MiR-613: a novel diagnostic and prognostic biomarker for patients with esophageal squamous cell carcinoma

Guan, Shanghui; Chen, Xuan; Liu, Bowen; Tan, Bingxu; Liu, Fang; Wang, Ding; Han, Lihui; Wang, Lu; Huang, Xiaochen; Yao, Bin; Shi, Jialei; Chen, Pengxiang; Nesa, Effat Un; Song, Qingxu; Cheng, Yufeng

doi:10.1007/s13277-015-4271-8

Cited by 27 publications

(19 citation statements)

References 28 publications

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“…MiR-613 is reported to be downregulated in several types of cancer including prostate and ovarian cancer and esophageal squamous cell carcinoma [26–28]. In this study, we confirmed that miR-613 was downregulated in PTC.…”

Section: Discussionsupporting

confidence: 83%

MicroRNA-613 inhibits cell growth, migration and invasion of papillary thyroid carcinoma by regulating SphK2

et al. 2016

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MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized as key players in carcinogenesis. In this study, we investigated the biological function and mechanism of miR-613 in the regulation of papillary thyroid cancer (PTC) development. We found that miR-613 was downregulated in PTC cell lines and tissues, and overexpression of miR-613 significantly suppressed PTC cell growth, migration and invasion in vitro and inhibited tumor growth in vivo. We identified the gene for sphingosine kinase 2 (SphK2) as a direct target of miR-613. Overexpression of miR-613 significantly repressed SphK2 expression by directly targeting its 3′-untranslated regions (3′-UTR) and restoration of SphK2 reversed the inhibitory effects of miR-613 on PTC cell growth and invasion. Taken together, our results indicated that miR-613 functions as a tumor suppressor in PTC and its suppressive effect is mediated by repressing SphK2 expression.

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Section: Discussionsupporting

confidence: 83%

MicroRNA-613 inhibits cell growth, migration and invasion of papillary thyroid carcinoma by regulating SphK2

et al. 2016

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“…Previous studies have reported that miR-613 functions as a tumor suppressor in human cancers, including glioma (12), hepatocellular carcinoma (13), non-small cell lung cancer (14) and laryngeal squamous cell carcinoma (15). In ESCC, miR-613 has been identified as a diagnostic and prognostic biomarker for patients (16). However, the molecular mechanism underlying the action of miR-613 in ESCC remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

miR‑613 suppresses migration and invasion in esophageal squamous cell carcinoma via the targeting of G6PD

Gao

Feng

et al. 2020

Exp Ther Med

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Esophageal squamous cell carcinoma (ESCC) is a common cancer in China and has a high mortality rate. MicroRNAs (miRs) are a family of post-transcriptional regulators, which negatively regulate target gene expression. miR-613 has been revealed to be a diagnostic and prognostic biomarker in ESCC. However, the role of miR-613 in ESCC remains unclear. In the present study, miR-613 expression was identified to be reduced in tumor tissues in comparison with corresponding adjacent normal tissues. TargetScan and a dual-luciferase reporter assay verified glucose-6-phosphate dehydrogenase (G6PD) as a direct target of miR-613. In contrast with miR-613, G6PD expression was increased in tumor tissues compared with matched healthy tissues. Furthermore, overexpression of miR-613 inhibited cell migration and invasion of Eca109 cells compared with controls, while G6PD overexpression reversed the inhibition induced by miR-613, as determined by wound healing and Transwell assays. In addition, miR-613 overexpression decreased the mRNA and protein expression of G6PD, matrix metalloproteinase (MMP)2 and MMP9, and reduced the phosphorylation of signal transducer and activator of transcription 3 (STAT3) compared with controls, while G6PD reversed the effects of miR-613. However, miR-613 and G6PD did not affect the expression of STAT3. In conclusion, the aforementioned results suggest that miR-613 targets G6PD to suppress ESCC cell migration and invasion through reduced MMP2 and MMP9 expression and inactivation of the STAT3 signaling pathway. Thus, the present study may provide a new molecular foundation for treatment of ESCC.

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“…To date, various studies have proposed a tumor-suppressive role of miR-613 in many types of cancers. Decreased expression of miR-613 has been suggested as novel diagnostic and prognostic biomarker for esophageal squamous-cell carcinoma (31) and ovarian cancer (32). Fu et al (33) reported that miR-613 suppressed ovarian cancer cell proliferation and invasion through targeting of KRAS.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-613 inhibits proliferation and invasion of renal cell carcinoma cells through targeting FZD7

Song

Nan

Wang

et al. 2017

Molecular Medicine Reports

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MicroRNAs (miRNAs) have emerged as critical regulators in cancer progression. miR‑613 has been reported as a tumor suppressor gene in many types of human cancers. However, the function of miR‑613 in renal cell carcinoma (RCC) remains unclear. In the present study, the authors aimed to detect the expression of miR‑613 and its function in RCC cell lines. miR‑613 was reported to be significantly downregulated RCC cell lines. Functional analyses demonstrated that overexpression of miR‑613 significantly decreased RCC cell proliferation and invasion. Bioinformatics analysis showed that Frizzled7 (FZD7) was a predicted target of miR‑613, which was verified by dual‑luciferase reporter assay, reverse transcription quantitative‑polymerase chain reaction and western blot analysis. Restoration of FZD7 significantly reversed the suppressive effects of miR‑613 on RCC cell proliferation and invasion. Taken together, the results of the present study indicated that miR‑613 functions as a tumor suppressor that inhibits RCC cell proliferation and invasion by targeting and inhibiting FZD7, providing novel insight into RCC pathogenesis and a potential therapeutic target for RCC.

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MiR-613: a novel diagnostic and prognostic biomarker for patients with esophageal squamous cell carcinoma

Cited by 27 publications

References 28 publications

MicroRNA-613 inhibits cell growth, migration and invasion of papillary thyroid carcinoma by regulating SphK2

MicroRNA-613 inhibits cell growth, migration and invasion of papillary thyroid carcinoma by regulating SphK2

miR‑613 suppresses migration and invasion in esophageal squamous cell carcinoma via the targeting of G6PD

MicroRNA-613 inhibits proliferation and invasion of renal cell carcinoma cells through targeting FZD7

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