the Growth Hormone Synthesis and Cell Proliferation

Xiong

Zhang

Wang

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

(1) Background: As a novel type of non-coding RNA with a stable closed-loop structure, circular RNA (circRNA) can interact with microRNA (miRNA) and influence the expression of miRNA target genes. However, circRNA involved in pituitary growth hormone (GH) regulation is poorly understood. Our previous study revealed protein kinase C alpha (PRKCA) as the target gene of miR-709. Currently, the expression and function of rno_circRNA_0001004 in the rat pituitary gland is not clarified; (2) Methods: In this study, both bioinformatics analysis and dual-luciferase report assays showed a target relationship between rno_circRNA_0001004 and miR-709. Furthermore, the rno_circRNA_0001004 overexpression vector and si-circ_0001004 were constructed and transfected into GH3 cells; (3) Results: We found that rno_circRNA_0001004 expression was positively correlated with the PRKCA gene and GH expression levels, while it was negatively correlated with miR-709. In addition, overexpression of rno-circ_0001004 also promoted proliferation and relieved the inhibition of miR-709 in GH3 cells; (4) Conclusions: Our findings show that rno_circ_0001004 acts as a novel sponge for miR-709 to regulate GH synthesis and cell proliferation, and are the first case of discovery of the regulatory role of circRNA_0001004 in pituitary GH.

show abstract

Section: Rno_circ_0001004 Antagonizes Mir-709-mediated Repression Of Gh Synthesis and Secretionmentioning

confidence: 78%

Section: Rno_circ_0001004 Promoted the Viability Of Gh3 Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rno_circ_0001004 Acts as a miR-709 Molecular Sponge to Regulate the Growth Hormone Synthesis and Cell Proliferation

Xiong

Zhang

Wang

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The Ct values of the target genes were normalized by the Ct value of the β-actin gene. The 2 –ΔCt method was employed to quantify and normalize the expression data ( Cheng et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Synonymous Mutations of Porcine Igf1r Extracellular Domain Affect Differentiation and Mineralization in MC3T3-E1 Cells

Wang

Liu

et al. 2020

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

Owing to the wide application of miniature pigs in biomedicine, the formation mechanism of its short stature must be elucidated. The insulin-like growth factor 1 receptor (IGF-1R), which receives signals through the extracellular domain (ECD) binding with ligands, is crucial in regulating cell growth and bone matrix mineralization. In this study, two haplotypes of Igf1r with four synonymous mutations in the coding sequences of IGF-1R ECD between large pigs (LP) and Bama pigs (BM) were stably expressed in the Igf1r-knockout MC3T3-E1 cells and named as MC3T3-LP cells (LP group) and MC3T3-BM cells (BM group), respectively. IGF-1R expression was lower in the BM group than in the LP group both in terms of transcription and translation levels, and IGF-1R expression inhibited cell proliferation. In addition, IGF-1R expression in the BM group promoted early-stage differentiation but delayed late-stage differentiation, which not only suppressed the expression of bone-related factors but also reduced alkaline phosphatase activity and calcium deposition. Moreover, different haplotypes of Igf1r changed the stability and conformation of the protein, further affecting the binding with IGF-1. Our data indicated that the four synonymous mutations of IGF1R ECD encoded by affect gene transcription and translation, thereby further leading to differences in the downstream pathways and functional changes of osteoblasts.

show abstract

“…Several miRNAs regulate the growth plate and GH-IGF axis, contributing to longitudinal growth. For example, miR-709 inhibits GHRP6-induced GH synthesis by targeting PRKCA in the pituitary [209, 210]. There are indications that miRNAs are also involved in catch-up growth in children born SGA [211].…”

Section: Hypothetical Causes Of Short Stature Associated With a Low Serum Igf-i And Normal Stimulated Gh Peakmentioning

confidence: 99%

Differential Diagnosis of the Short IGF-I-Deficient Child with Apparently Normal Growth Hormone Secretion

et al. 2021

View full text Add to dashboard Cite

The current differential diagnosis for a short child with low insulin-like growth factor I (IGF-I) and a normal growth hormone (GH) peak in a GH stimulation test (GHST), after exclusion of acquired causes, includes the following disorders: (1) a decreased spontaneous GH secretion in contrast to a normal stimulated GH peak (“GH neurosecretory dysfunction,” GHND) and (2) genetic conditions with a normal GH sensitivity (e.g., pathogenic variants of GH1 or GHSR) and (3) GH insensitivity (GHI). We present a critical appraisal of the concept of GHND and the role of 12- or 24-h GH profiles in the selection of children for GH treatment. The mean 24-h GH concentration in healthy children overlaps with that in those with GH deficiency, indicating that the previously proposed cutoff limit (3.0–3.2 μg/L) is too high. The main advantage of performing a GH profile is that it prevents about 20% of false-positive test results of the GHST, while it also detects a low spontaneous GH secretion in children who would be considered GH sufficient based on a stimulation test. However, due to a considerable burden for patients and the health budget, GH profiles are only used in few centres. Regarding genetic causes, there is good evidence of the existence of Kowarski syndrome (due to GH1 variants) but less on the role of GHSR variants. Several genetic causes of (partial) GHI are known (GHR, STAT5B, STAT3, IGF1, IGFALS defects, and Noonan and 3M syndromes), some responding positively to GH therapy. In the final section, we speculate on hypothetical causes.

show abstract

miR-709 inhibits GHRP6 induced GH synthesis by targeting PRKCA in pituitary

Cited by 11 publications

References 62 publications

Rno_circ_0001004 Acts as a miR-709 Molecular Sponge to Regulate the Growth Hormone Synthesis and Cell Proliferation

Rno_circ_0001004 Acts as a miR-709 Molecular Sponge to Regulate the Growth Hormone Synthesis and Cell Proliferation

Synonymous Mutations of Porcine Igf1r Extracellular Domain Affect Differentiation and Mineralization in MC3T3-E1 Cells

Differential Diagnosis of the Short IGF-I-Deficient Child with Apparently Normal Growth Hormone Secretion

Contact Info

Product

Resources

About