MicroRNA-613 inhibits proliferation and invasion of renal cell carcinoma cells through targeting FZD7

Song, Hongjiang; Nan, Yong-Hao; Wang, Xinsheng; Zhang, Gang; Zong, Shi; Kong, Xiangbo

doi:10.3892/mmr.2017.7076

Cited by 11 publications

(7 citation statements)

References 41 publications

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“… 14 miR-613 has been demonstrated to be downregulated in several types of cancers including renal cell carcinoma, glioma, breast cancer, and cervical cancer, implying that miR-613 may act as a tumor suppressor gene. 15 - 18 However, its function and mechanism in PC remain unclear.…”

Section: Introductionmentioning

confidence: 99%

CircCCT3 Acts as a Sponge of miR-613 to Promote Tumor Growth of Pancreatic Cancer Through Regulating VEGFA/VEGFR2 Signaling

et al. 2021

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Background: Circular RNAs (CircRNAs) have been recently implicated in the progression of pancreatic cancer (PC). Aims: To investigate the involvement of CircCCT3 in PC and studying its interactions and functioning during the progression of PC in vitro and in vivo, using methods of molecular biology and bioinformatics. Study Desig n: Experimental study. Methods: The expressions of CircCCT3 and miR-613 in pancreatic carcinoma tissues and cell lines were evaluated by quantitative real-time polymerase chain reaction (PCR). The relationship between clinical pathologic features as well as the survival rate and CircCCT3 expression was analyzed with chi-square test and the Kaplan–Meier method. CCK-8, wound healing, transwell assays, and the fluorescein isothiocyanate-AnnexinV/propidium iodide (FITC-AnnexinV/PI) assay were used to assess cell proliferation, migration, invasion, and apoptosis after CircCCT3 overexpression or downregulation. The Dual-Luciferase reporter assay, RNA immunoprecipitation (RIP), RNA pull-down and fluorescence in situ hybridization (FISH) assays were performed to validate the potential interaction of CircCCT3, miR-613, and vascular endothelial growth factor (VEGFA). The nude mouse xenograft tumor assay was used to detect CircCCT3 effects on pancreatic tumorigenesis in vivo. Western blotting analysis was performed to examine the VEGFA and the vascular endothelial growth factor receptor 2 (VEGFR2) protein expressions following. Results: CircCCT3 expression was significantly increased in PC tissues (3.41 ± 0.57 vs. 1.00 ± 0.10, P < .01) and cell lines (Patu8988 2.57 ± 0.20; SW1990 2.88 ± 0.10; BxPC-3 2.45 ± 0.20; Panc02 2.99 ± 0.10 vs. H6c7 1.00 ± 0.10; all P < .001). CircCCT3 expression was negatively correlated with miR-613 expression. PC patients with high CircCCT3 expression exhibited significantly poorer overall survival rate than those patients with low CircCCT3 expression ( P = .013). Moreover, it was found that CircCCT3 promoted cell proliferation, migration, and invasion, and inhibited cell apoptosis in PC cells. The CircCCT3 acted as a sponge for the miR-613 to facilitate VEGFA and VEGFR2 expression. si-CirCCT3 also inhibited tumor growth of PC in nude mice. si-CircCCT3 reduced VEGFA and VEGFR2 expression, whereas overexpression of CircCCT3 increased VEGFA and VEGFR2 expression. Conclusion: Increased CircCCT3 suggests a poor prognosis for PC patients and promotes the migration and invasion through targeting VEGFA/VEGFR2 signaling. CircCCT3 may serve as a potential and promising therapeutic target for PC treatment.

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Section: Introductionmentioning

confidence: 99%

CircCCT3 Acts as a Sponge of miR-613 to Promote Tumor Growth of Pancreatic Cancer Through Regulating VEGFA/VEGFR2 Signaling

et al. 2021

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“…10 miR-613 has been demonstrated to be downregulated in several types of cancers including renal cell carcinoma, glioma, lung cancer, and cervical cancer, implying that miR-613 may act as a tumor suppressor gene. [11][12][13][14] For instance, miR-613 targeted CDK9 to inhibit proliferation and invasion. 15 It was also reported that downregulation of miR-613 facilitated liver cancer stem cells, indicating that miR-613 was involved in tumor stemness.…”

Section: Introductionmentioning

confidence: 99%

<p>miR-613 Suppresses Chemoresistance and Stemness in Triple-Negative Breast Cancer by Targeting FAM83A</p>

Liu

Jiang

Han

2020

CMAR

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“…Almost all members of the Wnt receptor family, such as FZD4, 6, 7, 8, and 10 have been reported to be regulated by different miRNAs in multiple cancer types including colorectal cancer, prostate cancer, breast cancer (Gong et al, 2014;Kim B.-K. et al, 2015;Jiang et al, 2016;Song et al, 2017;Li Z.-T. et al, 2020). In a few cases, upregulation of miRNAs regulating the negative (Kim et al, 2011;Hata et al, 2017;Poodineh et al, 2020).…”

Section: Receptorsmentioning

confidence: 99%

Epigenetic Regulation of the Wnt/β-Catenin Signaling Pathway in Cancer

2021

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Studies over the past four decades have elucidated the role of Wnt/β-catenin mediated regulation in cell proliferation, differentiation and migration. These processes are fundamental to embryonic development, regeneration potential of tissues, as well as cancer initiation and progression. In this review, we focus on the epigenetic players which influence the Wnt/β-catenin pathway via modulation of its components and coordinated regulation of the Wnt target genes. The role played by crosstalk with other signaling pathways mediating tumorigenesis is also elaborated. The Hippo/YAP pathway is particularly emphasized due to its extensive crosstalk via the Wnt destruction complex. Further, we highlight the recent advances in developing potential therapeutic interventions targeting the epigenetic machinery based on the characterization of these regulatory networks for effective treatment of various cancers and also for regenerative therapies.

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MicroRNA-613 inhibits proliferation and invasion of renal cell carcinoma cells through targeting FZD7

Cited by 11 publications

References 41 publications

CircCCT3 Acts as a Sponge of miR-613 to Promote Tumor Growth of Pancreatic Cancer Through Regulating VEGFA/VEGFR2 Signaling

CircCCT3 Acts as a Sponge of miR-613 to Promote Tumor Growth of Pancreatic Cancer Through Regulating VEGFA/VEGFR2 Signaling

<p>miR-613 Suppresses Chemoresistance and Stemness in Triple-Negative Breast Cancer by Targeting FAM83A</p>

Epigenetic Regulation of the Wnt/β-Catenin Signaling Pathway in Cancer

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