miR-612 suppresses stem cell-like property of hepatocellular carcinoma cells by modulating Sp1/Nanog signaling

Yang, Liu; Liu, Dongli; Dong, Lili; Wen, Duo; Shi, Dongmin; Zhou, Jian; Fan, Jia; Wu, Wei‐Zhong

doi:10.1038/cddis.2016.282

Cited by 44 publications

(37 citation statements)

References 47 publications

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“…Further studies on the regulatory function of miRNAs and their potential molecular mechanisms in cervical cancer may aid in identifying diagnostic markers and therapeutic targets. MiR-612 has been shown to exhibit tumour-suppressing activity in multiple cancers by regulating major tumour-related biological behaviours [11][12][13][14][15][16][17]. However, the expression status and associated mechanisms of miR-612 in cervical cancer remain unclear.…”

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confidence: 99%

RETRACTED: MicroRNA‐612 inhibits cervical cancer progression by targeting NOB1

Yang

Zhou

Yao

et al. 2020

J Cellular Molecular Medi

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Recently, many studies have shown that microRNA (miR)‐612 is involved in cancer progression. However, the role of miR‐612 in cervical cancer remains unclear. The present study aims to investigate the biological effects of miR‐612 on cervical cancer. The expression of miR‐612 in cervical cancer tissues and cell lines was analysed by quantitative reverse transcription‐polymerase chain reaction. The effect of miR‐612 cell proliferation, migration, invasion and apoptosis was studied by appropriate methods. Protein expression was determined by Western blot analyses. Bioinformatics analysis and luciferase reporter assays were performed to clarify the relationship between miR‐612 and nin one binding protein (NOB1). A xenograft model was established to examine the role of miR‐612 in vivo tumorigenesis. Cervical cancer tissues and cell lines showed down‐regulation of miR‐612 expression, which was associated with the Fédération Internationale de Gynécologie et d'Obstétrique (FIGO) stage and lymph node metastasis. Functional assays revealed that miR‐612 overexpression significantly suppressed cervical cancer cell proliferation, migration and invasion in vitro and delayed tumour growth in vivo. Mechanically, miR‐612 targeted NOB1 in cervical cancer cells, revealing a negative correlation between miR‐612 and NOB1in cervical cancer samples. NOB1 overexpression partially reversed the inhibitory effects of miR‐612 overexpression in cervical cancer cells. Taken together, these findings indicate that miR‐612 functions as a tumour suppressor in cervical cancer and suggest that miR‐612 may be a potential target in the therapeutic intervention of this malignancy.

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confidence: 99%

RETRACTED: MicroRNA‐612 inhibits cervical cancer progression by targeting NOB1

Yang

Zhou

Yao

et al. 2020

J Cellular Molecular Medi

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“…Consequently, the expression of miR-612 decreases the amount of CSC stem markers and attenuates CSC-like properties in HCC [138]. miR-612, as a novel biomarker, deserves more study as an miRNA-targeting cancer therapy [139][140][141].…”

Section: Mir-612mentioning

confidence: 99%

microRNA: The Impact on Cancer Stemness and Therapeutic Resistance

Jiao

Qian

et al. 2019

Cells

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Cancer ranks as the second leading cause of death worldwide, causing a large social and economic burden. However, most anti-cancer treatments face the problems of tumor recurrence and metastasis. Therefore, finding an effective cure for cancer needs to be solved urgently. Recently, the discovery of cancer stem cells (CSCs) provides a new orientation for cancer research and therapy. CSCs share main characteristics with stem cells and are able to generate an entire tumor. Besides, CSCs usually escape from current anti-cancer therapies, which is partly responsible for tumor recurrence and poor prognosis. microRNAs (miRNAs) belong to small noncoding RNA and regulate gene post-transcriptional expression. The dysregulation of miRNAs leads to plenty of diseases, including cancer. The aberrant miRNA expression in CSCs enhances stemness maintenance. In this review, we summarize the role of miRNAs on CSCs in the eight most common cancers, hoping to bridge the research of miRNAs and CSCs with clinical applications. We found that miRNAs can act as tumor promoter or suppressor. The dysregulation of miRNAs enhances cell stemness and contributes to tumor metastasis and therapeutic resistance via the formation of feedback loops and constitutive activation of carcinogenic signaling pathways. More importantly, some miRNAs may be potential targets for diagnosis, prognosis, and cancer treatments.

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“…Contrastingly, miR-612 was found to be downregulated in warts compared to normal skin. miR-612 plays a critical role in the development of colorectal cancer cells, and it was also found to have a suppressive role in reducing the stemness and tumor metastasis of hepatocellular carcinoma cells [55,56]. In hepatocellular carcinoma patients, miR-612 inhibits the metastasis and epithelial-mesenchymal transition of cancer cells by targeting the AKT2 gene [57].…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profiling of MicroRNAs in HPV-Induced Warts and Normal Skin

et al. 2019

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Infection with the human papillomavirus (HPV) is a common occurrence among the global population, with millions of new cases emerging on an annual basis. Dysregulated microRNA (miRNA) expression is increasingly being identified to play a role in a number of different diseases, especially in the context of high-risk HPV infection. The present study investigated the miRNA expression profiles of warts induced by low-risk HPV. In warts, miR-27b, miR-24-1, miR-3654, miR-647, and miR-1914 were downregulated while miR-612 was upregulated compared to normal skin. Using miRTargetLink Human, experimentally supported evidence was obtained showing that miR-27b targeted the vascular endothelial growth factor C (VEGFC) and CAMP-responsive element binding protein 1 (CREB1) genes. The VEGFC and CREB1 genes have been reported to be involved in tumorigenesis and wart formation, respectively. Similarly, the oxidized low-density lipoprotein receptor 1 (OLR1) gene, which plays an important role in the humoral immunity of the skin, and the plexin D1 (PLXND1) gene, which is highly expressed in tumor vasculature, were both found to be common targets of miR-27b, miR-1914, and miR-612.

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miR-612 suppresses stem cell-like property of hepatocellular carcinoma cells by modulating Sp1/Nanog signaling

Cited by 44 publications

References 47 publications

RETRACTED: MicroRNA‐612 inhibits cervical cancer progression by targeting NOB1

RETRACTED: MicroRNA‐612 inhibits cervical cancer progression by targeting NOB1

microRNA: The Impact on Cancer Stemness and Therapeutic Resistance

Gene Expression Profiling of MicroRNAs in HPV-Induced Warts and Normal Skin

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